Which sensor, when it comes to sensitivity, will be favoured as a biosensor, the one with great sensitivity or the one with low sensitivity? Justify your position using examples.
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Which sensor, when it comes to sensitivity, will be favoured as a biosensor, the one with great sensitivity or the one with low sensitivity? Justify your position using examples.
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- Sketch how an ion-selective field effecttransistor (ISFET) can be used as a biosensor to detect DNA in solution,detailing the mechanism for sensing. Explain why the use of a SiNW could leadto increased sensitivity?When it comes to the sensitivityof the sensors, which one will bemore preferred asa biosensor,the one with high sensitivity orwith the low sensitivity ? Explainthe reason with examples.When bound to proteins, the Bradford reagent turns blue. You measured the absorbance of your samples at 595 nm, which corresponds to yellow light. Why did you measure the absorbance using yellow light instead of blue light?
- Environmental biosensors are analytical devices and the following is a diagram of the structure of environmental biosensors. Briefly describe the working principle of both direct and indirect assays of biosensor taking consideration of any one of the available examples as biological recognition element and physical transducer.Mention assays other than biuret and lowry assays that are used to detect the presence of proteins. Talk about the principle, the advantages, and the disadvantages.What phi and psi angles would you predict to be most prevalent for myoglobin and green fluorescent protein, in a Ramachandran plot
- In the assay of citric acid, what do you think about the nature of solution sample ? a) Before titration and b) After titration.When you graph your protein assay data in Excel, should you include the absorbance of your unknown BSA sample? Explain why or why notThe Bradford reagent gives a linear response only from 0.1 mg/mL to 1.4 mg/mL of protein concentration. Would we be able to use an "absorbance vs. concentration" line graph for samples outside of the range indicated? Justify your response.
- Why are buffers important in biochemical experiments?Suppose you turn on your power supply to run the gel and find that the milliamps reading is close to zero. What would you check?Describe the effects of a lethal dose of ionizingradiation at the molecular level. What type of filterwould be used to filter sterilize a heat-sensitive liquid?