Why is Taq polymerase used in PCR rather than other DNA polymerases?a. Taq polymerase is a synthetic enzyme that produces DNA strands ata faster rate than natural polymerases.b. Taq polymerase is a heat-stable form of DNA polymerase that canfunction after exposure to the high temperatures necessary for PCR.c. Taq polymerase is easier to isolate than other DNA polymerases.d. Taq polymerase is the DNA polymerase commonly produced bymost eukaryotic cells.e. All of the above are correct.

Why is Taq polymerase used in PCR rather than other DNA polymerases?a. Taq polymerase is a synthetic enzyme that produces DNA strands ata faster rate than natural polymerases.b. Taq polymerase is a heat-stable form of DNA polymerase that canfunction after exposure to the high temperatures necessary for PCR.c. Taq polymerase is easier to isolate than other DNA polymerases.d. Taq polymerase is the DNA polymerase commonly produced bymost eukaryotic cells.e. All of the above are correct.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 12QP

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Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.
(A) After three cycles of PCR, how many DNA molecules are present that correspond precisely to the desired amplification product? (B) What about after 5 cycles. Assume that we started with one molecule in each case, and that the reaction is perfectly efficient.
Would it be possible to use human polymerase for the PCR reaction? a. No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur. b. No, because human polymerase cannot be extracted from cells to use in a lab setting. c. Yes, because we are using human DNA as the template DNA. d. Yes, because human polymerase can add bases to a template strand without a primer.
What was the purpose of the sodium chloride in nucleic acid extraction? CHOOSE ALL ANSWERS THAT APPLY A Sodium chloride binds mostly to the proteins associated with the DNA. B Sodium ions bind to the phosphate groups in DNA. C Chloride ions bind to the phosphate groups in DNA. D Sodium chloride shields the DNA protecting it from degrading, E Sodium ions bind to the deoxyribose in the DNA.
Choose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)? a. PCR requires a copy of RNA that serves as a template b. Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA c. Annealing requires cooler temperatures than denaturation d. Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA e. PCR is a quick way of using minute quantities of DNA to create millions of copies
In the dideoxy-sequencing reaction, what terminates DNA synthesis at a particular base? a. The absence of a base on the ddNTP halts the DNA polymerase. b. The ddNTP causes a break in the sugar–phosphate backbone. c. DNA polymerase will not incorporate a ddNTP into the growing DNA strand. d. The absence of a 3′-OH group on the ddNTP prevents the addition of another nucleotide.
Polymerase Chain Reaction, or PCR, can Group of answer choices A. target a specific region of DNA and cut it out of the rest of the genetic material for further analysis. B. copy the number of copies of a selected region of DNA linearly. C. increase the number of copies of a selected region of DNA exponentially. D. copy the entire genome at least a dozen times.
a. Explain why the PCR is unlikely to amplify contaminatingbacterial DNA in a sample of human DNA. b. Explain how PCR could be used to pick a gene out of a complexgenome and amplify it.
DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel? a. The 2000-bp fragment b. The 1000-bp fragment c. The 500-bp fragment d. All will migrate equal distances.
a. Explain whether or not DNA polymerase from a mesophilic bacterium could be used successfully in a PCR reaction. b. If starting with a single double-stranded DNA molecule, how many copies of the DNA would be synthesized after 25 PCR cycles?
A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Which of the following results is he most likely to observe? a. The reaction will work, but at a significantly slower rate. b. The reaction will work, but the product will contain many undesired mutations. c. The reaction will work, but amplify a region that was not his target. d. The reaction will be completely unsuccessfu
a. What determines the size (length) of the primary PCR product? b. What might a successful gel check of a PCR reaction look like?