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AUTOIMMUNE DISEASE ASSAYS ANA (AntiNuclear Antibody)
- Why are ANAs produced by the body?
- How are these ANAs produced by the body?
- Why is the homogeneous pattern most common in SLE?
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Solved in 4 steps
- AUTOIMMUNE DISEASE ASSAYS ANA (Anti-Nuclear Antibody) What other methods are used to test for presence of ANAs? Give the principle of each. How does each of the listed methods differ from the one performed in the lab?Define the principle immunoturbidimetric C3 assaydiscussion: use evidence. Describe donor selection and blood processing, including assays for bloodborne infectious diseases. Describe the means of detecting antigen-antibody reactions in transfusion medicine, including the role of complement. Discuss the preparation and requirements of antisera.
- Different MHC I molecules between donor and recipient cells can lead to rejection of a transplanted organ or tissue. Suggest a reason for this.Describe the cancer immunotherapy that won the Nobel Prize in 2018. Please include: how they discovered the mechanism of action describe the mechanism of action what drug did they developedCold-Reacting Autoantibodies. Most adult sera contain low titers of cold-reacting autoantibodies, most notably autoanti-I, autoanti-H, and autoanti-IH. These antibodies are usually IgM and of no clinical significance. They are troublesome in that they may interfere with the detection of significant antibodies, resulting in prolonged workups and delayed transfusions.Cold-reacting autoantibodies may be suspected when the screen cells, panel cells, and the autocontrol areall positive at the immediate spin phase and reactivity gets weaker or disappears with incubation at 37°C (Fig. 9–18).Certain cold autoantibodies activate complement and may be detected at the AHG phase when using complement-containing AHG reagent. These autoanti- bodies may be mistaken for weakly reacting IgG antibodies.Although it is not usually necessary to determine the specificity of the cold autoantibody, testing against additional cells may confirm its presence. Cord blood cells that lack the I antigen are of…
- What are the differences between “O” and “H” antigen? Differentiate Widal from Weil-Felix test. The strains of Proteus vulgaris are used in Weil-felix reaction. Explain why.Serum from individuals with high levels of antibody to SARS-CoV2 has been used to treat patients with severe COVID-19. What is ONE way (there are several) that passive immunization with the antibody to the virus could help these patients? HINT: think about what opsonization with antibody could do for the innate immune response.Why monoclonal antibodies method is better than Polyclonal Antibodies method in detecting specific pathogen. Explain reasons.
- Serological testing:b. How would one identify the serovar of Escherichia coli O157:H7? What does the “O” and “H” stand for, respectively? Be sure to define any terms that you use in your response.Question. If you are working in a diagnostic lab, what will be your choice among Monoclonal or Polyclonal Antibodies method for detection of specific pathogen? Give sufficient justification for selection as well as rejection of any given methods.True or false? SDS PAGE gel can be prebed with NBT/BCIP to perform an immunoblot. The direction of proteins through a matrix is due to most proteins in nature having a net negative charge. PBST is buffer that is used for blocking the membrane during immunoloblotting.