Identify the CORRECT sequence of steps that occurs during every cycle of PCR.I. The primers hybridize to the target DNA.II. Binding of DNA polymerase to the target DNA.UI. Extension of primers to make a copy of the target DNA.IV. The mixture is heated to a high temperature to denature the double stranded target DNA.A. I, II, III, IV.B. II, I, IV, II.C. IV, I, II, II.D. IV, II, I, III.

PCR, short for Polymerase Chain Reaction is a molecular technique in which multiple copies of a gene…

Identify the CORRECT sequence of steps that occurs during every cycle of PCR. 1. The primers hybridize to the target DNA. II. Binding of DNA polymerase to the target DNA. III. Extension of primers to make a copy of the target DNA. IV. The mixture is heated to a high temperature to denature the double stranded target DNA A. I, II, III, IV. B. II, I, IV, III. C. IV, I, II, III. D. IV, II, I, III.

Identify the CORRECT sequence of steps that occurs during every cycle of PCR. 1. The primers hybridize to the target DNA. II. Binding of DNA polymerase to the target DNA. III. Extension of primers to make a copy of the target DNA. IV. The mixture is heated to a high temperature to denature the double stranded target DNA A. I, II, III, IV. B. II, I, IV, III. C. IV, I, II, III. D. IV, II, I, III.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 12QP

See similar textbooks

Similar questions

Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.
Regarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genes
Would it be possible to use human polymerase for the PCR reaction? a. No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur. b. No, because human polymerase cannot be extracted from cells to use in a lab setting. c. Yes, because we are using human DNA as the template DNA. d. Yes, because human polymerase can add bases to a template strand without a primer.
Explain briefly within 5 sentences each 1. a. What is PCR? Explain/relate its importance in DNA marker analysis. 1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.
Choose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)? a. PCR requires a copy of RNA that serves as a template b. Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA c. Annealing requires cooler temperatures than denaturation d. Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA e. PCR is a quick way of using minute quantities of DNA to create millions of copies
A) What are the three main steps involved in PCR? Include temperatures and descriptions of each step. B) Explain why RFLP can produce many bands on an electrophoresis gel and PCR (one set of primers) will only produce one or two bands on a gel for the same genome.
Which of the following best describes the complete sequence of steps occurring during every cycle of PCR? I-The primers hybridize to the target DNA. II-The mixture is heated to a high temperature to denature the double stranded target DNA. III-Fresh DNA polymerase is added. IV-DNA polymerase extends the primers to make a copy of the target DNA. A) II, I, IV. B) I, III, II, IV. C) III, IV, I, II. D) III, IV, II. E) II, III, IV.
Explain how oligonucleotide primers in the Polymerase Chain Reaction work (PCR)
During DNA isolation, you incubated the tissue sample at 55°C for 60 minutes, followed by incubation at 95°C for 10 minutes. What happens to the DNA molecules in this last 95°C incubation? What would happen to the PCR if this step was omitted?
Choose the one answer that fits best. Which statement regarding Molecular Biology is NOT correct (videos)? a. Taq Polymerase was isolated from an organism found in Yellowstone Park b. Restriction enzymes leave sticky ends c. DNA sequencing allows us to read DNA sequences d. Restriction enzymes cut DNA at specific sites e. EcoRI and HindII are commonly used polymerases
All are true about PCR except A. Automated PCR machine called a thermal cycler B. A thermostable DNA polymerase is required C. Primer extension occurs at 72˚C D. Annealing involves binding of primer between 90-98˚C
Compare and contrast polymerase chain reaction (PCR) to DNA replication in a cell.