Gram Stain Results: Staphylococcus aureus (a Gram-positive coccus) Escherichia coli (a Gram-negative rod) Acid Fast Stain Results:

Q: The first step in identifying an organism from a pure working culture is to perform a(n) __________…

A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: Explain why only gram-negative cells undergo decolorization during the gram staining procedure.

A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…

Q: Crystal violet is an example of what type of stain?

A: A stain is used to clearly view a specimen under the microscope. For staining, a smear of the…

Q: The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with…

A: Mycobacteria are immobile, slow-growing (doubling time = 24 h), rod-shaped, gram-positive bacteria…

Q: Please discuss why staining is important in visualizing microbes under the microscope. What…

A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…

Q: Describe the bacteria observed in different staining procedures. Please refer to the photo -…

A: Bacteria can be stained by a variety of stains so that they can be visualised in contrast to the the…

Q: Which of the following are true about a differential stain. There may be more than one answer!…

A: A microscopic organism made of one cell is referred to as a prokaryote. Prokaryotic organisms do…

Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…

A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…

Q: shape

A: There are different shapes of bacteria and different classes of bacteria on its shape These shapes…

Q: Match the organism with the method of staining best used to identify it. Salmonella Typhi…

A: Stain It is a substance that adheres to a cell, giving the cell color. The presence of color gives…

Q: Name all the reagents used in Gram-staining and their roles in the staining procedure.

A: Staining is a strategy used to improve contrast in examples, for the most part at the minuscule…

Q: Streak for Isolation Successful or unsuccessful? (How could you tell?) Pigmented or non-pigmented?…

A: Microbial culture techniques are applied to determine what type of microbial species a sample…

Q: In gram staining, if bacteria retain the primary stain after decolorizing agent treatment. Then the…

A: Answer: GRAM STAINING = It is the differential staining method to differentiate between gram…

Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…

A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…

Q: If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would…

A: The acid-fast staining technique is a differential staining technique. The bacteria which get…

Q: What color will a gram-negative cell be after Gram staining bythe conventional method?

A: Danish bacteriologist Hans Christian Gram developed the Gram staining method. This staining…

Q: Explain what happens to Gram-positive and Gram-negative bacterial cells at each step of the…

A: The process of Gram Staining on any sample requires four basic steps that include applying a primary…

Q: If the decolorizing step was omitted in the Gram-staining procedure, what color would you expect…

A: Gram staining is performed on the basis of differential characteristics of the cell wall in…

Q: List the  steps in a Gram stain.

A: Introduction: The principle of Gram's stain depends upon the Gram's reaction. The Gram's reaction…

Q: Which step of the gram stain procedure is most critical?why?

A: Gram staining is one of the most common laboratories test to identify the different bacterial…

Q: Based from the results obtained from Gram staining, explain why bacterial cells retained or changed…

A: Staining is a technique that is used to enhance the contrast of the microscopic image through the…

Q: Match the expected result (purple, red, or colorless) to the following descriptions of Gram-stained…

A: Various characteristics such as shape,arrangement of cells, formation of capsule or spores can be…

Q: Step-by-step smear preparation of Grams stain

A: Gram staining is a laboratory technique that is used to culture bacteria and identifies two kinds of…

Q: Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and…

A: Bacteria are small, microscopic and unicellular organisms that can live in variety of environment.…

Q: If the decolorizing step was omitted in the Gram-staining procedure, what color would you expect…

A: Gram staining helps in differentiation between gram positive and gram negative bacteria. Procedure…

Q: Submit screenshots of the Gram stain (1000x total magnification) and Endospore stain (1000x total…

A: Gram staining is used to differentiate between the gram positive and negative bacteria which appear…

Q: List the reagents of the Gram stain technique in order and their general role in the staining…

A: Gram Stain is used to differentiate the gram positive and gram negative bacteria. The gram positive…

Q: Define the term gram staining.

A: Bacteria are a diverse group of microorganisms with a wide range of shapes, sizes, structures, and…

Q: The acid fast technique is also known as:  A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C.…

A: Acid fast bacteria are gram positive bacteria . They are unique among prokaryotes and normal gram…

Q: list the stains and decolorizing agent used in acid fast staining method.

A: Certain bacteria have a physical property known as acid fastness. Property:- Resistance to…

Q: Regarding the Gram stain procedure: whether Gram-positive or Gram-negative, cells may appear…

A: Q. Regarding the Gram Strain procedure: Whether Gram positive or Gram negative,cells may appear…

Q: move the images of bacterial cells into the chart to illustrate the color of gram-positive and gram…

A: answer given below

Q: List the step-by-step procedure of Gram staining.

A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…

Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…

A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…

Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…

A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…

Q: Gram straining procedure. Indicate the following information A. Shape........ B. Stained color…

A: On the basis of gram staining, we can differentiate between two types of bacteria, one with thick…

Q: Evaluate the following preparations in terms of providing informationon microbial size, shape,…

A: Staining is an important step in observing the microbes under a microscope. they allow to the…

Q: Decolorization in Gram-staining removes safranin from gram-negative cells   True False

A: Bacteria are tiny, single-celled life forms that exist in their millions, in each climate, both…

Q: Which of the following is/are true regarding the acid-fast stain? (There may be more than one…

A: Answer  First option  Non-acid-fast microbes appear blue in a completed acid-fast stain.

Q: Gram stain: Cell shape: Based on your above results, indicate which tests need to be done & what…

A: Microbiology is the branch of science which deals with the study of organisms which are too small to…

Q: What are the expected result after gram staining?   a. Crystal Violet = b. Gram Iodine =  c.…

A: Gram staining is a type of staining technique used to differentiate bacteria based on their cell…

Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?

A: Introduction  We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…

Q: The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in…

A: Acid fast bacteria are those which responds to acid fast staining . Most of the members of…

Q: A staining procedure in which one reagent is used and the bacteria takes up the stain while the…

A: Staining is a technique used to enhance contrast in the sample generally at microscopic level. There…

Q: In preparing bacterial smear, why do we need to pass the slide over the flame?

A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…

Q: What staining technique is used here? What is the primary stain, decolorizing agent, and counter…

A: Gram Staining is the common, important, and most used differential staining techniques in…

Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…

A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…

Q: List the steps of the gram staining procedure in proper sequence and indicate the color of…

A: Gram staining is a method of staining used to distinguish and classify bacterial species into two…

Q: For each of the following stains, list each of the chemicals used (in order), briefly describe what…

A: In Microbiology staining is needed in order to properly visualize the internal membranes of the…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.