Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria. If you omitted the iodine (mordant) step in the procedure, what colors would the bacteria on your slide appear to be?
Q: Consider, for example, that a particular serine residue is phosphorylated to activate the protein.…
A: Phosphorylation can either activate or deactivate a protein. Phosphomimetics replaces amino acids in…
Q: (a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin method…
A: Absorbance of a solution is directly proportional to its concentration. This is as per Lambert Beer…
Q: Examine the structures of the following molecules. In each compound circle and identify by name each…
A: The answer is given in the image.
Q: What biomolecule contains the genetic information of SARS-CoV-2? Be specific. -Describe the…
A: SARS-CoV-2 is an infectious virus that is responsible for the recent COVID pandemic. It is a…
Q: A) Is this reaction ( in picture provided) in equilibrium? B) If it is not then ,what is ∆G' at…
A: Gibbs free energy change, equilibrium constant and reaction quotients are values for a reaction…
Q: It is an essential amino acid with a nonpolar side chain O arginine O proline tryptophan O threonine…
A: Aminoacids are the building blocks of proteins with polar and non polar side chains. Amino acids are…
Q: Give are examples of nucleic acid in food and sources of dietary nucleotides?
A: Nucleic acid in food and sources of dietary nucleotides
Q: ATP Accounting Upon digestion of strach, isomaltose, one of its degradation products is further…
A: Carbohydrates are principle fuel in the body. Most of the energy is derived from the digestion of…
Q: Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains…
A: Sickle cell anemia is a result of a mutation in the HBB gene. This gene contains the restriction…
Q: would likely be in the center of would likely be associated with the polar head groups and In an…
A: The amino acids are classified as polar and non-polar. The integral membrane proteins are…
Q: what are the uses of nucleic acids? a-Three things about how nucleic acids are used in cells:…
A: The nucleic acid polymer has nucleotide as its monomeric unit. synthesis of nucleotides is an…
Q: Mention four advantages and two limitations of capillary electrophoresis over HPLC.
A: Capillary Electrophoresis (CE) is a widely used separation technique in biochemistry. Here a very…
Q: Photodynamic therapy results in induction of WAF1 or CIP1 or P21 leading to cell cycle arrest and…
A: Photodynamic therapy is a therapy involving use of a drug called photosensitizer which got activated…
Q: Which of these statements is INCORRECT? O Glutamic acid and asparagine are acidic amino acids…
A: Amino acids are biomolecules that have an amino group and a carboxyl group linked to the same carbon…
Q: QUESTION 14 Match pairs of terms together that have the same value b. 1 micromole 1 μmole/l 1 mM 1…
A: 0.1 = 10-1 (deci)0.01 = 10-2 (centi) 0.001 = 10-3 (milli) 0.000 001 = 10-6 (micro)0.000 000 001 =…
Q: Question 2 HER2 and HER3 belong to a family of receptor tyrosine kinases (RTKS). A representative…
A: The Human Epidermal Growth Factor Receptor (HER) or simply Epidermal Growth Factor Receptor (EGFR)…
Q: Che mi c al Reaction water+ reactants enzymes 42. If the reactants are lipids, then the products are…
A: Since the second question is an incomplete question because one of the options is cropped, we will…
Q: Posttranslational modifications of proteins do not include: peptide bond formation glycosylation…
A: Proteins are one of the essential biomolecules for life. These are formed by translation from the…
Q: Below is a list of hypothetical Hemoglobin variants. Circle the name of the variant that is most…
A: Hemoglobin exists in the T and R state during the absence and presence of oxygen respectively. In…
Q: 1. Classify the 20 common amino acids C. According to Nutritional Requirements g. Non-essential h.…
A: Amino acids are biomolecules that have an amino group and a carboxyl group attached to the same…
Q: Explain how the lipid bilayer modulate the activity of proteins. Give 2 examples.
A: Lipid bilayer is a bilayer of different types of phospholipids. Plasma membrane is a lipid bilayer…
Q: Q4. The estimated gKa of hydroimidazolone's R group is 6.93. If the pK₁ (a-COOH) and pK₂ (a-NH₂) of…
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: The initial step in the protein translation is described by: O release of the initiation factors…
A: There are 4 Biomacromolecules. They are ; Proteins Nucleic acids Carbohydrates and Lipids
Q: why the human dna is considered as a fibonacci sequence?
A: A Fibonacci Sequence is where each number is the sum of two preceding numbers, like…
Q: C. The dissociation constant, (Kdisso) for the ES complex
A: Option c is the answer
Q: Starting as a lipid in some holiday prime rib, trace the path that energy and biomass make as that…
A: It is well known to us all that both mass and energy are conserved in the universe. Hence all mass…
Q: UDP- glucose 12. pathway glycogen ATP+ HCO amino acid 9. metabolite glu 'NH. & metabolite ornithine…
A: The pathways shown in the image represents integration of metabolism which showcase that all the…
Q: Which set of amino acids will likely to be located on the interior of a globular protei O Lys, Phe,…
A: Aminoacids are the building blocks of proteins. They are classified into polar and non polar amino…
Q: The most abundant substance in living systems is: U O proteins DNA water O glucose Question 46 Which…
A: Life exists on the earth is the chemical ingridents like water and carbon. Air and water are the two…
Q: Which of the following is incorrect? a. Regulatory steps are irreversible O b. Regulation of a…
A: Regulatory steps are the important steps in a metabolic pathway which are slowest and they can be…
Q: H₂N. OH DCC Reactive Intermediate + H₂N.
A: Amino acids are biomolecules that contain an amino group and a carboxyl group linked to the alpha…
Q: Activity 2: Determine the volume from micropipette reading Write down the correct volume for each…
A: Micropipette are the tools used for measuring, transfering a very fine volume in the range of…
Q: Ok, thank you so much. In case of Km, we cant get the value? Do we just mention that 2.085 on x-axis…
A: Km is the michaelis menton constant which is nothing but substrate concentration half of maximum…
Q: The figure below shows the structure of a porin subunit embedded in the lipid bilayer (left: side…
A: A transmembrane protein is a protein that traverses through the membrane entirely from one side to…
Q: I have a question When they ask in an exam question "draw two pyranose form of D-glucose, what do…
A: Pyranose form of sugar refers to the six membered ring structure that consists give carbon atoms and…
Q: Which of the following is incorrect about the beta sheet? Parallel beta sheets are less stable than…
A: In a protein the polypeptide chains have four levels of structural organization and they are…
Q: In nucleic Acid Draw/ Illustrate the structures of purines and pyrimidines.
A: Nucleic acids are the polymer of nucleotides. There are two types of nucleotides: de-oxyribo…
Q: At neutral pH (pH = 7): a. acidic amino acids have a net positive charge b. basic amino acids…
A: Net charge of an amino acid is determined based on the PH of the medium and pKa of the amino group,…
Q: Without checking the accuracy of a given Pipetman, would you pre- dict that it is better to use a…
A: Micropipette are the tools used for measuring, transfering a very fine volume in the range of…
Q: Table 1: Absorbance data @ 500nm for Glucose standards (0-20mM) and tests solutions Tube Number…
A: Standard curve for glucose concentration is constructed by taking concentration of glucose on x axis…
Q: Answer for Number 2 question. Thank you. no need for a long explanation.
A: Group of cells called glands. There are 2 types of glands exocrine glands and endocrine glands…
Q: Why is oxytocin sometimes administered to a woman following parturition?
A: Oxytocin is a hormone synthesized in the hypothalamus, and carried to the posterior pituitary gland.…
Q: Which of the following statements about enzymes are true? Choose all that apply. Group of answer…
A: The enzymes are biological catalysts that enhances the rates of biological enzymes. Most enzymes are…
Q: What distinguishes metalloproteins from glycoproteins in particular?
A: Some proteins contain one or more chemical groups(other than amino acids) covalently linked to them.…
Q: functional groups in palmitic acid, a saturated fatty acid and oleic acid, an unsaturated fatty acid
A: Biological lipids are a chemically diverse group of organic compounds which are insoluble or…
Q: Define the terms (i) facilitated diffusion, (ii) primary active transport and (iii) secondary active…
A: Transport means the export and import of molecules inside the cell or outside the cell. The membrane…
Q: You are given two samples of DNA, one from Clostridium perfringens and the other from Mycobacterium…
A: DNA is a nucleic acid that is the genetic material for most organisms (except some virus). It…
Q: The above reaction coordinate diagram in the presence of enzyme is inconsistent with the "lock and…
A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…
Q: Which technique is used to characterize multiprotein complexes ?
A: Multiprotein complexes are termed as MPCs that are important for cell signalling. Characterisation…
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- If you gram stain an acid fast organism, what would you most likely see when you examine the stain under oil immersion? You are in a hurry to test a bacterial culture for spore production. You grow the culture or 12 hours and then stain it. Your results are negative. Should you trust your results? Why or why not?Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed on slide and subjected to Gram staining. What do you observe under the microscope when the following steps are skipped? Please explain.a. Staining with safraninb. Staining with Gram iodinec. DecolorizationAfter conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in your slide under the microscope, what could be the mistake you have done? What can you observe when you view a Gram stained-mixture of bacterial specimen?
- Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram negative bacteria. After finishing, she realizes she mixed up the safranin and alcohol steps. What is likely to be observed when she views the slide under the microscope? All cells will appear purple, regardless of whether they are actually Gram negative or Gram positive. All cells will appear pink, regardless of whether they are actually Gram negative or Gram positive. Gram negative cells will be clear, Gram positive cells will be purple. Gram positive cells will be clear, Gram negative cells will be pink. Cells will appear clear, regardless of whether they are actually Gram negative or Gram positive.You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? a)red/pink b)purple c)colorlessWhy is it important to perform a gram stain for any type of bacteria? After performing the gram stain method what would be the next step? (Think in terms of the real-world situation) Explain.
- You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? Red/Pink Purple colorlessWhen performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. The alcohol wash removes crystal violet and iodine from the bacteria. Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.Suppose you are staining three identical smears of the same bacterial culture with three different basic simple stains: carbol fuschin, crystal violet and methylene blue. You observe each stained preparation under oil immersion at 100X. which of the following bacterial characteristics would you expect to be different between each of the stains as you view them under the microscope. Bacterial arrangement Bacterial shape Size of cells The color of the bacteria
- When doing a gram stain in microbiology, one step can be eliminated and still allow distinction between gram-positive and gram-negative organisms. What is the step and why would eliminating it still result in the ability to distinguish gram-positive from gram-negative?After performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explainwhy, the result you would want to observe and the interpretation of the result.Why do you suppose endospore-forming bacteria are commonly found in the soil? Suppose your unknown bacteria is a Gram-negative rod. You decide to perform an endospore stain, thinking it will help you with the identification of the organism. What will you see, and why?