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- A fresh TLC plate is spotted with compound A,B and C, but with methylene chloride as a solvent. How does the change in solvent system affect the Rf value of compound A?A thin layer chromatography experiment is planned to determine the Rf values of four compounds. List the following compounds in order of increasing Rf values from shortest to longest. (If B is shortest, then A, C, and D is longest, enter BACD )For Fluorenonce and benzoic acid 1. Which compound would you expect to be the top spot on the TLC and why? 2. Which compound would you expect to be the first to come off the column and why? 3. What data supports the identification of the compound in a yellow fraction?
- Compound A had an Rf value of 0.35 in pentane and 0.45 in diethyl ether when generated on a TLC plate using pentane. Compound B exhibits Rf 0.42 in pentane and 0.60 in diethyl ether. What solvent should be used for the TLC separation of an A/B mixture? Explain.An unknown mixture was analyzed with TLC on a silica gel plate. Development gave two spots with Rf values of 1.0 and 0.0 respectively.a) Can you say for sure how many compounds are present in the mixture? If so, how many are there? Explain. b) If not, what additional TLC experiment(s) might be performed to determine how many components are present? Explain.When naphthalene, butyric acid, and phenyl acetate are separated using dichloromethane as the developing solvent on a silica gel TLC plate, which compound would have the highest Rf value? Which would have the lowest?
- Why mixture of solvents such as ethyl acetate with 5% acetic acid is used as a solvent/mobile phase for developing TLC? Please explain in detail.I had having trouble understanding the TLC labs. Could someone help explain how to tell which compound has a higher Rf value? benzoic acid or trans-stilbene?1. a compound has an Rf value of 0.97 diethyl ether but an Rf value of 0.045 in cyclohexane. Suggest a couple (at least three) solvent (solvents), or solvent system (systems), that might be better suited for this particular compound. 2. suppose your TLC plate shows only one spot at an Rf value of 0.015 (essentially it does not move). Does this mean that your sample is 100% pure? Explain your answer. 3. suppose your TLC plate shows only one spot at an Rf value of 0.98 (essentially with the solvent front). Does this mean that your sample is 100% pure? Explain your answer.
- In a gas chromatogram, the retention time of compounds A and B are 1.25 min and 1.45 min, respectively. If both of these compounds have similar structures, which of the compounds has a higher boiling point?How would you discuss method of separation and identification of compounds in TLC?Identify the statement that correctly interprets this TLC analysis of a recrystallized sample of acetaminophen produced following the procedure outlined in Experiment 5: The TLC of an individuals purified acetaminophen showed two spots. One spot had an Rf identical to that of pure acetaminophen. This spot was also larger and darker than the second spot. The Rf of the second spot was very close to the Rf of pure 4-aminophenol. a) The two spots evident in my TLC analysis indicate my sample is a mixture. Since each spot has an Rf value that is close to the Rf of one of the authentic materials tested, I can conclude my sample is a mixture of acetaminophen and 4-aminophenol. The large size of the spot with an Rf nearly identical to authentic acetaminophen indicates my sample is mostly 4-aminophenol. b)I can conclude from this evidence that my sample is a mixture of acetaminophen and 4-aminophenol because the Rf of each of the spots is nearly identical to one of the authentic…