Which of the following ingredients does not belong in a sequencing reaction? (A ddNTPs B Primer C Ligase D DNA polymerase E DNTPS F Endonuclease

Q: A circular DNA of 40 kb (40,000 bases) length is cut with a restriction enzyme whose precise…

A: The probability of a restriction enzyme to be found in a DNA is 4n, where n is the number of bases…

Q: Polymerase chain reaction (PCR) carries out DNA replication in a test tube. Which of the following…

A: Introduction Deoxyribonucleic acid, or DNA, is an organic molecule with a complex molecular…

Q: Select all of the following that need to be in a polymerase chain reaction that will successfully…

A: Polymerase chain reaction (PCR) is a molecular technique used by scientists to multiply particular…

Q: Which of the following steps is NOT used in whole genome sequencing? Select one alternative: O…

A: WGS stands for whole-genome sequencing and is a method for examining complete genomes. Identifying…

Q: To amplify a section of DNA using the polymerase chain reaction (PCR), all you need to load into the…

A: PCR is the technique in which DNA molecules are replicated outside the cell, that is, in in-vitro…

Q: Identify the CORRECT sequence of steps that occurs during every cycle of PCR. 1. The primers…

A: PCR, short for Polymerase Chain Reaction is a molecular technique in which multiple copies of a gene…

Q: Which of the following is NOT a characteristic of PCR primers?  Short synthetic oligonucleotide…

A: Polymerase chain reaction is a procedure in which a strand of DNA is amplified by using short RNA…

Q: If you amplify a single double-stranded (ds) DNA template molecule using 25 PCR cycles, how many ds…

A: The polymerase chain reaction is a method for cloning a particular piece of DNA to make virtually…

Q: Which of the following is not a requirement for a PCR mix? O a. Taq polymerase O b. Excess…

A: Polymerase chain reaction Polymerase chain reaction or PCR , as it is commonly known who invented by…

Q: Which of the following enzymes has 5’ to 3’ exonuclease activity? DNA ligase DNA polymerase I…

A: To shift DNA strands, the 5′ to 3′ exonuclease activity can be used with the polymerization…

Q: Below is a segment of DNA which you wish to amplify using PCR (polymerase chain reaction). The…

A: The DNA polymerase always bonds with the free 3'-OH of the primer and elongated the new DNA strand…

Q: The replication errors can be caused due to O a. DNA polymerases increase proofreading. O b.…

A: Since there are multiple questions in this particular question, I'll answer the first one for you.…

Q: A cloned fragment of DNA was sequenced by using the dideoxy method. A part of the autoradiogram of…

A: Sanger sequencing, often called chain-termination sequencing, is a DNA sequencing technology…

Q: Which of the following is NOT needed to perform a PCR reaction? A) DNA primers B) deoxyguanosine…

A: Components required for PCR include a DNA sample which acts as a template , DNA primers, free…

Q: DNA polymerases make errors in matching as DNA is synthesized. These errors can be detected and…

A: DNA replication is the process by which two exact copies of DNA are made from an original DNA…

Q: the RNA primer at the beginning of each Okazaki fragment is removed by : A-DNA polymerase I  B-DNA…

A: Replication Replication is defined as the process of synthesis of new DNA from the existing DNA…

Q: Each cycle of amplification in PCR involves all of the steps EXCEPT: A annealing of…

A: In the 1980s, Kary Mullis developed the PCR (Polymerase Chain Reaction) method. PCR makes use of DNA…

Q: Which of the following enzymes are NOT used in recombinant DNA technology O A. Eco RI O B. Ligase O…

A: INTRODUCTION Recombinant DNA (rDNA) is a process of joining DNA molecules from two different sources…

Q: In a PCR experiment, the temperature for primer annealing depends on the length of the primers. the…

A: a and b, but not c Melting temperature or temperature monitoring is the temperature at which double…

Q: PCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to…

A: Introduction  The polymerase chain reaction (PCR) is an in vitro technique for amplifying a…

Q: you performed a sanger sequencing on a particular segment of DNA. Explain at the molecular level…

A: Effect on gel profile when we either forgot to add ddNTPs Or add more ddNTPs in test tubes during…

Q: Human DNA and a particular plasmid both have sites that are cut by the restriction enzymes HindIII…

A: Answer- Restriction enzymes or molecular scissors cut the DNA into segments. It is highly specific…

Q: The purpose of using a washing soap in DNA extraction experiment is to O a. Lyse the cells to…

A: DNA extractions is a method to isolate DNA from the nucleus of given cell culture. The four steps of…

Q: The RNA primer at the beginning of each Okazaki fragment is removed by

A: Introduction: The small pieces of the discontinuously synthesized DNA are basically known as Okazaki…

Q: You have two PCR primers: Forward- 5' TGAGCTAGGC 3' and Reverse- 5' GGTTCAGTCAG 3'. Show the binding…

A:

Q: Which enzyme is used in Sanger sequencing reactions? O A. DNA polymerase O B. S1 endonuclease O C.…

A: Introduction :- Sanger DNA sequencing is commonly employed in research to target smaller genomic…

Q: Following PCR amplification of a disease gene, you perform sequence analysis using a primer 20…

A: After performing sequencing through gel electrophoresis DNA fragments are organized according to…

Q: Which of the following DNA-synthesizing enzymes has 5' to 3' polymerization activity? Mark all the…

A: Polymerization is defined as the process in which tiny molecules known as monomers will chemically…

Q: GAATTC GAATTO CITAAG CITAAG double-stranded DNA DAATT DAATTO CTAA G CTAA GI AAT TO CTTAA GAATTO…

A: The cloning is routinely used in biotechnology laboratories and it is the process by which a foreign…

Q: PCR requires all of the following EXCEPT-- Select one: a. DNA of interest b. DNA polymerase c.…

A: PCR is a molecular method for amplifying DNA sequence in laboratory diagnosis. It is a versatile…

Q: PCR primers Below is a 300 base pair fragment of DNA. The top strand is written in the 5' to 3'…

A: Introduction A primer is a single-stranded nucleic acid that all living organisms utilise to start…

Q: Which of the following ingredients precisely controls which small section of a very large DNA…

A: The acronym PCR stands for polymerase chain reaction. It's a test that looks for genetic material…

Q: Polymerase chain reaction (PCR) is a technique used to amplify a fragment of DNA. When conducting…

A: PCR or Polymerase Chain Reaction is the procedure of amplifying a DNA segment. It was invented by…

Q: Which of the following is required in running a PCR reaction? Promoter Primase DNTPS DNA polymerase…

A: PCR is polymerase chain reaction , which is a biotechnology tool used in DNA amplification.

Q: g best describes the complete sequence of steps occurring during every cycle of PCR? I-The primers…

A: A laboratory method used to make many copies of a specific piece of DNA from a sample that contains…

Q: What is a DNA Ladder? a solution added to a DNA sample to give it color for an electron microscope.…

A: DNA:  The hereditary substance in humans and almost all other animals is DNA, or deoxyribonucleic…

Q: Arrange the following steps in the sequence they would happen in a DNA cloning experiment. a.…

A: DNA is an important biomolecule present in the cell as it is responsible for various traits…

Q: An individual’s unique set of_______ can be used in DNA profiling. a. DNA sequences c. SNPs b. short…

A: Introduction  In the genome, there is usually two types of nucleic acid sequence present. One is…

Q: Which of the following is/are not required for DNAreplication to occur?a. DNA polymerase d.…

A: DNA (short for deoxyribonucleic acid) is a double helical structure (with two DNA strands). A…

Q: Which of the following enzyme is used to join bits of DNA? O ligase primase O DNA polymerase O…

A: DNA replication occurs in the nucleus of the cell. DNA replication leads to the production of…

Q: Which of the following enzymes has the highest functional error?  a) DNA Polymerase I b) DNA…

A: The proofreading activity of enzymes helps in reducing the number of errors. Proofreading activity…

Q: Why is the temperature inside the thermal cycler lowered to 55°C during the PCR process? Select one:…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Which of the following is not essential to carry out the polymerasechain reaction?a. primers b. DNA…

A: PCR is the polymerase chain reaction and is used to make various copies of a specific region of DNA…

Q: Which of the following is the correct order in extracting the DNA?   I. Washing of DNA II. Tissue…

A: DNA act as genetic material in most of organism . It is two stranded , ladder like structure . DNA…

Q: Which of the following tools of DNA technology is incorrectlypaired with its use?(A)…

A: As in the recent times, the approach towards genetics has gone up a high level. Various techniques…

Q: An enzyme to add nucleotides specifically at the ends of chromosomes would be: Question 6 options:…

A: Chromosomes are the carrier of hereditary DNA molecules and have a thread-like appearance. They are…

Q: Which of the following is NOT a function of DNA polymerase I in E. coli? Question 1 options: A) 5'…

A: Answer: C) Helicase

Q: PCR is an exponential copying of the template strands and can be represented by the function: y = a…

A: PCR is a biochemical process capable of amplifying a single DNA molecule into millions of copies in…

Q: Chimeric DNA true are all except - a) Formed by linking DNA fragments of unrelated nis ood l nsvi…

A: Chimeric  DNA is also known as recombinant DNA which is formed by linking DNA fragments of two…