Why are protein samples denatured before running SDS PAGE? A. To allow separation based on charged B. To be able to visualize the protein C. To allow separation based on size D. To be able to load samples correctly on the SDS-PAGE gel
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Why are protein samples denatured before running SDS PAGE?
A. To allow separation based on charged
B. To be able to visualize the protein
C. To allow separation based on size
D. To be able to load samples correctly on the SDS-PAGE gel
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- After running our protein samples on an SDS-PAGE gel, we will use a Coomassie-based dye (Gel Code Blue) to stain the gel. Based on your prior experience with Coomassie dye and understanding of its function, what do you predict this dye to be able to stain? A) Only protein tags B) Only DHFR protein C) Only recombinant proteins D) All proteinsIn primer designing, which of the following statements is correct? a. Primers should be 18-24 bases in length. b. Base composition should be 45-55% (G+C). c. Melting temperatures between 55-70°C are preferred. d. All choices are correct.What is the purpose of Bromophenol Blue in electrophoresis? a. To follow the progress of the gel as it is running b. It acts as a DNA size ladder or DNA size standards. c. To allow the electric current to move through the gel. d. To visualize the DNA after the gel has finished running. What is the purpose of acetate in TAE? a. It helps to maintain the pH of the buffer b. To carry electrons to allow the electric current to travel through the gel. c. It is used to stop the action of DNases d. It is required to see the DNA on a UV light.
- Give typing answer with explanation and conclusion The use of spin columns with silica for plasmid purification comprises an example of the following purification technique: a. adsorption to a matrix b. non-organic extraction c. organic extraction d. alkaline extraction e. none of the aboveIncorporating foreign DNA into the existing DNA of an organism will produce a different protein contrary to the one specified by the foreign DNA. Is it True or False?DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel? a. The 2000-bp fragment b. The 1000-bp fragment c. The 500-bp fragment d. All will migrate equal distances.
- This piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel.Which DNA fragment will be closest to the top (negative pole) of anelectrophoretic gel?a. 450 bp b. 3,560 bp c. 5 kb d. 1,500 bpThe chain termination method of sequencing: a. uses labeled ddNTPs b. uses only dideoxynucleotides c. uses only deoxynucleotides d. uses labeled dNTPs
- How could you improve upon your technique in order to get a better and more accurate standard curve graph? 2-Estimate (from your data) the [Protein] of your unknown based on comparing to the standards. Include the correct unit. This estimate should be an integer (no decimals).How are DNA fragments separated on an agarose gel duning electrophoresis ? A. The electric current makes the fragments migrate through the geThe smaller fragments migrate through the gel the fastest. B. The heavier fragments fall faster than the lighter ones and therefore, reach the end of the gel first C. The fragments are sorted based on the shape of their folding The more complicated the folded shape is, the slower the travel rate is. D. The larger fragments have more charge and, therefore, move through the gel faster than the smaller fragments John Doe has two possible fathers. The DNA fragment sizes of John Doe, John Doe's mother, a possible fathers are as follows: John Doe's mother - 850 bp and 400 bp John Doe - 680 bp and 400 bp Possible father 1 - 680 bp and 500 bp Possible father 11 - 680 bp and 280 bp Which, If either of the two men could be John Doe’s father and why? A. both of the two men, because they and John Doe all have a 680 bp sized DNA fragment B.…In analyzing the base composition of a DNA sample, a student loses the information on pyrimidine content. The purine content is A = 27% and G = 23%. Using Chargaffs rule, reconstruct the missing data and list the base composition of the DNA sample.
