Why might adding a strong reducing agent like Dithiothreitol (DTT) help denature an extracellular protein? Disulfide bonds might be reduced that might be critical in stabilizing its structure O Disulfide bonds might be oxidized that might be critical in stabilizing its structure Disulfide bonds formed inside the cytoplasm can be oxidized O Cysteines can be reduced to form stabilizing disulfide bonds

Why might adding a strong reducing agent like Dithiothreitol (DTT) help denature an extracellular protein? Disulfide bonds might be reduced that might be critical in stabilizing its structure O Disulfide bonds might be oxidized that might be critical in stabilizing its structure Disulfide bonds formed inside the cytoplasm can be oxidized O Cysteines can be reduced to form stabilizing disulfide bonds

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter3: Cell Structure

Section: Chapter Questions

Problem 8SQ: In a lipid bilayer, the of all the lipid molecules are sandwiched between all of the __________. a....

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Which of the following levels of protein structure may be affected by hydrogen bonding? (a) primary and secondary (b) primary and tertiary (c) secondary, tertiary, and quaternary (d) primary, secondary, and tertiary (e) primary, secondary, tertiary, and quaternary
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At what level of protein structure does each of the following denaturation act? a. heat b. strong acid c. saturated salt solution d. organic solvents (e.g., alcohol or chloroform)
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A membrane-spanning protein segment has to adopt a regular secondary structure, because— Secondary structures facilitate the aggregation of nonpolar groups to increase stability. The hydrogen–bonding requirements of the polypeptide backbone have to be satisfied. α helix and β sheet have dimensions perfectly fit into the space created by the lipids. The regular structural features make it easy to align with lipid molecules in the membrane. Secondary structures can be packed tightly to minimize the surface areas.
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