Materials: • Single colony of E. coli cells to be transformed • LB medium • 0.1 M CaCl2, ice cold (How to prepare?) • LB amp plates 42 °C water bath • 0.1 M CaCl2+15% glycerol, sterile (How to prepare?)
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- Results from a Kirby Bauer antibiotic assay on a Gram-negative bacterial culture are described as follows: A) the bacterium is resistant to penicillin, an antibiotic that targets synthesis of the peptidoglycan cell wall and B) the bacterium is resistant to tetracycline, an antibiotic that targets the small subunit of the ribosome, inhibiting protein synthesis. Which of the results represents intrinsic resistant and which represents acquired resistants?Explain about different types of bioreactors and how they are used in cell suspension culture. What are the advantage and limitations of each bioreactors?1.Define Bioprospecting.2. What properties in microorganisms were researchers at SINTEF and NTNU looking for?3. What made them select Micrococcus luteus as a candidate organism? 4. Describe the steps involved in genetic engineering, prior to commercial production of the potential `UVAblue’ sunscreen.5. What damage do long wave UV radiations induce in human cells?
- is bradford assay using dye Coomassie Brilliant Blue G-250 a different method from using a spectrophotometer ? if so how is it different to determine protein concentration?Environmental biosensors are analytical devices and the following is a diagram of the structure of environmental biosensors. Briefly describe the working principle of both direct and indirect assays of biosensor taking consideration of any one of the available examples as biological recognition element and physical transducer.Bio If you plated 100 uL of bacteria from a 10-5 dilution and count 73 colonies after incubating for 24hrs, what is the CFU/mL of the original (undiluted) culture? Question 3 options: 7.3 7.3 x 10^7 7.3 x 10^-6 0.73
- For each mode of bioreactor operation listed below, name one advantage and one disadvantage. a. Batch b. Fed-batch c. ContinuousGive 2 fluorogenic methods that can be used in detecting coliforms and E.coli.With detail, compare and contrast the following 5 real-time assays; Taqman, SYBR Green, Molecular Beacon, FRET, and Scorpion. Please write complete sentences in paragraph form. Identify how the assays are similar but more importantly, what are the distinguishing features of each of the technologies.
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