Negatively charged proteins migrate towards the top of the gel backwards towards the positive electrode O slower than positively charged proteins
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- An amino acid mixture consisting of phenylalanine, glycine, and glutamic acid is to be separated by HPLC. The stationary phase is aqueous and the mobile phase is a solvent less polar than water. Which of these amino acids will move the fastest? Which one will move the slowest?Solutes tend to diffuse from a region where they are _______ concentrated to an adjacent region where they are _______ concentrated. a. more, less b. less, more c. movement is independent of concentrationTrue or false Hydrophobic talis of detergent molecules can bind to hydrophobic regions of integral membrane proteins, extracting them from the membrane by formingdetergent protein complexes that are soluble in aqueous solution
- You had a second solution with an unknown concentration of Protein X that had to be diluted 4x before you could measure the absorbance. The diluted solution had an absorbance of 0.76. What is the concentration of the diluted solution in ug/ml and what is the concentration of the original solutionThe mobility of integral proteins can be measured by physical state of the ______________a) amino acidsb) external phospholipidsc) membrane phospholipidsd) membrane appendagesEnzyme elastase cleaves polypeptides after ______________ aa. small Positively charged Negatively charged Large, non-polar
- You have a mixture of 4 amino acids (Q, W, E, R, T) that you want to separate using ion exchange chromatography. Which of these amino acids would bind most strongly to a DEAE anion exchange column at pH 7? Q W E R TA red blood cell (300 mOsm/L) is placed in a solution of 100 mM NaCl + 100 mM glucose. Is the solution best described as Hypotonic, Isotonic or Hypertonic? Explain your answerIs phosphate H2PO4 non polar or polar
- A large chaperone protein complex GroEL is approximately 16 nm in diameter. When it is dissolved in water at 300 K, estimate the average time it will take for GroEL to diffuse a distance of 500 nm (0.5 micron). The viscosity of water is 10-3 Pa*s.Hoechst 33342 is a membrane-permeant dye thatfluoresces when it binds to DNA. When a population ofcells is incubated briefly with Hoechst dye and then sortedin a flow cytometer, which measures the fluorescence ofeach cell, the cells display various levels of fluorescence asshown in Figure Q17–1.Mild, non-ionic detergents (like Triton X-100, with polar but uncharged regions that do not denatureproteins) would be required for separation of which of the following proteins from cell membranes?A. monolayer-associated proteinsB. lipid-linked proteinsC. transmembrane proteinsD. integral proteinsE. peripheral proteins