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Why would you want to limit the number of times you passage a cell culture line?
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- You are incubating cells for 3 hours. The initial number of cells present in the sample were 102. After the incubation period, you find that the culture now has 104 cells. What is the generation time in minutes?You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours. a) How many generations occurred?b) How many cells will be present after the 10 hours?What are the differences between “primary cell culture” and “continuous cell lines”?.
- What is secondary cell culture?How would I figure out how many dilutions to make given cell density ? And volume to plate ? For example, I want to plate 0.1 ml and want 200 colonies how many dilutions would i need to make, given 7.5 x10^5 cells/ mlYou are growing a culture of S. aureus. You know that 9 generations have occurred after 6 hours. What is the generation time in minutes? if i started a culture with 1000 cells, after 8 generations, how many cells will i have?
- Features of continuous cell culture include the following, except ________ . a.addition of product b. stirring c.addition of nutrients d.removal of product e.sterilization of nutrientsHow long would it take a single bacterial cell to form 1,000,000 cells if it had a generation time of 30 minutes?A culture with approximately 3x104 cells/mL were incubated. After 3 hours, the number of cells had increased to 6x105 a) How many generations have occurred?
- Your first task in the lab is to learn how to culture these cells and begin expanding the culture (increasing the # of cells) so that you may later conduct experiments on them. You take two aliquots, each with an equal number of cells, and place them in two different culture vessels, one is a sealed airtight flask (culture 1) and the other is an open topped culture dish (culture 2). You provide both with the same growth media that contains their preferred fuel source, glucose. Will one of these cultures (#1 or #2 as described above) need to have their media changed more frequently than the other? (Yes or No) If yes, which culture will need to have its media changed more often and why? If no, why not?A type of cell culture that can reproduce for an extendednumber of generations and is used to support viral replicationis a:(a) Primary cell culture (d) Diploid fibroblast cell(b) Continuous cell line (e) Connective tissue(c) Cell strainWhy must primary cell cultures be restarted every so often when preparing primary cell cultures to observe morphological changes caused by cells infected by a virus? Why are tumor cells preferred?