Given the chromatogram below in a normal phase, which samples would be the least polar? O Samples B and C O Samples C and D O Samples A and D O Samples B and X
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- Polonium is a rare element with 33 radioisotopes. The most common one, P210o, has 84 protons and 126 neutrons. When P210o decays, it emits an alpha particle, which is a helium nucleus 2 protons and 2 neutrons. P210o decay is tricky to detect because alpha particles do not carry very much energy compared to other forms of radiation. For example, they can be stopped by a single sheet of paper or a few inches of air. That is one reason why authorities failed to discover toxic amounts of P210o in the body of former KGB agent Alexander Litvinenko until after he died suddenly and mysteriously in 2006. What element does an atom of P210o change into after it emits an alpha particle.When a mixture of various molecular sized dyes are run through a gel chromatography column, what is eluted in the Void Volume? A. Blue Dextran B. Flourescein C. Both D. NothingA sample was placed on a chromatography column. Dichloromethane was used as the eluting solvent. All the components were eluted off the column, but no separation was observed. What must have been happening during this experiment? How would you change the experiment to overcome this problem?
- Justify and explain following statements. (a) Paper chromatography is a type of partition chromatography. (b) The R f value is 1. (c) Chromatography is only a qualtitative technique. (d) The adsorbent should be insoluble in the solvent. (e) Chromatography is used to separate only color compounds.In which type of chromatography lipids are carried up a silica gel coated pate by a rising solvent front, less polar travels farther than the more polar ones?a) Absorption chromatographyb) Thin layer chromatographyc) Gas-liquid chromatographyd) HPLCThe reason why a nucleus might emit radiation is because by doing so, it forms a more stable-lower energy nucleus.True false
- show the complete solution and write answers in 3 significant figures. M&M's color COMPOSITION Orange ? Green ? Blue ? Brown ? Chromatogram before and after:A student performs a separation by TLC on a silica get plate that results in three spots. Ared spot is measured 23 mm away from the original sample spot, a blue spot is 42 mmaway, and a yellow spot is 49 mm away. The distance from the original spot to where thesolvent stopped is 55 mm.a) Calculate the Rf value for each component in the mixture. (Show your work and reportthe correct number of significant figures.) b) The silica gel plate is polar. Rank the three components in the mixture from lowest tohighest polarity. Explain your answer.If you have a sample of unknown composition, a first step at analysis might be a determination of the masses of the atoms and molecules in the sample. A mass spectrometer to make such an analysis can take various forms, but for many years the best technique was to determine the masses of ionized atoms and molecules in a sample by observing their circular paths in a uniform magnetic field, as illustrated is shown. A sample to be analyzed is vaporized, then singly ionized. The ions are accelerated through an electric field, and ions of a known speed selected. These ions travel into a region of uniform magnetic field, where they follow circular paths. An exit slit allows ions that have followed a particular path to be counted by a detector, producing a record of the masses of the particles in the sample. The moving ions can be thought of as a current loop, and it will produce its own magnetic field. The direction of this field at the center of the particles’ circular orbit isA. In the…
- Why is it important to maintain a constant volume of water in your water bath (when boiling nucleic acid fractions) for chromatography.Would you expect a change in column temperature to have an effect on a reversed-phase chromatographic separation of a steroid mixture? Explain the reason for your answer in (1) abovewhen solar radiation crosses space and moves towards earth.it consists of many types of radiation together these radiation types are known as electromagnetic spectrum. which properties differentiates among the different types of radiation found in the electromagnetic spectrum.