What does whole exome sequencing test for?

Q: What is linker scanning mutagenesis?

A: Answer: Introduction: Old-style approaches, containing linker-scanning mutagenesis includes…

Q: What is metagenome sequencing?

A: Metagenomics is the study of microbial communities in their environment by using modern genomic…

Q: Which of the following cloning vectors have the minimal features required for cloning ? Answer =

A: Answer :: Brief description about cloning Cloning is a process that allows scientists to create…

Q: What is the ENCODE project?

A: ENCODE is Encyclopedia of DNA Elements. It is a project funded by National Human Genome Research…

Q: Why is genome database and annotation relevant to Genome sequencing?   Are there any examples?

A: Genome: the set of genes. Genome database: it is the collection of all information on the human…

Q: What is positional cloning?

A: Gene cloning is the process in which any gene of interest is amplified, that is, several copies of…

Q: Why is Sanger sequencing sometimes referred to as "dye-terminator" sequencing?

A: Frederick Sanger and his colleagues created Sanger Sequencing, often known as 'chain termination…

Q: What vectors can be used for cloning?

A: Introduction: Vectors are the molecular vehicles used for the transfer of genes of interest into the…

Q: How are genes recognized within genome sequences?

A: Meaning of Genes: Genes refers to a specific sequence of nucleotides in DNA or RNA that is located…

Q: What are selectable markers and why are they important to cloning vectors?

A: In recombinant DNA technology the vectors are the carrier for the target DNA sequence which needed…

Q: What are gene microarrays? How are microarrays used?

A: The process of forming a single double-stranded nucleic acid molecule by combining two…

Q: What provides a convenient bridge between the low resolution of a karyotype and the ultra-high…

A: Introduction  Cytology refers to the study of cell such as cell morphology, physiology etc. As we…

Q: How to identify and analyze all functional elements of the genome ?

A: Introduction: The complete set of hereditary data present in a living being is referred to as the…

Q: How is recombinant DNA inserted into a genome using CRISPRediting?

A: The modern technology of genetics can be utilized to increase the genomes of various living…

Q: What is a cloning vector? Give two examples.

A: A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into…

Q: What is the difference between using genome build hg18 and hg19 in Genome-wide SNP arrays?

A: SNP (Single nucleotide protein) array is a type of DNA (deoxyribonucleic acid) microarray which is…

Q: How is exome sequencing helps in identifying mutations?

A: Exome sequencing refers to the genomic technique in which the sequencing of all the protein-coding…

Q: Why are the proteins cleaved into small fragments for protein sequencing?

A: Proteins are the end product of the molecular translation that codes the information present in the…

Q: Define the Single-cell sequencing (SCS) ?

A: Working of Single-cell deoxyribonucleic acid genome sequencing involves analytic one cell,…

Q: How to use a microarray for transcriptome analysis ?

A: Transcriptome is the complete set of the mRNA molecules expressed in an individual . It is termed as…

Q: What does whole exome sequencing reveal?

A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…

Q: Define about Sanger sequencing ?

A: Introduction:- The process of determining the sequence of nucleotide bases (As, Ts, Cs, and Gs) in a…

Q: What are the steps in shotgun sequencing?

A: Shotgun sequencing is a laboratory method which is used to determine the sequence of DNA of an…

Q: Give a step by step method and diagram of how reversible terminator sequencing works.

A: The reversible terminator sequencing or Illumina dye sequencing is a technique used to determine the…

Q: How to find unknown gene and design primers present using whole genome sequence?

A: Step 1 Whole genome sequencing is the process that regulates the complete deoxyribonucleic acid…

Q: What is the main or primary use of positional cloning?

A: Positional cloning refers to the method used to identify a trait-associated gene based on its…

Q: What does whole genome sequencing reveal?

A:

Q: What is shotgun cloning or shotgun sequencing ?

A: Cloning is the process of creating an identical copy of something. In biology, it refers to the…

Q: How many male/female sheep are needed to clone one? Cancloning be done by using adult cells?

A: One male and one female sheep is required to clone one because we need one male gamete and one…

Q: When is low diversity especially problematic in sequencing?

A: In sequencing micro RNA any method which requires use of in house barcodes may results in giving low…

Q: What is gene sequencing used for?

A: DNA sequencing is the process of determining the sequence of nucleotide bases that make up a DNA.…

Q: What is a single-nucleotide polymorphism (SNP)? How are SNPs used in genomic studies?

A: Single nucleotide polymorphisms ( SNPs) are a type of polymorphism that involves a single base pair…

Q: How much of the human genome is coding?

A: The genes are the components of the genome that direct specific functions in the cell. Their…

Q: Why are next generation sequencing reads determined after negative selection, while induction values…

A: Introduction :- The process of determining the primary structure of an unbranched biopolymer through…

Q: What is the difference between a map-based approach to sequencing a whole genome and a whole-genome…

A: Deoxyribonucleic acid (DNA) is a double stranded helical genetic material containing thousands of…

Q: How does positional cloning work and What is the basic principle of positional cloning?

A: Positional cloning is a technique used in laboratories for locating the position of…

Q: What are cloning vectors ?

A: Cloning is the process of making identical copies of a DNA sequence.

Q: What is a Sanger Sequencing ?

A: The term DNA sequencing encompasses biochemical methods for determining the order of the nucleotide…

Q: Explain how Sanger sequencing works

A: Genome sequencing: genome means total gene content present in the organism and sequence means to…

Q: What is the primary disadvantage of Sanger sequencing?

A: Sanger sequencing the target DNA sequence is determined by copying it into the fragments of…

Q: What are Short hybridization probes?

A: A probe is a stretch of single stranded ribonucleotides (RNA) or deoxyribonucleotides (DNA). DNA…

Q: What can exome sequencing do for you?

A: The sequencing of the nucleic acid is the process through which the exact sequence of the nucleotide…

Q: How would one recognize a gap in the genome sequence following nucleotide sequencing?

A: DNA is made up of four bases namely adenine (A), thymine (T), cytosine (C), and guanine (G). The…

Q: What is the largest genome?

A: Genome is the genetic material of an organism. It consists of DNA. Genome is of two types- Nuclear…

Q: What is an STS? How are STSs generated experimentally? What are the uses of STSs? Explain how a…

A: BASIC INFORMATION GENE MAPPING  This is a technique to identify the position of elements in the…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.