Why should excess bromine water be avoided in the test for free Tryptophan?
Q: Why does the oxidase test have to be read quickly?
A: The Oxidase test is a biochemical test for the identification of bacteria. The test is mainly…
Q: Why is the mucic acid test specific for the presence of galactose?
A: Galactose is a monosaccharide with the structure shown below: It is a component of disaccharide,…
Q: What would be the result of hexan-2-one or hexan-3-one on Brady's and Iodoform test?
A: Principle of Brady's Test: 2,4-dinitrophenyl hydrazine(DNP) of Brady’s reagent reacts with carbonyl…
Q: What is the result of tube A in this Voges-proskauer test? What are the reagents of this test? What…
A: Voges-proskauer test is performed to differentiate between facultative anaerobic enteric bacteria.
Q: Give the principle involved in the ninhydrin test. What is its purpose?
A: The principle involved in the ninhydrin test is that the two molecules of ninhydrin react with the…
Q: What is a folin-ciocalteau reagent and how can it be used to determine the concentration of uric…
A: Uric acid is the end product formed by the catabolism of purine bases. The normal range of uric acid…
Q: How would you interpret this catalase test
A: Catalase is an enzyme which can convert hydrogen peroxide to oxygen and water and thereby decrease…
Q: Does Escherichia coli provide positive or negative results for the lysine decarboxylase and…
A: Ornithine decarboxylase test detects whether bacteria uses ornithine amino acid for carbon source…
Q: What is the mechanism of the reaction of the Bradford reagent with proteins? (explain in not less…
A: The Bradford protein assay is used to measure the concentration of total protein in a sample.
Q: In urea hydrolysis what emd product is specifically being tested for in this test? Is the final ph…
A: The general formula or urea is NH2CONH2. Urea breakdown requires the enzyme urease. It is primarily…
Q: Between Proteus mirabilis and Proteus vulgaris which are positive or negative for ornithine…
A: ornithine decarboxylase test is a special kind of biochemical test that helps to determine whether…
Q: What other test(s) are used in parallel to Benedict’s test?
A: Reducing sugar: a. Carbohydrates that contain free aldehyde or ketone group and are in hemiacetal or…
Q: Why did Anfinsen use urea and β-mercaptoethanol in hisexperiments?
A: Christian Anfinsen conducted an experiment in the 1950s and determined that specific amino acid…
Q: What effect does the H2O2 have on bacteia in the catalase test?
A: The production of toxic substances like hydrogen peroxide due to metabolic activities can be lethal…
Q: Differentiate Widal from Weil-Felix test.
A: The Weil-Felix test detects typhus and specific rickettsial infections. Rickettsia is bacteria…
Q: Which of the following are positive for the production of indoor from the amino acid tryptophan?
A: indole is produced by reductive deamination from tryptophan through the intermediate molecule indole…
Q: Describe two methods to test for the enzyme urease.
A: Urease is an enzyme that catalyzes the hydrolysis of urea forming ammonia and carbon dioxide. They…
Q: What is Zeta Sedimentation Ratio?
A: As per our answering policy, we can only answer one question at a time. Please post the other…
Q: What is the principle involved in the Acrolein test? What is its purpose?
A: Qualitative tests are the chemical or physical tests involved in identification of certain…
Q: Egg white solution when mixed with boiled pancreatic extract will be (+) or (-) in both Biuret and…
A: Biurete test: A Biuret test is a chemical test used to determine the presence of a peptide bond and…
Q: How is the precipitation of proteins by alcohol applied in the fixing of tissues for histological…
A: Histology is generally the study of tissue types for identification and diagnosis purposes for cell…
Q: How do you determine protein concentration using bicinchoninic acid assay?
A: Proteins are polymers that are composed of amino acids. Amino acids are in turn made up of a central…
Q: Why is the mucic acid test specific for the presence of galactose and Is glucose always present in…
A: Galactaric acid is another name for mucic acid. Galactose is a sugar which tastes less sweet…
Q: A solution of flycine and a solution of albumin were prepared with the same concentration (g/L). One…
A: The ninhydrin test is a chemical that is used to determine the presence of alpha amino groups in the…
Q: Why does the oxidase test must be performed from blood agar or another medium without a fermentable…
A: Biochemical tests are used to identify microbial diversity based on variations in the biochemical…
Q: Why sucrose gives negative Benedict test?
A: Benedict's reagent is a chemical reagent which is a mixture of sodium carbonate, sodium citrate, and…
Q: How smith's test gives a green ring result in urine as a presence of bile salts? What is the…
A: Smith's test is to check the bile pigment.
Q: What is the purpose of PSP (Phenolsulfonpthalein) Test?
A: Please follow step 2 for detailed explanations.
Q: Complete the table for Watson-Schwartz Test Results for porphobilinogen and urobilinogen
A: Watson-Schwartz test is a qualitative screening test for diagnosing acute intermittent porphyria…
Q: What are the colour changes occur to the reducing sugars after benedict test is performed? Please…
A: Reducing sugar is a sugar that is capable to act as a reducing agent. Reducing sugars are the ones…
Q: Can galactric acid test/mucic acid test be used to determine the presence of milk in milk-chocolate…
A: A nutrient-rich white fluid secreted by the mammary glands of the mammals is referred to as milk.…
Q: A series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a…
A: The Bradford assay is based on the fact that the absorbance maximum for an acidic solution of…
Q: Why is hudroxychloroquine or chloroquine detrimental against COVID-19?
A: The coronavirus outbreak, which emerged in December 2019 in Wuhan, China, has developed into a…
Q: A 35-year-old pregnant woman had a regular check-up and the doctor found the sugar slightly elevated…
A: Diabetes is the most common metabolic disorder that causes a high level of blood sugar. Glucose is…
Q: What is the result of this biochemical test in the gelatin deep? Which enzyme is this testing for?…
A: Gelatin Hydrolysis Test: Gelatin is a collagen-derived protein obtained from the connective tissues…
Q: what are the Amino Acids that test positive for Ninhydrin test? what is the principle of detection?
A: All the alpha amino acids test positive for ninhydrin test.
Q: Using SP-Sepharose as ion exhange resin, indicate the starting and ending pH for the narrowest…
A: Ion exchange chromatography is applicable for the separation of charged molecules. In this…
Q: Before using the API® 20E System, what test must be performed to confirm the identity of your…
A: Bacteria are of two classes gram-negative and gram-positive bacteria. They are classified on the…
Q: What are the different test for non-reducing sugar?
A: Carbohydrates or carbs are maconutrient consisting of Carbon, hydrogen and oxygen atoms. In nature…
Q: purpose of the Methyl Red and Voges-Proskauer Test. What makes each one unique and why?
A: The methyl red reagent is a solution that detects the acidic pH of the culture. Voges-proskauer test…
Q: Describe Ninhydrin test for proteins with the reaction
A: Qualitative tests help to detect the presence of substances by giving visible reactions such as…
Q: Is there a possibility for lactose to give a positive Mucic Acid test? Explain.
A: Monosaccharides upon treating with oxidizing agents here, nitric acid yield dicarboxylic acids by…
Q: What amino acid present in proteins is responsible for the reaction in Hopkins- Cole test ?
A: Amino acids are the monomer of the protein. Each amino acid has a carboxyl group, an amino group, a…
Q: IN A BIURET ASSAY TWO PROTEIN SOLUTION CONTAINING 150MG/ML AND 180MG/ML SHOWS THE SIMILAR…
A: Biuret test of protein is to done to quantify the proteins or to detect the concentration of…
Q: How to explain that milk protein casein curdles (precipitates) during boiling if the milk is sour?
A: Casein is precipitated by simply adjusting the pH of the milk to be sufficiently acidic that the…
Q: Both Glucose and galactose are known to be epimers. To differentiate these two sugars, what test…
A: There are many biomolecules are found in the human body. Carbohydrates, proteins, lipids, and…
Why should excess bromine water be avoided in the test for free Tryptophan?
Step by step
Solved in 2 steps
- Is there a possibility for lactose to give a positive Mucic Acid test? Explain.What are the colour changes occur to the reducing sugars after benedict test is performed? Please mention the colour change observed for each reducing sugars in benedict testWhich is the commonly occurring sugar in milk? Will the milk containing this sugar gives a positive result for the test " Detection of sugar in Milk"?
- What is the interpretation and reaction catalyzed of this GPT enzyme test?.Both Glucose and galactose are known to be epimers. To differentiate these two sugars, what test should be used? Explain the answer.Would the ninhydrin test useful and reliable for showing the presence or absence of proteins?