The following is the PCR reaction that is programmed in the thermocycler to amplify the cheek cell DNA: 1.95°C for 2 minutes 2. 95°C for 30 seconds 3.58.1°C for 30 seconds 4. 72°C for 45 seconds 5. Repeat steps 2-4 thirty (30) times 6. 72°C for 3 minutes 7. Hold at 16°C indefinitely Which steps represent the denaturation, annealing and extension steps?
Q: When dispensing antihistamine drugs, what auxiliary label must be attached to the product? Explain i...
A: The auxiliary label has several names like, advisory label, cautionary label and warning lebel of dr...
Q: What is the intracellular voltage for Cl- if the intracellular concentration was 5 mM and the extrac...
A: The Nernst equation enables the calculation of the potential that will be established across the mem...
Q: Choose the monounsaturated fatty acid Select one : a. Linolenic acid b. Linoleic acid c. Oleic acid ...
A: Fatty acid is very important constituent in our body it is a long straight chain of carbon atoms wit...
Q: Which test can be used to differentiate ribose and glucose? Benedict’s Test Iodine Test ...
A: A molecule synthesized by living organisms or cells is referred to as a "biomolecule." Carbohydrates...
Q: CHOH CH,OH CH,OH OH CH OH но OH OH OH Answer: Answer CH,OH CH,OH CH,OH CH,OH он OH OH HO OH OH Answe...
A: Here all four options are of di-saccharides.
Q: Caffeine is an adenosine O antagonist O agonist
A: There are various bioactive compounds present in various plants. The bioactive compounds have an eff...
Q: A. Solubility Test Desi Ghee Vegetable Oil Refined Oil Water Ethanol Chloroform B. Translucent Spot ...
A: The lipids are soluble in organic solvents like chloroform and ether, insoluble in water. The qualit...
Q: 4. Molecule #4 a)What Group? (Carb, Lipid, Protein, or Nucleic Acid). b)Within the group, how would ...
A: The nucleotides are phosphorylated derivatives of nucleosides,. Nucleosides are compounds made up of...
Q: If sucrase is the enzyme that binds with sucrose, what is the name for the enzyme that binds with th...
A: Disaccharide is the type of carbohydrate which is made up of two monosaccharides. Sucrose is a disac...
Q: A. Solubility Test Desi Ghee Vegetable Oil Refined Oil Water Ethanol Chloroform B. Translucent Spot ...
A: a) Solubility test: Sample Desi ghee Vegetable oil Refined oil Water positive positive positive...
Q: Fetal gamma-subunits cause... Question 29 options: Decreased 2,3-bisphosphoglycerate binding a...
A: Red blood cells carry hemoglobin, which is a protein. Hemoglobin absorbs oxygen from the lungs and t...
Q: Imagine that you are a cell with 88 chromosomes undergoing meiosis How many chromosomes would your d...
A: The answer of the following question is given below
Q: Among the two methods of insect pest control - CULTURAL control or BIOLOGICAL control - which is the...
A: Pest control is simply defined as the management of a species defined as a pest. In pest mainly inc...
Q: 1d: Should PCR primers be complementary to each other? Explain your reasoning le: As you know, exper...
A: A primer dimer is a by-product in the polymerase chain reaction (PCR), consists of two primer molecu...
Q: 1a.Arrange these ligands in their order of potencies(high to low) 1b. Choose an appropriate concent...
A: Receptors are membrane proteins consisting of proteins, and glycans. Its extracellular domain contai...
Q: The following statements best describes the RNA structure EXCEPT
A: The answer of the following question is given below
Q: Explain why you agree or disagree with each of thefollowing statements:a. We should not worry much a...
A: Toxicity is defined as a substance that may be a chemical or any other component which creates a tox...
Q: . What is nanotechnology?
A: “Since you have asked multiple question, we will solve the first question for you. If you want any s...
Q: Identify the principles of the lyophilization process and list down the precautions and limitations ...
A: Lyophilization is the process of freeze-drying also called cryodesiccation. It involves ...
Q: Which of the following is the result of the hydrolysis of 5'-CTAGTTC-3' at the b side?
A: Nucleic acids are polymers of nucleotides (which consist of phosphate group, sugar and nucleobases)....
Q: reate an illustrated step-by-step schematic diagram of the following Qualitative test for Lipids: •...
A: Lipids are a heterogeneous class of naturally occurring biomolecules that are categorized based on t...
Q: When calibrating a pH meter, 3 (three) standard color-coded buffer solutions are used, which of thes...
A: pH is a quantitative measure of how acidic or basic is the aqueous or other liquid solution is. It t...
Q: A double helix makes one complete turn every 10 residues (3.4nm). Therefore the distance between bas...
A: The answer of the following question is given below
Q: Which of the following is the correct glycosidic bond linkage of the disaccharide shown? OH HO но Он...
A: Glycosidic linkage is formed between sugar molecules. The monosaccharide units are joined together t...
Q: Draw the Haworth structure of the tetrasaccharide:
A:
Q: Procedure: A. Peptic digestion 1. Prepare 4 test tubes as follows: Tube 1 - 3 ml pepsin Tube 2 - 3 ...
A: Digestion is the process by which complex macromolecules are broken down into simpler forms so that ...
Q: please complete the table (volume, dilution factor, concentration of glucose) and determine the conc...
A: The stock solution of glucose is prepared by dissolving 500mg of glucose in 25ml of distilled water....
Q: indly answer 13, 14, 15. Provide the letter of its answer 10. Which of the following types of membr...
A: Fabry disease: It is an inherited disorder that results from the accumulation of a particular type ...
Q: Explain in a more clinical detail the mode of actions of the following on the human skin cell or tis...
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any ...
Q: The BSA stock solution from the previous problem was then diluted to generate a set of standard solu...
A: CONCENTRATION OF BSA (mg/ml) ABSORBANCE 0 0 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8 ...
Q: The Sanger method of DNA sequencing follows the principle of complementarity just like in the replic...
A: Sangers method also known as chain termination method is a method of DNA sequencing.
Q: o-H H. Hy-H H. From the N-terminus, this sequence is found on the 125th residue onwards. Based on th...
A: Proteins have different structures at its level of complexity. Simple chain of amino acids forms pri...
Q: In dialysis, large molecules are allowed to diffuse through a semi-permeable membrane. True Fals...
A: Dialysis : Separates molecules in solution via the difference in the diffusion rates through the sem...
Q: Conc. H,SO, a-naphthol (*) Conc. HSO, acetic anhydrde Orcinol, FeCi, HCI A () Cu(CH,COO), acetic aci...
A: The schematic diagram that is represented is showing the results of different biochemical tests for ...
Q: Three buffers are made by combining a 1M solution of acetic acid and a 1M solution of sodium acetate...
A: Given Values: Ka of acetic acid = 1.7×10-5 The concentration of acetic acid stock solution = 1 M The...
Q: Full meaning of ATP
A: There are different biomolecules present in a cell that serve important roles in cellular functionin...
Q: Enzymes normally enhance the rates of biochemical reactions by preferentially binding and stabilizin...
A: ATP synthase is the enzyme complex that undergoes multiple subunits and thus plays an integral role ...
Q: TRUE OR FALSE a) The structure of the purines and pyrimidines make them able to undergo keto-enol t...
A: The structure of the purines and pyrimidines make them able to undergo keto-enol tautomerism.
Q: 10.18 Using the information in Figs. 10.14 and 10.18, explain how the two oligonucleotides 5'-CAAAGA...
A: Deoxyribonucleotides are the building blocks of DNA. Each nucleotide is composed of five carbon suga...
Q: efer to the figure below. Replacing lysine with another amino acid in the protein may alter the shap...
A: Proteins: Proteins are an important class of biological macromolecules that are polymers of amino a...
Q: Characterization of enzyme activity does not allow us to: a. determine how different variables affec...
A: Enzymes are biocatalyst that increases the speed of reaction by lowering the activatio...
Q: Which are the mono- and dsaccharide hydrolysis products of starch with α-amylase? glucose and ...
A: Alpha amylase hydrolyses alpha linked polysaccharides like starch and glycogen at alpha bond positio...
Q: Assay of Serum Alkaline Phosphatase. Serum component of blood is preferred to be use than plasma, wh...
A: Alkaline phosphatases are located on the outer layer of the cell membrane and catalyzes the hydrolys...
Q: Which of the following protein(s) exhibit positive cooperativity in oxygen binding? a) Myoglobin b...
A: Both myoglobin and hemoglobin are proteins with a quaternary structure. Myoglobin is present in the ...
Q: Calculate the standard free energy change for each of the following metabolically important enzyme-c...
A: All living organisms are made up of cells, which are the most basic unit of life. They are responsib...
Q: True or False. Write the word “reducing” if the statement is correct, otherwise write “non-reducing”...
A: Mutarotation is the change in the optical rotation as change in the equillibrium between two anomers...
Q: Question 5: (a) Why is it important that gluconeogenesis is not the exact reversal of glycolysis? (b...
A: Glycolysis is a metabolic pathway in which glucose is converted into two molecules of pyruvate in th...
Q: What gives the DNA molecule a negative charge? nucleobases deoxyribose ribose phosphate ...
A: All living organisms are made up of cells, which are the most basic unit of life. They are responsib...
Q: Suggest whether the following structures are likely to act as cholinergic agonists or not and briefl...
A: Cholinergic agent: The compound which mimic the action of acetylcholine or butyrylcholine are called...
Q: All of the following are parts of the honeybee's processing of honey EXCEPT: producing a small...
A: The honeybee is a fantastic flying mechanism. She can carry a nectar or pollen payload that is close...
Answer the correc option
not need explanation
need ASAP
Step by step
Solved in 3 steps
- Choose the one answer that fits best. In PCR, which of the following is NOT a major component of the reaction mix (videos)? a. Nucleotides b. Buffer c. Template DNA d. Thermophilus aquaticus e. Primers1. You are working as a CSI analyst. You collected DNA at a crime scene that is from the suspect but do not have enough to run a test to determine who the suspect is. Please discuss what you would do to attain more of the DNA and how to test the DNA to determine who the suspect is.2. Please discuss how the bacterium Agrobacterium tumefaciens normally functions. Please also discuss how this bacterium can be used to help plants attain genes to help fight off insects and herbicides.The laboratory has lost the protocol to follow during the PCR reaction and only give you this. Please fill in the blanks: 94°C _______________ step for _______ minutes 52°C _______________ step for _______ seconds 72°C________________ step for ______ minutes 72°C _______________ step for ______ minutes
- Imagine that you correctly prepare your PCR reaction mixture, but there is something wrong with the thermal cycler. Describe what would happen if: The thermal cycler was stuck on 940C: The thermal cycler cycled between 600C and 720C, but never reached 940C The thermal cycler cycled between 940C and 720C, but never reached 600Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.During real-time PCR, the synthesis of PCR products is analyzeda. at the very end of the reaction by gel electrophoresis.b. at the very end of the reaction by fluorescence that is emittedwithin the thermocycler.c. during the PCR cycles by gel electrophoresis.d. during the PCR cycles by fluorescence that is emitted withinthe thermocycler.
- Can you say that the microbial composition changes over time in each of the body locations? Give two pieces of evidence. Why do you think the scientists also tested the soil around the cadaver? The study was done with mice and their cadavers decomposed on soil graves in the University of Colorado Transgenic Facility. Do you think the graphs would have looked different if the decomposition events would have happened outside during the hot summer months? Explain.When using a micro centrifuge, what should you check before starting the machine? A. That the samples are balanced B. That the PCR tube caps on the samples are slightly open C. That the machines is set to 64 degrees Celsius D. That is programed for 35 cycles.In a campus-wide screen for microbes, you find DNA from the bacterial species that causes the bubonic plague, Yersinia pestis. You take the sample back to the lab, and try to grow (very carefully), but can't culture the organism. Provide two possible explanations for why you can't grow this sample in the lab.
- Why is the DNA polymerase used in PCR derived from an extreme thermophile bacteria species rather than a mesophile bacteria species? (be sure to describe the mesophile DNA polymerase as well as the extreme thermophile DNA polymerase.)discuss the method ATP-TOX as an old method and PCR as a new method for water biomentoring method and where does it fit in with existing testing and why is it relevent and new?How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be to the point.