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Which of the following are positive for the production of indoor from the amino acid tryptophan?
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- Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer. In addition to H2S production and motility, for what other test can SIM medium be used?A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture into a 9 ml LB medium. After this, from 10-4 and 10-5 dilution tubes 100µl were plated onto LB agar plates. Upon overnight incubation at 37°C, 200 colonies were counted in 10-4 and 22 colonies were present on 10-5 plates. How many colony-forming units were present per ml of the original culture? If the formula for CFU/ml =no. Of colonies/dilution factor*volume of culture plateAssume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If we can order 5 milligrams of fibronectin for $871.00, how much would it cost to have enough to use every well of the 24-well culture plate?
- What is the result of this biochemical test in the gelatin deep?cWhich enzyme is this testing for? What tool is used to inoculate this medium?What are the advantages of the API 20E test compared to regular biochemical tubemediaHello, I want you pleaseeee to make a dichotomous key based on provided experiments to identify a an unknown bacterium mixture. It can be E. coli, B. Bulgaris, B subtilis, b aerogenes, m leuteus, s. Aureus, and s epidermisis. Make it based on these possible experiments: phenol red broth, mr/vp test, catalase test, oxidase test, nitrate reduction test, citrate utilization test, Malonate utilization test, decarboxylation test, starch hydrolysis test, dna hydrolysis test, lipid hydrolysis test, gelatin hydrolysis test, urea hydrolysis, SIM medium, triple sugar iron agar test, blood agar test. So far we have run a MAC agar and PEAA plate of the bacterium
- a.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?Kligler’s iron agar and SIM are multiple test media.. What component of both media allows you to detect the preceding characteristic?You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture. What is the first step to prepare your Kirby-Bauer test?
- Following electrophoresis of the two pGlo mini-preparation samples (both digested and undigested), the following agarose gel image was obtained. provide an accurate and detailed figure, describing the image and what it depicts.Background: The following results were obtained from a broth dilution test for microbial susceptibility. What is the minimum bactericidal concentration of this antibiotic and explain what you based your answer on?In this experiment of polyphenoloxidase, why does the longer the time, the darker the color is ithe result of the unheated sample?