You are developing a method for the analysis of the compounds listed below. You have a C18 column, water, methanol, and any required additives available to develop this method. You sample contains 2-methyl-pentan-1-oic acid, pentanoic acid, pentanol, and pentylamine. • Is plain water sufficient to provide reproducible methods? Why or why not? o If you have to add something to your mobile phase, what would you add and why?
Chemistry
Question 2
You are developing a method for the analysis of the compounds listed below. You have a C18 column, water, methanol, and any required additives available to develop this method. You sample contains 2-methyl-pentan-1-oic acid, pentanoic acid, pentanol, and pentylamine. • Is plain water sufficient to provide reproducible methods? Why or why not? o If you have to add something to your mobile phase, what would you add and why? • What do you anticipate will be the elution order of these compounds? Why? • If I switched to a silica column with hexane as my mobile phase (assume solubility), what would be the new elution order? Why? The answers to these questions can be found in the Introduction and Mobile Phase slide
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