Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0 ml B. spreading of the inoculum is done using a disinfected glass L-rod C. A pre-solidified medium is required prior to inoculation of the sample. D. A and B
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
1. Which of the following is NOT true of spread plating method?
A. the maximum inoculum allowed is 1.0 ml
B. spreading of the inoculum is done using a disinfected glass L-rod
C. A pre-solidified medium is required prior to inoculation of the sample.
D. A and B
2. Plates inoculated with bacteria are inverted during incubation to:
A. prevent contamination
B. limit the colony growth
C. A and B
D. A and C
E. All of the above
F. None of the above
Step by step
Solved in 3 steps
