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The main of objective of using 13-Line streaking method is to get:
A-Contaminated cultures
B-None of the above
C-Overlapped colonies
D-Separated colonies
Step by step
Solved in 2 steps
- What are the basic differences between the three plating methods? Fill up the following table. Streak Pour Spread Equipment/ Materials used in immobilizing and separating cells Purpose(s) (isolation, enumeration, both) Type of colonies (surface, subsurface, both) Advantage DisadvantageWhat method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line inoculation with a loop C. Streak plate with a loop D. Spread plate with a glass spreaderA subculture is a a. colony growing beneath the media surface b. culture made from a contaminant c. culture made in an embryo d. culture made from an isolated colony
- You have performed an experiment and your aim is to obtain pure colonies. However, you observe some colony variations and you need to verify that this is not due to contamination, name and describe the staining method would you use to achieve this goal?A culture medium that does not grow gram-positive organisms would be a differential plate enrichment plate selective plate streak plate pour plateA pure culture is........... A. A culture of three to four different organisms B. A culture of one organism and another C. A culture if different organisms D. A culture of one organism
- a pure bacterial culture was diluted by adding a 0.2 mL aliquot to 0.9mL water. Then 0.1 mL of this dilution was plated out, yielding 82 colonies. Calculate the CFU/mL in the original culture.A fastidious organism must be grown on what type of medium?a. general-purpose mediumb. differential mediumc. defi ned mediumd. enriched mediumA culture of E. coli has a concentration of 5 x 108cells/mL. How many times do you have todilute the culture so that when you spread 0.1mL on an agar plate you will have 250 colonies?Hint: you need to find dtotal and then convert it into a DF.
- Which of the following is not meant to reduce contamination of yoru sample? avoiding hand contact with the speciman none of these heating the inoculation loop until red heat fixation of the speciman flaming the lip of a test tube cultureA capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C Simple Stain D Differential stain E Negative StainWhy is it important to use a sterilized loop between streaks when preparing a streakplate? Observation of a streakplate culture shows more growth in Quadrant 4 than in Quadrant 3. Account for this observation. Describe the way in which you can isolate an individual colony from a spreadplate or a streakplate that holds multiple colonies. Outline the differences between a streak plate and a spread plate.