Which of the following is INCORRECT about protein denaturation? O Denaturation destroys the enzymatic activity of an enzyme. O Protein denaturation is a necessary first step in SDS-PAGE. O Mercaptoethanol destroys the disulfide bridges in a protein. O Boiling proteins in buffer cleaves peptide linkages.
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- Which of the following is an example of protein denaturation? * A. Amino acids fold into repeating patterns due to hydrogen bonding of the peptide backbone. B. Several amino acids are joined together together via peptide bonds. C. A protein binds with with a substrate, lowering the activation energy of reaction. D. A protein is exposed to extremely high heat, causing it to lose its secondary structure and be left with only its primary structure. E. Results to unfolding, partial or incomplete disorganization of the protein's secondary and tertiary structure.Which of the following statements are correct about protein folding (select all that appy)? A. Protein folding is a very slow process with most proteins requiring >30 min to go from unfolded to native conformation B. The mature two-chain form of insulin spontaneously folds into the native conformation C. A sharp transition in the presence of denaturants (See Figue 8.1) suggests the protein folds without intermediates D. Proteins randomly sample each possible conformation before arriving final native state E. Proteins with disulfide bridges tend not follow a simple two-state folding mechanism.Which of the following is true regarding proteins A. Absorbance of proteins is directly proportional to the molecular weights B.Proteins react with biuret reagent because of their side chains C. two out of the twenty amino acids can absorb at 280nm D. In proteins the average weight per amino acid residue always varies
- Which of the following statements is TRUE when aspartic acid in a protein is positioned in proximity with glutamic acid? The pKa of aspartic is increased. The proton dissociation from aspartic acid side chain is favored. The pKa of aspartic acid is decreased The pKa of aspartic acid is unaffected.Which of the following statements about chaperones is FALSE? a. Chaperones prevent incorrect interaction (i.e. aggregation) within and between non-native polypeptides thereby increasing the yield b. Chaperones are proteins c. Chaperones can greatly increase the rate of protein folding, and therefore, chaperones are enzymes. d. Chaperones do not specify correct folding e. Chaperones prevent aggregation of unfolded proteinsWhich of the following enzymes creates a covalent bond that links amino acids in a polypeptide? Protein disulfide isomerase Hsp70 Peptidyl-prolyl isomerase Calreticulin
- Which of the following statements concerning complete protein hydrolysis is incorrect? Primary structures are preserved Tertiary and secondary structures are disrupted All peptide bonds are broken Water is a reactant in the processWhich of the following statements best describe(s) the mechanism by which correct protein folding takes place once the misfolded protein binds the open ring of groEL? Select all that apply. The groEL ring closes to isolate the protein and provide enough time for the protein to properly fold on its own. b and d The groEL ring closes to provide a hydrophilic space to isolate the protein and inhibit its aggregation with others until properly folded. The groEL ring closes to isolate the protein and provide steric hindrance that mechanically refolds the protein. The groEL ring closes to isolate the protein and decode the information necessary to achieve the correct three-dimensional structure. None of the above I picked "The groEL ring closes to provide a hydrophilic space..." but it was incorrect..Which way is more common practice to characterize the strength of a binding reaction between a protein and its ligand? Group of answer choices By its binding free energy, delta G By its equilibrium association constant K(A) By its equilibrium dissociation constant K(D) By the rate at which the biding reaction proceeds
- The pKa of an acid depends partly on its environment. Predict the effect of each of the following environmental changes on the pKa of a glutamic acid side chain. (a) A lysine side chain is brought into proximity. (b) The terminal carboxyl group of the protein is brought into proximity. (c) The glutamic acid side chain is shifted from the outside of the protein to a nonpolar site insideReductase is the Fe protein composed of two: polypeptide chains linked by three disulfide bonds. polypeptide chains linked by a 4Fe–4S cluster. α subunits and two β subunits. polypeptide chains linked by a 2Fe–2S cluster.Of the following amino acids, which 3 are most likely to be found on surface of a single protein. a) Arg b) Trp c) Cys d) Phe e) Leu f) Lys