Which of the following reagents will you include in the blank (negative control) for this experiment? 00000 Alkaline phosphatase Buffer solution: 50mM Tris-HCl, pH=10 Vitamin C Acid Molybdate Distilled water Rhino horn
Which of the following reagents will you include in the blank (negative control) for this experiment? 00000 Alkaline phosphatase Buffer solution: 50mM Tris-HCl, pH=10 Vitamin C Acid Molybdate Distilled water Rhino horn
Chapter6: Oral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 9.5P
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Study the following experimental method:
• Enzyme solution: Alkaline phosphatase in 50mM Tris-HCl, pH=10
Buffer solution: 50mM Tris-HCl, pH=10
• Vitamin C
• Acid Molybdate
Method
Prepare the 2 following test tubes:
Add 100μl of the enzyme solution to 500μl of the horn sample. Add 2.4ml of the buffer solution and mix the solution.
Add 100μl of the buffer solution to 500μl of the horn sample. Add 2.4ml of the buffer solution and mix the solution.
Add 1ml Vitamin C, 1ml Acid Molybdate and 5ml water to the 2 tubes and mix.
Leave the 2 tubes at room temperature for 20 minutes and read the absorbance at 670nm.
a.
b.
Determination of the organophosphate concentration of a horn sample.
A standard curve of phosphate will be provided.
Determine the concentration of the organophosphate in the horn sample.
Which of the following reagents will you include in the blank (negative control) for this experiment?
Alkaline phosphatase
O Buffer solution: 50mM Tris-HCl, pH=10
Vitamin C
Acid Molybdate
Distilled water
Rhino horn
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