Which sequencing project(s) would be done better using a next generation method (like Illumina) instead of Sanger sequencing? Choose one or more. Sequence the gene encoding the SARS COV-2 spike protein Sequence the genome of a newly isolated bacterium Sequence the genome of new isolate of the monkeypox virus (we know the sequence of some monkeypox isolates already)
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- Give typed full explanation Suppose a nucleic acid amplification test (for example, a PCR test performed to diagnosis a virus) gives a negative result - what would a "negative" PCR test result specifically mean? Group of answer choices the individual tested was disease-free no DNA amplification was observed no viral DNA was present in the host the target DNA sequence was replicated no RNA was present in the sample testedCRISPR is a tool used by bacteria to fight virus attacks. In your own words, describe how CRISPR works in bacterial systems, and how this idea can be used for genome editing.What molecule is next generation sequencing analyzing? DNA, RNA, or protein.
- Draw an annotated diagram outlining the steps involved in a Next Generation sequencing technology of your choice (NOT capillary electrophoresis)You need to complete the following steps for an experiment. Explain whether an acrylamide gel or an agarose gel is more appropriate to use for the following experiments. Purifying tRNA from total RNA Isolating a vector insert for cloning Analyzing PCR productsYour next task is to study Ebola virus (EboV) sequences of different strains. You have received sequences of different Ebola virus strains collected during the Ebola outbreak of 2013 – 2016. EboV from Guinea pig Reference DNA Sample CTA TGC AAG CAG TTA mRNA Protein
- Give a lab report introduction. introduction should include a general background on bacteriophages, the use of bacteriophages in bacterial genome engineering, and a description of the overall practical aims. Total word count should be no greater than 500 words.What are three benefits Ion torrent sequencing has over sanger sequencingChemistry Biochemistry: Polymerase Chain Reaction Please compare and contrast these three OR make table: 1.qPCR 2. RT-PCR 3. PCR
- NGS sequencing made it easy to generate COVID-19 sequences and comparethem at different time points. Using what you learn in the course you are able to analyzeCOVID-19 data at different waves.Requirements1. Use the GISAID database (GISAID) to download COVID-19 sequences (at leastten samples) at different time points.2. Perform multiple sequence alignment for your samples.3. Generate a phylogenetic tree and visualize it.4. Use BLAST to align COVID-19 spike protein with other viral spike proteins.5. Choose the most homologous protein and annotate it using ensemble or anypreferred database.6. All analysis steps must be delivered in bash scriptGive three advantages ‘Next Gen sequencing’ has over Sanger sequencing for clinical microbiology.What is the program Finch TV used for? Viewing next generation sequencing data Opening sequencing files with the .bam file extension Opening sanger sequencing files with the .ab1 file extension Opening NGS files with the .ab1 file extension