Which ONE of the following mobile phases would give the SHORTEST retention time of prednisolone using a reverse-phase column? HO. но. HO H. Prednisolone
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- Briefly outline why cinnamaldehyde, cinnamic acid and cinnamyl alcohol travelled different distances on the TLC plate. The solution used to develop the TLC plate was a mixture of toluence and methanol (8:1.5)Principles of separation by capillary IsolachophoresisDoe hydrophobic silica gel act as non-polar stationary phase in TLC making the process as reverse chromatography? explain
- Which two of compounds below will be difficult to be separated by column chromatography (using ethanol as solvent) and why? -p-anisic acid -methyl-4-aminobenzoate -benzoic acid -ethyl-3-hydroxybenzoate -ethyl-4-nitrobenzoate -trans-cinnamic acid -benzanilide -2-methyl-1-nitronapthalene -9-fluorenone -naphthalene -trans-stilbene -vanillin2-pentanone has a retention index of 987 on a poly(ethylene glycol) column (also called Carbowax). (a) Between which two straight-chain hydrocarbons is 2-pentanone eluted? (b) An unretained solute is eluted from a certain column in 1.80 min. Decane (C10H22) is eluted in 15.63 min and undecane (C11H24) is eluted in 17.22 min. What is the retention time of a compound whose retention index is 1 050?If a 0.0920 g sample of a 1:1 mixture of 4-nitroaniline and 2-nitroaniline was separated by column chromatography, and the recovered fractions weighed 0.0191 g ( 4-nitroaniline) and 0.0200 g (2-nitroaniline), what was the percent recovery of these two isomers? 20.6% 43.5% 21.7% 42.4%
- Identify these statements with true or false true or false I. Chromatography utilizes partition coefficient theory true or false II. In reverse phase chromatography, the mobile phase is a polar solvent true or false I. Aspirin is an ester prodrug. true or false Aspirin is synthesized by means of an acid catalyzed reaction. true or false I. Grain alcohol boils at 78.3 C true or false Wood alcohol boils at 60 C true or false Diazo coupling involves a sigma and a pi bond in two nitrogen atoms true or false When methyl orange passes through from basic to acidic medium, the change in color is from yellow to intense red true or false Benzoic acid is a white crystalline substance that has a melting point of 122 C true or false Benzoic acid can be prepared by the alkaline reduction of toluene true or false Potassium permanganate is an oxidizing agent true or false Potassium permanganate should never be mixed with glycerin true or false Methyl…Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spotswere detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. If the TLC chromatogram in the picture was duplicated but using a column, draw the set-up and label approximate spots.The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67
- In reversed phase partition chromatography Select one: a. the least polar compound elutes first b. the smallest molecular weight compound elutes first c. the most polar compound elutes first d. the largest molecular weight compound elutes first e. the lowest boiling compound elutes first f. the highest boiling compound elutes firstTell me about the Laboratory experiment: Purifying Protein by Column Chromatography Thank you^^A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most soluble in the mobile phase and why?