Which of the following is false about counting viable cells? A. CFU(Colony forming unit) are counted instead of individuals cells B. Only 30-300 CFUs are statistically valid C. Colonies growing on the plate represent a fraction of the live cells in the original sample D. You can determine the number of live cells in a broth culture E. It is necessary to multiply the by CFUs to determine the number of viable cells in the original sample

Which of the following is false about counting viable cells? A. CFU(Colony forming unit) are counted instead of individuals cells B. Only 30-300 CFUs are statistically valid C. Colonies growing on the plate represent a fraction of the live cells in the original sample D. You can determine the number of live cells in a broth culture E. It is necessary to multiply the by CFUs to determine the number of viable cells in the original sample

Complete Textbook Of Phlebotomy

5th Edition

ISBN:9781337464314

Author:Hoeltke

Publisher:Hoeltke

Chapter5: Phlebotomy Equipment

Section: Chapter Questions

Problem 5RQ

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There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one has been incubated for 24 hours. After a 10x dilution by taking 100µl of each culture and adding it to 900 µl water in a microcentrifuge tube and 100µl sample from the tube was taken to view in the counting chamber. a) Count the total number of yeast cells for each culture respectively b) Calculate the concentration and density of yeast cells for each culture respectively
Comparing the different methods for counting bacterial cells, which of the following is FALSE? A. The serial dilution methods counts only viable cells. B. The turbidity method indirectly measures the number of cells observing how much light goes through a broth sample C. The turbidity method uses a spectrophotometer D. The direct microscopic method counts only viable cells E. The direct microscopic method counts both viable and non-viable cells
Increasing the tube voltage on a clinical x-ray unit: a. Improves the resolution of an x-ray image. b. Increases the intensity of the Characteristic x-rays. c. Increases the intensity of the Bremsstrahlung x-rays. d. Increases the energy of the Bremsstrahlung x-rays. e. Increases the energy of the Characteristic x-rays. and why??
You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours. a) How many generations occurred?b) How many cells will be present after the 10 hours?
In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are counted. Answer the question below. there were _____cells in the 1 mL of culture that was spread on the plate. If the culture was diluted 1:100 prior to plating, how many cells were there per mL in the original culture? _________
In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria. Show your work for both.
The following is/are true about plate count EXCEPT a. A plate count is a count of viable or live cells b. Is based on the principle that viable cells replicate and give rise to visible colonies on plates incubated under optimum conditions c. Is based on the assumption that only one cell always lands on a particular spot in the plate and gives rise to one colony d. None of the above Clear my choice
All of the following are valid measurements of growth except: A. Counting viable cells B. Estimating the weight of cells C. Determining the dry weight of cells D. Streaking for single colonies E. Measuring some cellular component F. Counting the total number of cells
You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?
You pipette 1mL from a 30mL starting culture into a 99mL blank labeled A. You plate 1mL and 0.1mL of blank A onto plates B and C, respectively. Plate B has 310 colonies and Plate C has 31 colonies. What is the total cfu of the original culture? Select one: a. 9.3e5 b. 3.1e3 c. 3.1e4 d. 9.3e4 e. 3.1e5
Hi, I have the answer for A, but I am not sure for B and C. For: A, I believe B. B, I think its low colony forming unit count C, I am not sure about it.