Q: What is the difference between a mixed culture and a contaminated culture if they both contain more…
A: In microbiology , a bacterial culture is a growth of microorganisms which have been grown in a…
Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. What is…
A: The dilution factor is a term used to describe the ratio of the final volume over the aliquot…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: Find the amount of dehydrated medium needed to prepare 15 BUTTS, 20 SLANTS, and 25 BUTT-SLANTS.
A: Slants, Butt-slants, and butts are different types of media prepared for bacterial growth. Slants…
Q: Why does motility media contain a lower amount of agar than ordinary agar?
A: Agar is a phyco-colloid polymer of galactose monomers obtained from red algae (Gelidium and…
Q: Why is it important to keep the culture at the correct incubation temperature when measuring the…
A: Generation time is the time which is taken by the bacteria to double in number during a specified…
Q: Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.
A: Escherichia coli is the gram negative bacteria.
Q: Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when…
A: The oxidase is a test used in microbiology to identify bacteria that produce cytochrome c oxidase.…
Q: methods of sterilization
A: Sterilizationis the statistically complete destruction of all microorganisms including the most…
Q: CITRATE UTILIZATION TEST WAS PERFORMED IN A CULTURE MEDIA WHICH CONTAIN A STRONG BUFFER. AND EVEN A…
A: In this test citrate agar is used, where citrate is carbon source and NH4and ability of organism to…
Q: To prevent any signs of contaminants in the broth it is sterilized by heating. Sterilization by…
A: A broth refers to the growing media for microorganisms. It consists of ingredients that are known to…
Q: The optimum growth pH for a microorganism is usually: 4,5,7 or 8
A: pH is the scale on which the acidity or alkalinity is measured. It is the scale between 1 to 14.…
Q: Agar is a solidifying agent used in media preparation. What is its origin?
A: Agar gel is a polysaccharide which can become liquefied at temperatures ranges from 85°C to 90°C and…
Q: What is the physical appearance (solid, liquid, semi-solid) of following media: agar plate
A: The Agar plate is made from mixing agarose and a growth medium onto a petri dish. Agarose is in a…
Q: What are the advantages and disadvantages of the filter method in the analysis of water samples?
A: Utilising the membrane filter method, test is gone through the layer utilising a filter funnel and…
Q: Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes…
A: * Disinfectant is a chemical substance that inactivates or to destroy the microorganisms that are…
Q: why it is useful to plot the microbial growth from a growing microbial culture?
A: The term microbial growth refers to the growth of a population of microbe, it is an increase in the…
Q: Can culture media be placed in a freezer? Why or why not
A: Culture media are also called as growth media. They are mixture of nutrients and other substances…
Q: Which of the following water quality assay methods produce the greatest amount of biohazardous…
A: Introduction A biological hazard, often known as a biohazard, is a biological substance that…
Q: What concentration of agar is used in a semisolid medium for motility determination? How does that…
A: Agar is a polysaccharide that is obtained from marine seaweeds. Agar is mostly used in food and…
Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…
A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…
Q: Which of the following can most likely increase the yield of recrystallized benzoic acid? CHOICES:…
A: Recrystallization is a method that is used for the purification of solid compounds by the removal of…
Q: If a yellow halo is present around a colony on a mannitol salt agar (MSA) plate, the bacterium…
A: Mannitol salt agar (MSA) is used as a culture medium or growth medium. Growth medium is a medium…
Q: Solid and liquid media are commonly prepared based on manufacturer’s instructions. If no instruction…
A: Sterilization is a physical process that, in microbiology science, plays a vital role in preventing…
Q: Why does turbidity in a cultured media losses its reliability at high cell concentrations?
A: turbidity is used as a measure of cell density within a culture sample.
Q: Provide three reasons why the use of aseptic technique is essential when handling microbial cultures…
A: Aseptic techniques means using practices and procedures to prevent contamination from pathogens. It…
Q: why do We need to Wait for the iron Agar to set and then Add another thin layer of 45°C Iron Agar?
A: The test that uses iron agar is the Triple Sugar Iron (TSI) Test and is used to determine the…
Q: give one reason why is it necessary to keep stirring the agar medium mixture in a beaker when…
A: Agar is a heteropolysaccharide, which is composed of different carbohydrate units. Agar is obtained…
Q: What is one advantage of a pour plate over a streak plate?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: chemically defined media b. complex culture media
A: Media is a growth medium of solid,liquid and semi solid type. Diffarent types of media are used for…
Q: Heat-labile material to be introduced to culture medium was filtered using membrane filters with…
A: given that the heat labile material which is introduced to culture media is filtered using a…
Q: Of what practical importance are air borne microorganisms to the laboratory workers? What…
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Q: WHAT IS THE TYPE OF Growth patterns on Agar Slant and Broth Medium
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A: Bacteria culturing is done in order to grow the bacterial strains that are able to show resistance…
Q: Explain why the following steps are necessary during subculturing: Cooling the inoculating loop…
A: Subculture is the process of the removal of the media and transferring the cells from previous…
Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…
A: In microbiology, culture media is considered the growth medium that possesses the specific mixtures…
Q: If a bacteria is inoculated onto milk agar and an area of clearing is observed around the bacterial…
A: Milk agar or skim milk agar is used to differentiate and cultivate microbes based on their…
Q: Why should your solvent be evaporated from the sample placed on the sterile disk before you place it…
A: The antimicrobial activity of natural compounds like essential oils derived from plants can tested…
Q: You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. You then…
A: A serial dilution is any dilution in which the concentration is decreased by the same factor in each…
Q: What is diluent? Why is it useful for working with bacterial samples?
A: Dilution is done to decrease the concentration of the original sample that is being tested. Dilution…
Q: You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show…
A: Introduction :- A test to determine whether you have a bacterial infection is a bacterium culture.…
Q: Which is more Suitable for long-term storage: agar slant or plate? Explain why?
A: In order to isolate the bacteria from samples, they need to be inoculated into a growth medium which…
Q: List 5 other methods of sterilization and describe the principle/s and when each method is used.
A: (Please note that, you provided more than one question, I can only answer one question at a time,…
Q: What is the meaning of the word sterile? What would happen if culture media freshly prepared were…
A: Sterile It refers to the state of being free from any bacteria or any other living microorganisms.…
Q: Agar is a solidifying agent used in media preparation. How and why does the agar concentration in…
A:
Q: What are the primary uses of the following preparations of culture media? Agar regular slant Agar…
A: Hello. Since your question has multiple sub-parts, we will solve the first three sub-parts for you.…
Q: What is the difference between a disinfectant and an antiseptic?
A: As per our company's guidelines we are supposed to answer single question at a time, please repost…
Q: An unknown bacterium produces colorless colonies when inoculated onto an EMB plate. Predict what you…
A: EMB stands for Eosin Methylene Blue. It is a selective stain used for the growth of gram-negative…
Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50°C?
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- If a yellow halo is present around a colony on a mannitol salt agar (MSA) plate, the bacterium cannot ferment mannitol. true or falseWhy does motility media contain a lower amount of agar than ordinary agar?To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. How did you calculate it?. Single line text.
- how do microorganisms such as bacteria eliminate harmful reactive oxygen species? Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.Agar is a solidifying agent used in media preparation. How and why does the agar concentration in semisolid media differ from conventional solid media?Aside from application of heat, name other methods of sterilization and give examples of materials sterilized in each method
- What are the advantages and disadvantages of the filter method in the analysis of water samples? Why is the incubation temperature critical for this test?During media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?A culture medium is comprised of the following components: 0.5% peptone, 0.3% meat extract, 1.5% agar, and distilled water. If you are asked to prepare 765 mL of culture media, how much meat extract would you need?