Which of the following medium is considered an enriched medium? a. LB b. T-soy  c. Nutrient  d. Chocolate agar plate  d. None of the above

Q: A glass flask is rinsed and autoclaved after use. It was then filled with sterile media in a sterile…

A: Introduction Microbes or microorganisms may be found on almost every surface, in the air we breathe,…

Q: A nurse colleague asks for your help in drawing blood cultures from a patient with suspected…

A: Bacteremia is when bacteria circulate through the bloodstream. It can be caused by everyday habits…

Q: What type of medium is used to maintain and preserve specimens before clinical analysis? a.…

A: Microorganisms are small, unicellular, and microscopic organisms. Any organism capable of causing…

Q: A subculture is a a. colony growing beneath the media surface b. culture made from a contaminant c.…

A: We make subculture from a plate where many different colonies of bacteria are growing.

Q: A fastidious organism must be grown on what type of medium?a. general-purpose mediumb. differential…

A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…

Q: Blood agar media  is a _________.   Selective media Differential media Rich media Enriched media…

A: A medium can be defined as gel or liquid which helps in growth of a bacterial culture. Medium is…

Q: A culture medium on which only Gram-positive organisms grow is called a(n):   Question 9 options:…

A: Culture media is referred to as a media composed of gel or liquid to grow microorganisms. Different…

Q: Does the image above represent a pure culture? Yes O No

A: Answer- No

Q: Viruses were first described as “filterable agents” meaningthat(a) They were often discovered on…

A: Viruses were first described as “filterable agents” meaningt that (c) They would pass through…

Q: The MIC is the________________________________________ of a drug that is required to inhibit growth…

A: There are some living organisms that cannot be observed by the naked eye and only can be seen under…

Q: Explain the rationale behind Macrohematocrit test (Wintrobe method) and Microhematocrit test (Clay…

A: Hematocrit test is used to check the proportion of red blood corposcles (RBC) present. If the RBCs…

Q: One reason that a portion of the soil samples was sifted through an additional sieve was to: F…

A: Related to soil sample test to check for different purposes. like moisture content, air content, the…

Q: MacConkey agar plates represent ________ medium.   a differential a minimal a selective both a…

A: Microorganisms are grown in culture media.  The media used for growth of microbes contain necessary…

Q: Transparent double-sided dishes used for growing microbes are most commonly called A) Petri dishes.…

A: The micro organisms are grown in specific containments for identification of the characteristic…

Q: Milk is usually pasteurized by a. the high-temperature short-time method c. batch method b.…

A: Pasteurization is the process, in which bacteria are killed and the sterilization is done to improve…

Q: Motility is best observed with a a. hanging drop preparation c. streak plate b. negative stain d.…

A: Motility is the ability of bacterial cells to migrate from one location to another. The flagella…

Q: A fastidious organism must be grown on what type of medium?a. general-purpose mediumb. differential…

A: Bacteria require appropriate environmental conditions for their growth. Solid or a liquid…

Q: Which of the following are true of the above picture? (Select all that apply.) V The bacteria are…

A: Gram-negative bacteria have a thin peptidoglycan layer which fails to retain colour after treatment…

Q: Spread plating_is used to sterilize the spreader or Lrod: a. Ethanol bHeating over flame C.…

A: Spread plate technique  is used to isolate bacteria . It is a microbiological technique .

Q: Attached below is the study about the effects of handwashing and the effectiveness of soap against…

A: We only become aware of the significance of handwashing during pandemics. However, its advantages…

Q: Bacteria capable of growing on minimal media are considered which of the following? a. auxotrophs…

A: culture media, also called growth media, are particular mixtures of nutrients and other materials…

Q: MacConkey Agar is considered as:   a. Differential CM only b. Enriched CM only c. Selective CM…

A: MacConkey Agar (MAC) is a selective and differential medium used to isolate and distinguish enteric…

Q: The difference in the colony appearance of bacteria cells growing on mannitol salt agar is detected…

A: Mannitol salt agar is a selective culture media that is used for the isolation of the pathogenic…

Q: Aseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri…

A: Following aseptic techniques is an important part while performing an experiment. Aseptic technique…

Q: An inoculating loop must be flamed to uniform orange-hot from base to tip   A. after culture is…

A: An inoculation loop is a tool used by microbiologists to transfer a small culture of microorganisms.…

Q: A pure culture A) is sterile. B) is a population of identical cells. C) is made of a clearly…

A: These pure cultures are essential for microbiological studies. It facilitates the application of…

Q: C MSA (Mannitol Salt Agar) Match the description to the correct Bacterium A= growth, agar is yellow…

A: Microbiology is the branch of science that deals with the study of microorganisms that are too small…

Q: A pure culture is........... A. A culture of three to four different organisms B. A culture of one…

A: Culture in microbiology is spread of microorganisms in a medium of development . Per body tissue or…

Q: To prepare nutrient agar medium, you have to pour approximately. mL of medium into the Petri plat a.…

A: A patri dish is a normal in which is used for the growth of bacteria and other microorganisms. it is…

Q: The MIC is the of a drug that is required to inhibit growthof a microbe.a. largest concentration b.…

A: Answer: Introduction: MIC stands for Minimum inhibitory concentration. The MIC, or minimum…

Q: The main of objective of using 13-Line streaking method is to get:

A: Answer: 13-Line streaking method: It is the technique of obtaining pure bacterial culture by…

Q: All of the following are valid measurements of growth except: A. Counting viable cells B.…

A: Use the general formula: [Number of colonies counted] × 10 × [how many times the sample must be…

Q: what is the purpose of using oil immersion with the 100x objective lens? a)To prevent refraction of…

A: Answer is option a.) To prevent refraction of light.

Q: Why might gram positive bacteria incorrectly appear to be gram negative after the gram staining…

A: Gram staining is a procedure which is used to identify whether the bacteria being tested in gram…

Q: A trp+, arg+ E. coli cell can grow on? A:Minimal medium B:Minimal medium plus amino acids…

A: There are two different kind of bacteria. They are classified as prototroph and auxotroph based on…

Q: Agar is added to media.

A: Agar is obtained from algae. It is composed of agarose (polysaccharide) and agaropectin. It is a…

Q: Which of the following is used in the sterilization of the medium? a) benmarge b) swab c) autoclave…

A: Sterilization is the process of making something (instrument, swab, room etc. ) free of microbes…

Q: What type of medium is best used to maintain a preserve specimen before clinical analysis? A.…

A: A growth or culture medium is solid, liquid or semi solid in nature that is used to support the…

Q: If a suspect bacterial pathogen infecting your patient is known to be "fastidious" which medium…

A: Fastidious organism : Any organism that requires a particular nutrient in the media is known as a…

Q: Give the use and advantages of the following cultures for the examination of colony characteristics:…

A: Culture media: a. Culture media provides an environment that supplies all essential nutrition for…

Q: 1.0ml of a 1/10

A: As it is well known that for calculating original stock of bacteria we use the formula CFU/ml =(No.…

Q: How do you observe growth in liquid microbiological media? a) the medium is cloudy or turbid b)…

A: Nutrients are required to grow microorganisms invitro i.e outside the body is called culture medium…

Q: Assuming you are working in a microbiology laboratory and a client sent you a sample for the…

A: Microorganisms or microbes are small living organisms (micrometer-sized) that cannot be visualized…

Q: MSA is a selective medium and contains a higher salt concentration, a pH indicator and Mannitol…

A: Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and…

Q: One of the following is INCORRECT about Spread plate a-Allow aerobic bacteria to grow b-The sample…

A: The spread plate method is used to count the viable cells.

Q: ou count 54 colonies of bacteria on an agarp late on which you spread 0.2ml of a 10^-4 (or 1/10,000)…

A: Given information No. of colonies= 54 Volume = 0.2 ml Dilution = 1/10000

Q: Make a chart of all the organisms (Columns) with results    a. Blood Agar Plate b. PEA Agar Plate…

A: As another way of identifying the effects of temperature on the growth of different organisms,…

Q: Transfer of Culture from Agar Plate to Agar Slant

A: The bacterial colonies of interest, after they are isolated from a particular source, need to be…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.