You analyse some DNA using an automated sequence reaction. The first peak you observe is labeled with a red fluorescent label. From this you can conclude that the most base in the DNA is a containing the red fluorescent label. Select one: 5'; dideoxynucleotide. a. O b. 3'; deoxynucleotide. O c. 5'; deoxynucleotide. d. 3'; dideoxynucleotide. None of the other answers given are correct. е.

You analyse some DNA using an automated sequence reaction. The first peak you observe is labeled with a red fluorescent label. From this you can conclude that the most base in the DNA is a containing the red fluorescent label. Select one: 5'; dideoxynucleotide. a. O b. 3'; deoxynucleotide. O c. 5'; deoxynucleotide. d. 3'; dideoxynucleotide. None of the other answers given are correct. е.

BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)

10th Edition

ISBN:9781305967359

Author:STARR

Publisher:STARR

Chapter15: Biotechnology

Section: Chapter Questions

Problem 6SA

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If DNA of a particular species was analyzed and it was found that it contains 27 percent A, what would be the percentage of C? a. 27 percent b. 30 percent c. 23 percent d. 54 percent
Your lab partner tells you proteins are used to make enzymes, you say, no enzymes are used to make proteins. Who is right? Explain your answer. – realize that enzymes are proteins and that the enzyme polymerase is used to transcribe DNA
In the dideoxy-sequencing reaction, what terminates DNA synthesis at a particular base? a. The absence of a base on the ddNTP halts the DNA polymerase. b. The ddNTP causes a break in the sugar–phosphate backbone. c. DNA polymerase will not incorporate a ddNTP into the growing DNA strand. d. The absence of a 3′-OH group on the ddNTP prevents the addition of another nucleotide.
What is DNA polymerase? a.An enzyme that carries out DNA replication b.Short, single strand of DNA that base-pairs with a specific DNA sequence c.An enzyme that corrects mutations that arise during the replication of DNA d.An enzyme that seals any gaps that remain between bases of replicating strands of DNA
What was the purpose of the sodium chloride in nucleic acid extraction? CHOOSE ALL ANSWERS THAT APPLY A Sodium chloride binds mostly to the proteins associated with the DNA. B Sodium ions bind to the phosphate groups in DNA. C Chloride ions bind to the phosphate groups in DNA. D Sodium chloride shields the DNA protecting it from degrading, E Sodium ions bind to the deoxyribose in the DNA.
Suppose a region of DNA is 100 bp long. How many unique sequences could it potentially represent? A) 1004 B) 4100 C) 400 D) 4,000 E) 10,000
The figure below shows a polynucleotide:a) Label the 5' and the 3' end of the sequenceb) If a nucleotide were added to this polynucleotide, which end would it be added to?c) Is this an RNA or DNA strand? How do you know?
Place the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.
What is DNA polymerase? A. An enzyme that corrects mutations that arise during the replication of DNA B. An enzyme that seals any gaps that remain between bases of replicating strands of DNA C. Short, single strand of DNA that base-pairs with a specific DNA sequence D. An enzyme that carries out DNA replication
Chargaff studied the composition of DNA from different sources and found thata. the number of phosphate groups always equals the number of five-carbon sugars.b. the proportions of A equal that of C and G equals T.c. the proportions of A equal that of T and G equals C.d. the proportions of deoxyribose equal that of ribose.
GENETICS The modified "dye terminator method for DNA sequencing represented a major improvement over Sanger's original method because ( among other things) it does NOT require a) a DNA primer b) DNA polymerase c) the use of four separate sequencing reactions for each template d) the use of electrophoresis to separate DNA chains based on size e) the used chemically modified dNTPs f) all of the above
Polymerase Chain Reaction, or PCR, can Group of answer choices A. target a specific region of DNA and cut it out of the rest of the genetic material for further analysis. B. copy the number of copies of a selected region of DNA linearly. C. increase the number of copies of a selected region of DNA exponentially. D. copy the entire genome at least a dozen times.