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Name: Which of the following would you expect to act only in cis (and not in
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Description: Which of the following would you expect to act only in cis (and not in trans)? Select all correct answers.A.) lac repressorB.)trp operon antiterminatorC.)CRPD.)CRP-binding siteE.)lac operator

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Biology

Which of the following would you expect to act only in cis (and not in trans)? Select all correct answers. A.) lac repressor B.)trp operon antiterminator C.)CRP D.)CRP-binding site E.)lac operator

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

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A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcription
If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
If you add a co-eepressor to a repressor protein and that allows it to bind the operator is this a inducible, constitutive or repressible operon?
What led researchers to conclude that a repressor moleculeregulates the lac operon?
How do we know that the trp operon is a repressible controlsystem, in contrast to the lac operon, which is an induciblecontrol system?
If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
Can you think for how long the lack operon would be expreesed in the presence of lactose?
If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or glucose, how many proteins (and which ones) are bound to the lac operon? b. Without lactose, but with glucose, how many proteins (and which ones) are bound to the lac operon??
A mutation occurs in the 5′ UTR of the trp operon that reduces the ability of region 2 to pair with region 3. What will the effect of this mutation be when the tryptophan level is high? When the tryptophan level is low?
What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)
According to the operon model, for the synthesis of an inducible enzyme to occur, the end-product must not be in excess an inducer must bind to the enzyme an inducer must bind to the repressor repressor must bind to the operator repressor must not be synthesized
Let’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively expressed. In other words, the operon is turned on in the presence or absence of lactose. One possibility is that the mutation mayblock the transcription of the lacI gene, thereby preventing the synthesis of lac repressor. A second possibility is that the mutation could alter the sequence of the lac operator in a way that prevents lac repressor from binding to the operator. How would you distinguish between these two possibilities?