Why is it important to maintain a constant volume of water in your water bath (when boiling nucleic acid fractions) for chromatography.

Principles Of Radiographic Imaging: An Art And A Science
6th Edition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Chapter5: The X-ray Tube
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Why is it important to maintain a constant volume of water in your water bath (when boiling nucleic acid fractions) for chromatography. 

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