Why are the last three dilutions plated, as opposed to only one dilution?

Appl Of Ms Excel In Analytical Chemistry
2nd Edition
ISBN:9781285686691
Author:Crouch
Publisher:Crouch
Chapter14: Chromatography
Section: Chapter Questions
Problem 9P
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The technician then plates 0.1mL of the last three dilutions in duplicate. Why are the last three dilutions plated, as opposed to only one dilution? Why are the dilutions plated in duplicate?

Dilution Scheme to Achieve 100 PFU/Plate
10 μιL
10 μι
66.66 µl
100 μL
100 μL
100 μι
1
1
1
1
1
1
100
100
15
10
10
10
Dilution #
1
3
4
6.
LB (Diluent) µl
990
990
933.34
900
900
900
Dilution Factor
102
104
1.5x105
1.5x106
1.5x107
1.5x108
PFU/0.1mL
1.5x107
1.5x105
104
103
102
10
1.5 x 1010
PFU/mL
Transcribed Image Text:Dilution Scheme to Achieve 100 PFU/Plate 10 μιL 10 μι 66.66 µl 100 μL 100 μL 100 μι 1 1 1 1 1 1 100 100 15 10 10 10 Dilution # 1 3 4 6. LB (Diluent) µl 990 990 933.34 900 900 900 Dilution Factor 102 104 1.5x105 1.5x106 1.5x107 1.5x108 PFU/0.1mL 1.5x107 1.5x105 104 103 102 10 1.5 x 1010 PFU/mL
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