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- flow chart of purification steps of Beta-fructofruanosidase (Invertase)Why is COVID-19 a man made? (Brief explanation only atleast 3-5 sentences)Rabbits were in injected with [32 P] photsphate intraperitoneally ans asacrificed after 2, 5, 8, 12, 18, 24, 48, or 168 hours of radioisotope administration. DNA, nuclear RNA (nRNA), and cytoplasmic RNA (cRNA) were prepared from the kidneys and their specific activities (radioactivity/ mg of nucleic acid) were determined 1. what process was studied in this expereiement 2. What is concluded about the division of kideny cells during the time of labeling and why.
- Why is RNA yeast soluble in diluted NaOH solution?Explain why protein purification procedures are normally carried out 4-5°C.One of the members of your research group attempted to isolate theenzyme using the protocol you presented. To confirm whether theygot the protein, SDS-PAGE was performed and the gel on the rightwas obtained. Perform the necessary calculations to determine themolecular weight of the suspected band on the gel in the lanelabeled “dialyzed.”
- The SARS-Cov-2 virus is the virus that cause COVID-19. The viral protease is a known target for finding potential viral inhibitor. The activity of the viral protease can be assayed using the synthetic peptide substrate attached to 7-amino-4-carbamoylmethylcoumarin (PACC). The viral protease cleaved the substrate producing the fluorogenic ACC which can be monitored using a spectrofluorometer. The following is data from SARS-Cov-2 protease (0.1 mg of enzyme) upon reaction with various concentrations of the substrate PACC. Plot the appropriate graphs to find: - The approximate Vmax from the Michaelis-Menten curve.Salting out and gelation of proteins is most advisable to carry out when biopolymers are in the isoelecric state. Specify the pH which corresponds to this stateThe purification of cytochrome C begins with 1) yeast homogenization using a bead beater in the presence of BME (a reducing agent) and a protease inhibitor from approximately 900 grams of Baker’s yeast. Then, 2) insoluble cell contents were removed by centrifugation at 4,000 x g for approximately ten minutes. The ruptured cells (lysate) after centrifugation had a total volume of 0 mL and a 1.0 mL aliquot was set aside for further analysis. The following data was obtained from the 1.0 mL aliquot to quantify the protein amount and purity: The absorbance at 410 nm of the aliquot was 0.460 (1 cm pathlength). The absorbance at 595 nm from a 1.0 mL Bradford Assay solution that was diluted by 250-fold from the aliquot was 0.681 (1 cm pathlength). Using the information given, Calculate the total protein amount in mg from the absorbance at 595 nm. Calculate the cytochrome C amount in mg from the absorbance at 410 nm using Beer’s Law.