what is the purpose of using oil immersion with the 100x objective lens? a)To prevent refraction of light b)To prevent scratching the slide c)To allow microbes to resuspend in the fluid d)Oil is not used with the 100x, but rather 40x e)To prevent microbes from drying

Q: (a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside…

A: Introduction A bacterial smear is a small layer of bacteria that has been stained on a slide. A…

Q: What is a mixed culture? A contaminated culture?

A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…

Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…

A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…

Q: Why is dilution a necessary part of pure culture preparation?

A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…

Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…

A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…

Q: in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?

A: The Ziehl-Neelson (ZN) stain is the most commonly utilized of the different types of AF staining…

Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…

A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…

Q: eservatives cannot be used for direct + mount ?

A: The disease is a state of the body in which the physiological mechanisms do not function in a normal…

Q: If I was interpreting the results of Durham tubes, Glucose, Lactose, Sucrose, for Staphylococcus…

A: The broth tubes also contain sugars other than glucose such as lactose and mannitol.  Due to a…

Q: Why is it important for a microbiology laboratory technician to use aseptic technique?

A: The majority of the microbes onto which scientists work in laboratories are known pathogens.

Q: shape

A: There are different shapes of bacteria and different classes of bacteria on its shape These shapes…

Q: Why would the optical tweezers be a method superior to dilution and liquid enrichment for obtaining…

A: Optical tweezers have been used to trap dielectric spheres, viruses, bacteria, living cells,…

Q: What are the differences between scanner, low power objective, high power objective and oil…

A:

Q: Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria.…

A: The bacteria cell envelope is a complex multilayered structure and based on this structure the…

Q: What are the applications for Pasteurization, Autoclaving, UV irradiation, and Gamma irradiation?

A: It is required to mention the application of Pasteurization, Autoclaving, UV irradiation, and Gamma…

Q: Why is a blank used in the spectrophotometer before the actual pigment is tested?

A: spectrophotometer is a device used in spectral analytical techniques like UV spectroscopy , in which…

Q: What are the advantages of UV disinfection versus, or as acomplement to, chemical disinfection with…

A: UV disinfection treatment is a very rapid physical process for disinfecting or sterilizing any…

Q: How is the correct way of incubating culture plate media? Explain why is that so.

A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…

Q: What advantages do solid media offer for the isolation of microorganisms?

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable…

A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…

Q: Why is it necessary to use immersion oil when the 100X objective is used'

A: Note - we answer one question at a time. Immersion oil is transparent and has specific viscosity and…

Q: Is the autoclaving process absolute in killing all microorganisms? If not, what are the factors that…

A: Autoclave is an instrument or device which is used in  method of moist heat sterilisation; while…

Q: The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope…

A: Ans: Lugols solution: The Lugols solution is 2.5 to 25% weight by volume total iodine.

Q: The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a…

A: The technique is streak plate technique. Streak plate method is a pure culture technique which helps…

Q: What is one advantage of a pour plate over a streak plate?

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: Of what practical importance are air borne microorganisms to the laboratory workers?  What…

A: Questions related to the microbiology labs.  Note: each questions are answered under particular…

Q: How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other…

A: Bacillus subtilis is a very well bacteria that is widely used as a Gram-positive bacteria model…

Q: What is the purpose of adding sodium bicarbonate to a culture medium?

A: >> The purpose of adding sodium bicarbonate to animal culture medium is that it acts as a…

Q: What is the main advantage of using the slide culture technique for identifying molds?

A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…

Q: n is 100x, what is the magnification of n is 40x, what is the magnification of t gnification is 10x,…

A: In light microscopy, there are several different types of microscopes, but the four most common are…

Q: Are TSA, MRS and MOX differential media? Why or why not?

A: In microbial biology, differential media refers to any growth media that comprises of certain…

Q: Can hand sanitizer kill bacteria?

A: Sanitizer is referred to as any substance or fluid that is used to prevent the spread of pathogens…

Q: How can moist heat sterilization can sterilize antibiotic solution?

A: Moist heat sterilization is a technique that uses heated, increased pressure steam to sterilize an…

Q: If a specimen is being viewed under a 40x objective, and the microscope has a 10x ocular lens, what…

A: Magnification is defined as a measure of the ability of a lens or other optical instruments to…

Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…

A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…

Q: What are the advantanges and disadvantages of using stained preparations for observing microbial…

A: Characteristics of microbes are studied using a microscope by microbiologists. Some microbes like…

Q: What is the differences between ocular magnification and objective magnification?

A: Introduction  A microscope's objective lens is the one near the sample at the bottom. It's an…

Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?

A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…

Q: agnification is required to observe microorganisms?

A: A microorganism, often known as a microbe, is a microscopic organism that can be a single cell or a…

Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…

A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.

Q: do we need to stain microorganisms?

A: The study of microorganisms is termed microbiology. Microorganisms is microorganism, fungi, archaea…

Q: why is it necessary to use oil immersion on oil objective ?

A: The oil immersion is the technique of light microscopy, which is used to increase the resolving…

Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…

A:

Q: What is the main difference between Digital Mammography and Conventional Mammography?

A: A diagnostic tool is an instrument or a device used to diagnose the health status of a body. This…

Q: What are the immersion media that need to be used?

A: Immersion media is used to increase the numerical aperture of the microscope by a factor equal to…

Q: What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?

A: Spectroscopy is a procedure utilized for the evaluation, portrayal, and basic investigation of a…

Q: To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a…

A: Given Values: Volume of the 16 hour culture = 500 μl Final Volume of the culture media = 5 ml