You are given an unknown bacterium in the lab and have to determine unknown bacterium's oxygen needs. You place the organism in a shake tube and after 24-hours observe uniform turbidity throughout the media. How do you classify this organism? O Anaerobe O Facultative anaerobe O Microaerophile O Aerotolerant
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- A prokaryotic cell hitched a rideto planet earth on a space shuttle from some unknown planet. The oiganism is a psychrophile, an obligate halophile and an abligate aerobe. a) Based on the charateristics of this microbe, describe the planet (3 descriptors) b) Could this organism survive on your kitchen counter? and why?a. What oxygen requirements do your think this bacterium will have (obligate aerobe, obligate anaerobe, facultative anaerobe, aerotolerant anaerobe, or microaerophile), and why? b. You run a thioglycollate test of your isolated bacterium. Based on your response to Part A, which thioglycollate tube best represents the result you would expect for your bacterium? Explain your response. If the result you would expect is not represented, explain what the tube should look like. c. What would you expect to be the result of a catalase test of your bacterium? d. What techniques or specialized equipment, if any, would you need to grow cultures of this bacterium?Escherichia coli O157:H7 is a foodborne pathogen and can cause serious illness in humans by producing toxins that can severely damage the lining of intestines and kidneys. What are the oxygen requirements of coli? What result would you observe in the MTM agar deep test? Explain. Research on the growth requirements of this bacterium is required. What is the role of oxygen and cytochrome c oxidase in aerobic respiration? Why does the nitrate reduction tube turn red after the addition of zinc? Clostridium tetani is a common soil bacterium and causes tetanus. Would you expect tetani to possess the enzyme catalase? Explain. Research on the growth requirements of this bacterium is required.
- Out of the 5 possible oxygen requirements (Obligate aerobes, microaerophiles- is E. coli, Pseudomonas aeruginosa, and Clostridium sporogenes? sketch below (or describe well with words), what growth would look like for each species in FTM tubes.Helicobacter pylori, a bacterium that causes stomach ulcers, can grow in medium with a pH above 5. It can survive but not grow in pH 2 medium as long as the medium contains Urea. You inoculate a pH 2 broth with 1,000,000 H. pylori and for 8 hours the organism did not grow. The next morning however, the medium has become quite turbid, suggesting that the organism has grown. Explain how this could happen. What phylum and class does this organism belong?Suppose you would like to determine the number of bacteria in a culture via anaerobic plate count. Explain (with pictures)how you would set up a dilutionseries (10-4,10-6, 10-8) usingthe fewest number of dilution blanks.Draw andlabel your tubes and plates with each dilution. Show all volumes including transfers, amounts in tubes, and amounts plated.
- A certain medium has the following composition:Glucose 15 gYeast extract 5 gPeptone 5 gKH2PO4 2 gDistilled water 1,000 mla. Tell what chemical category this medium belongs to, and explainwhy this is true.b. How could you convert Staphylococcus medium intoa nonsynthetic medium?If you altered the conditions under which bacterial growth normally occurs (i.e. increase the temperature of incubating a culture from 35 to 40°C), what effect would this have on the bacterial growth curve of the organism under study?1. Clostridium butyricum does not appear to have superoxide dismutase (SOD) and catalase enzymes. How would the growth of C. butyricum in oxic conditions compare to its growth in anoxic conditions? On this basis, what is the aerotolerance category of C. butyricum? 2. Citrobacter freundii is a facultatively anaerobic bacterium. Why would you expect this bacterium to grow better (i.e. form larger colonies) in an oxic environment than in an anoxic one? (as a hint, do some research on the “Pasteur effect”)
- Suppose you spilled two cultures of Salmonella typhimur-ium (each containing 100,000 cells) on your lab bench. You im-mediately applied the same disinfectant to both cultures at thesame time. One culture had been freshly grown for 36 hours andthe other culture is 2 weeks old. If the kill ing rate of the disin-fectant is 90% per minute, do you think that microbes in bothcultures will be completely killed after 6 minutes? Why or whynot?A chemolithoautotroph is respiring using only iron (Fe3+) and nitrite (NO2-). This bacterium does not consume organic molecules. From where does this organism obtain its carbon?Hi can I get help on this microbiology question please? I am stuck and do not understand it. Thank you A prokaryotic cell hitched a ride to Earth on a space shuttle from some unknown planet. Microbiologists studied the microorganism and concluded it is a psychrophile, an obligate halophile, and an obligate aerobe. 1. Based on the characteristics of the microorganism described in this case, describe at least three characteristics that the unknown planet where the microorganism lived before must have so that it can survive in it. 2. Explain the following microbial characteristics or terms in complete sentences: psychrophile. obligate halophile. obligate aerobe.