Which of the following is true of nanometer a a nanometer is about the size of a common bacterial cell b cells come in a wide variety of sizes and shapes b the nanometer is the most common measurement used in measuring the whole cel, ca nanometer is equivalent to 10 A d a nanometer is one millionth of a meter e none of the above
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- Which of the following is false about counting viable cells? A. CFU(Colony forming unit) are counted instead of individuals cells B. Only 30-300 CFUs are statistically valid C. Colonies growing on the plate represent a fraction of the live cells in the original sample D. You can determine the number of live cells in a broth culture E. It is necessary to multiply the by CFUs to determine the number of viable cells in the original sample2. You have two microorganism samples and trying to determine characteristics they contain. You determine sample M requires an environment found in a hot spring. While sample P requires rat liver cells to survive. Based upon this information please classify what type of sample M and P are. Provide the term to each and define in detail. (hint for sample P: Q Fever)Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?
- Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?A narrow spectrum antibiotic against Gram –‘s might target the ____________ whereas narrow spectrum drugs against Gram +’s might target the ______________. Select one: a. Cell Wall/Cell Membrane b. Cell Wall/Protein Synthesis c. Cell Membrane/Protein Synthesis d. None of the Above e. Cell Membrane/Cell WallSome bacteria secrete a nonprotein material to cover their antigen. This allows it to go unnoticed by the immune system. That coating is called ___. the capsid the spore cilia the capsule
- A PFU is like a CFU because: it is produced by killing bacteria around a central point. it involves chemicals. it reaches a billion infections and stops. for both, a plate with over 100 countable units is not trustworthy.You extract B cells and dilute the cells so there’s one B cell in each well of a 10-well plate. You then add nothing to 1 of the wells (negative control). To the remaining 9 wells, you add various intact bacterial cells to the wells (a few cells of 1 strain of bacteria per well). After a day, you remove the contents of each of the 10 wells, and examine each of them under a microscope. For the control, you find the one B cell you started with. For 8 of the 9 samples when bacteria were added, you find the one B cell you started with. But for 1 of the 9 samples when bacteria were added, you find 100s of B cells. What does this tell you about the original B cells from the wells where you still only see 1 B cell?You extract B cells and dilute the cells so there’s one B cell in each well of a 10-well plate. You then add nothing to 1 of the wells (negative control). To the remaining 9 wells, you add various intact bacterial cells to the wells (a few cells of 1 strain of bacteria per well). After a day, you remove the contents of each of the 10 wells, and examine each of them under a microscope. For the control, you find the one B cell you started with. For 8 of the 9 samples when bacteria were added, you find the one B cell you started with. But for 1 of the 9 samples when bacteria were added, you find 100s of B cells. What does this tell you about the original B cell from the well where you now see 100s of B cells?
- Describe the structure of the cell wall of a Gram-negative and a Gram-positive bacterium, respectively each cell wall also specify two cell wall components that can be recognized by the constitutive the immune systemYou are working in a medical lab and receive a urine sample from a patient that is displaying urinary tract infection symptoms. You use the urine to set up 8 serial 10-fold dilutions. You decide to spread plate 100 µl of different dilutions. The colony count after incubation overnight: 10-4 dilution has 173 colonies. What is the cfu/ml of this culture?Imagine a microbiologist performed a series of Gram stains but forgot to perform the destain step during the stains. Which type(s) of erroneous results would the scientist observe? Cells that are actually gram-negative would appear gram-positive Cells that are actually gram-positive would appear gram-negative Both gram-negative and gram-positive cells would appear to be the opposite