You are making a potassium phosphate buffer that has 54 mM total phosphate (weighed out as KH2PO4), 205 mM KCI, and 7 mM dithiothreitol. You need 299 ml of buffer, which will be titrated to pH 54. What volume of a 25 mM dithiothreitol stock solution should you add to the flask before diluting it to the final volume for your buffer? Report the volume in ml to the nearest 1 mL.

Chemistry & Chemical Reactivity
9th Edition
ISBN:9781133949640
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter17: Principles Of Chemical Reactivity: Other Aspects Of Aqueous Equilibria
Section17.2: Controlling Ph: Buffer Solutions
Problem 2RC: 2. If an acetic acid/sodium acetate buffer solution is prepared from 100. mL of 0.10 M acetic acid...
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You are making a potassium phosphate buffer that has 54 mM total phosphate (weighed out as KH2PO4), 205
mM KCI, and 7 mM dithiothreitol. You need 299 ml of buffer, which will be titrated to pH 54. What volume of a 25
mM dithiothreitol stock solution should you add to the flask before diluting it to the final volume for your buffer?
Report the volume in ml to the nearest 1 ml.
Transcribed Image Text:You are making a potassium phosphate buffer that has 54 mM total phosphate (weighed out as KH2PO4), 205 mM KCI, and 7 mM dithiothreitol. You need 299 ml of buffer, which will be titrated to pH 54. What volume of a 25 mM dithiothreitol stock solution should you add to the flask before diluting it to the final volume for your buffer? Report the volume in ml to the nearest 1 ml.
At steady state
ES is made and ES dissociated, but the concentration of ES is effectively constant
ES is no longer formed and does not dissociate
ES is formed but dissociates immediately
there is no ES complex
Transcribed Image Text:At steady state ES is made and ES dissociated, but the concentration of ES is effectively constant ES is no longer formed and does not dissociate ES is formed but dissociates immediately there is no ES complex
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