Given the chromatogram below in a normal phase, which samples would be the most polar? A O Samples C and D O Samples Band X O Samples B and C O Samples A and D
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- A molecule into which a radioisotope has been incorporated can be used as an ____________. a. compound c. salt b. tracer d. acidWhen a mixture of various molecular sized dyes are run through a gel chromatography column, what is eluted in the Void Volume? A. Blue Dextran B. Flourescein C. Both D. NothingIn which type of chromatography lipids are carried up a silica gel coated pate by a rising solvent front, less polar travels farther than the more polar ones?a) Absorption chromatographyb) Thin layer chromatographyc) Gas-liquid chromatographyd) HPLC
- Justify and explain following statements. (a) Paper chromatography is a type of partition chromatography. (b) The R f value is 1. (c) Chromatography is only a qualtitative technique. (d) The adsorbent should be insoluble in the solvent. (e) Chromatography is used to separate only color compounds.A sample was placed on a chromatography column. Dichloromethane was used as the eluting solvent. All the components were eluted off the column, but no separation was observed. What must have been happening during this experiment? How would you change the experiment to overcome this problem?A student performs a separation by TLC on a silica get plate that results in three spots. Ared spot is measured 23 mm away from the original sample spot, a blue spot is 42 mmaway, and a yellow spot is 49 mm away. The distance from the original spot to where thesolvent stopped is 55 mm.a) Calculate the Rf value for each component in the mixture. (Show your work and reportthe correct number of significant figures.) b) The silica gel plate is polar. Rank the three components in the mixture from lowest tohighest polarity. Explain your answer.
- In which type of chromatography, solvents of increasing polarity are passed through a column of silica gel?a) High performance liquid chromatographyb) Thin layer chromatographyc) Adsorption chromatographyd) Gas-liquid chromatographyshow the complete solution and write answers in 3 significant figures. M&M's color COMPOSITION Orange ? Green ? Blue ? Brown ? Chromatogram before and after:1. What would be the effect of the following errors in chromatographic work? a. The solvent level in the developing chamber is higher than the spotted sample. b. Too much sample is applied to the paper. c. The paper is allowed to remain in the chamber after the solvent front has reached the top of the plate.
- Which of the following statements is TRUE about the band of molecules labeled X ( close to where it says positive electrode). A) it contains DNA fragments that are shorter than the ones in any of the other bands. B) it contains fewer DNA molecules than any of the other bands from lane 3. C) it contains more negatively charged particles than any other band on the gel. D) it contains fewer genes than any of the other bands on the gel.A student carries out the following experiments and records the following observations. 1. A few drops of a solution of KOH (aq) is added to a solution of Fe(NO3)3 (aq). A red gelatinous solid forms immediately upon addition. 2. A few drops of a solution of KOH (aq) is added to a solution of NaNO3 (aq). No changes are observed. 3. A few drops of a solution of KOH (aq) is added to a solution that could contain Fe3+(aq) and/or Na+(aq) ions (the solution could contain both ions, just one ion, or neither ions). A red gelatinous solid forms immediately upon addition. Select all of the answers that are consistent with the data from those 3 experiments. Group of answer choices The unknown contains Fe3+(aq) and Na+(aq) The unknown contains Fe3+(aq) It is not possible to determine the presence of Na+(aq) from this experiment The unknown contains neither Fe3+(aq) or Na+(aq)If you want your molecule’s fluorescence to not be quenched then you shoulda. decrease temperatureb. increase temperaturec. add O2
