You are studying a new operon that you have discovered in E. Coli that you have named the Late operon for its ability to help E. Coli grow and divide by feeding it Red Bull. You have
Q: Draw a picture illustrating the general structure of an operon and identify its parts.
A: BASIC INFORMATION OPERON The operon model was given by Francois Jacob and Jacques Monod. This…
Q: A researcher was trying to determine whether two molecules (molecule A and molecule B) were co-…
A: CoRepressor - The function of the corepressor is to induce transcriptional silencing or to repress…
Q: Describe operons in your own words, as if you were explaining what an operon is to someone who knew…
A: DNA is the biomolecule which contains all biological information in an organisms . the part of the…
Q: Explain why large amounts of transcript result when a bacterium with a lac Operon is in a medium…
A: Transcription is the process by which RNA is synthesized from DNA. The messenger RNA or mRNA…
Q: What turns the operon on when lactose is present?
A: An operon is defined as a functioning unit of DNA that contains a cluster or group of genes under…
Q: Compare and contrast the lac operon and trp operon of E. coli.
A: E.coli is a type of bacteria that is mostly found in the human intestine and gut of some animals. It…
Q: A mutation at the operator prevents the regulator protein from binding. What effect will this…
A: An operon includes a group of genes that have a common promoter, regulator, and would be transcribes…
Q: The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose…
A: The prokaryotic gene regulation is performed by the operon system. In this system the polycystronic…
Q: Compare and contrast the lac operon and repressible operon systems.
A: Expression of each and every gene is not always required in an organism. Gene expression must occur…
Q: E. coli has five genes that code for enzymes that make tryptophan. These genes are regulated by a…
A: When a group of related genes exist under the control of a single promoter, it is referred to as an…
Q: Which of the following is false when the genes of the Lac operon are being tran- scribed in high…
A: Lac operon is a set of genes used by E.coli to metabolize lactose when glucose is not available for…
Q: Define the term operon and explain one advantage it provides to a bacterial cell
A: The cells are the primary unit of life. Based on the number of cells an organism may be prokaryotic…
Q: A bacterial cell is mutated and cannot create the repressor protein associated with the Lac operon.…
A: Lac operon is associated with uptake and metabolism of lactose. It has several components such as a…
Q: The trp operon is a repressible operon system. What does this mean?
A: Trp refers to tryptophan. The operon is the functional unit of Deoxyribonucleic acid ( DNA) having…
Q: e following genetic and cellular conditions. You do not need to consider the role of CAP in your…
A: Upper operon - Is is super repressor. Once it is present in the sequence, it can not be removed. If…
Q: operon
A: Answer: Operon: In genetics, an operon is a functioning unit of DNA containing a cluster of genes…
Q: Study the illustration of the lac operon below. Normally, the genes are switched off when lactose is…
A: 1. When lactose is unavailable, the lac repressor binds strongly to the operator, inhibiting RNA…
Q: A bacterial cell has very low glucose and lactose is present. Explain how the lac operon will…
A: Lac Operon -- An operon is a functioning unit of genomic DNA which contains a group of genes…
Q: You are growing E. coli in a laboratory in order to study their operons. The growth media you are…
A: 1. What effect does lactose have on the bacterial cell’s lac operon? Answer:- It induces the operon,…
Q: Do you expect operons to occur extensively in Eukaryotes? Yes or no and explain.
A: Operons (clusters of co-regulated genes with related functions) are a well-known feature of…
Q: You are studying the ABCD operon from a newly isolated bacterium. Which of the following would…
A: A Operon consists of segments of DNA , which carry codes for the synthesis of proteins. These genes…
Q: E. coli has five genes that code for enzymes that make tryptophan. These genes are regulated by a…
A: Tryptophan operon found in E.coli and some other bacteria. It is group of genes which encodes for…
Q: I need help with answer 2 and 3
A: Ans. 2 promoter sequence
Q: Is the fox operon inducible or repressible?
A: Regulatory gene in the operon produces a protein which can act as either inhibitor or activator of…
Q: In E. coli, the trp operon is on by default, while the lac operon is off. Why do you think that this…
A:
Q: The transcription of many bacterial genes relies on functional groups called operons, such as the…
A: Turning/switching genes on and off is referred to as gene regulation. Cells start to take on…
Q: What is not a part of an operon?
A: Operon comprises all the genes that are involved in metabolic regulation. It is the unit of the gene…
Q: appropriate to use inducible operons? When is it appropriate to use repressible operons?
A: An operon is a collection of genes with similar functions that is managed by a single shared…
Q: A scientist has discovered a strain of bacteria that can convert ethanol to pyruvate when stimulated…
A: Solution 1) The repressor of this operon is functional It is active when ethanol is absent and…
Q: when tryptophan is present, what would happen to trp operon regulation
A: Introduction:- The trp operon is a collection of genes identified in E. coli bacteria that code for…
Q: For an enzyme to be produced from an inducible operon, the repressor must bind to and digest the…
A: The operon is defined as a set of genes transcribed together and is regulated by one promoter. The…
Q: The tryptophan operon is regulated by two methods. Describe in detail the regulation of the operon…
A: Genes are the functional unit of heredity. Operons are a group of genes under a single promoter. The…
Q: A mutation at the operator prevents the regulator protein from bindingWhat effect will this mutation…
A: A group of genes that are transcribed under a single promoter and an operator is referred to as…
Q: You are studying a bacterial plasmid that contains 3 operons and 9 genes. How many transcriptional…
A: In bacteria, similar and related genes are often found in a cluster on the chromosome, where they…
Q: The lac operon has which of the following characteristics? O 1) usually requires an activator…
A: In Eukaryotes all genes are separate and produce individual mRNAs on transcription. However, in…
Q: What is an operon? Describe the functions of regulators, promoters,and operators.
A: The structure responsible for the regulation of genes in order to synthesize the associated proteins…
Q: Would you expect to see attenuation in the lac operon and other operons that control the metabolism…
A: Lac operon contains genes that are involved in the lactose metabolism. It is expressed when lactose…
Q: The lac operon is an inducible operon. What does this mean?
A: Operons are the groups of genes related to a single pathway and controlled by a single promoter.…
Q: explain how the Lac operon gets turned on and off .
A: Introduction :- Three structural genes, as well as a promoter, a terminator, a regulator, and an…
Q: The trp operon is a repressible operon. A strain of e coli develops a mutation whereby the trp…
A: Repressible genes continues to express themselves till a chemical ( often an end product) inhibits…
Q: You have E. coli growing in HIGH lactose and HIGH glucose. You measure the amounts of protein and…
A: The E.coli is growing in a medium rich in lactose and Glucose both. There are two regulatory…
Q: Explain the steps in Trp Operon. Take note, important enzymes and chemicals are needed in your…
A: The trp operon is a group of genes that codes for the molecules that are responsible for the…
Q: Describe in details all the differences between The Trp Operon and the Lac Operon. Why one is always…
A: As operon is that the cluster of genes expressed along from single promoter and lac and trp area…
Q: If a particular operon encodes enzymes for making an essential amino acid is regulated like the trp…
A: The operon has five structural genes that code for the three enzymes required to convert chorismic…
Q: What is operon? How does the lac operon work?
A: A specific sequence of nucleotides in DNA is called DNA. It is a hereditary unit which is…
Q: A mutation occurs in the 5′ UTR of the trp operon that reduces the ability of region 2 to pair with…
A: In the absence of tryptophan, the trp operon would be expressed maximum as the trp repressor would…
Q: A mutation at the operator prevents the regulator protein from binding.What effect will this…
A: An operon is defined as a group of genes that have a common promoter and regulator and also…
Q: E. coli is given lactose as a nutrient source. This results in the activation of the lac operon only…
A: The solution to the above mentioned questions is as follows:
Q: Explain the steps in Lac Operon. Take note, important enzymes and chemicals are needed in your…
A: Operon is a coordinated group of genes (structural , operator & regulator ) that is designed to…
Q: What is the difference between the role of the operator and the role of the promoter in an operon
A: According to Jacob and monod concept, operon is a group of contiguous structural genes showing…
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- Let’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively expressed. In other words, the operon is turned on in the presence or absence of lactose. One possibility is that the mutation may block the transcription of the lacI gene, thereby preventing the synthesis of lac repressor. A second possibility is that the mutation could alter the sequence of the lac operon in a way that prevents the repressor protein from binding to the operator. How would you distinguish between these two possibilities?You have isolated different mutants (reg1 and reg2) causing constitutive expression of the emu operon (which has genes emu1 and emu2). One mutant contains a defect in a DNA-binding site, and the other has a loss-of-function defect in the gene encoding a protein that binds to the site Say you don’t know which mutant has a defect in the site and which one has a mutation in the binding protein. To figure it out, you construct the two partial diploid strains (i and ii below), and you then assay the levels of the Emu1 and Emu2 proteins in these two strains. F’ (reg1- reg2+ emu1- emu2+) / reg1+ reg2+ emu1+ emu2- F’ (reg1+ reg2- emu1- emu2+) / reg1+ reg2+ emu1+ emu2- What proteins do you predict will be expressed for strains i and ii if reg2 encodes the regulatory protein and reg1 is the regulatory site?In addition to observing similarities to the lac operon, you also notice that this gene is regulated via attenuation, similar to the trp operon. Based on this similarity to this model operon, you could state that ___________. Group of answer choices If a terminator loop forms in the DNA, the expression of the structural genes is halted. The formation of the terminator hairpin followed by a series of Uracil (UUUUUUU) functions similar to Rho-Independent termination to result in the stopping of transcription. The transcription of a leader sequence affects the translation of the structural genes. Never mind – all of these statements are true! Attenuation will be the primary means of transcriptional regulation, with a repressor used as a back up option.
- Suppose you are studying the regulation of a gene involved in the metabolism of two nutrients, Llamasin and Alpacalon, in bacteria. You are trying to determine if these nutrients act as inducers in their operons. The following data were collected from your experiments. Which of these operons is most similar to the lac operon? Explain. Nutrient Levels of nutrient in growth medium Level of transcription of genes in operon Llamasin low high high low Alpacalon low low high high Highlight one in green: Llamasin Alpacalon Explanation:Consider the trp operon found in E. coli. You have discovered a mutant strain of E.coli that contains a mutation in its trpR gene. This mutation prevents the trpR protein from binding to its small effector molecule. What would happen if both the wild type and mutant bacteria were put in an environment that contained lots of tryptophan? The normal (wild type) bacteria would have (i)________expression of the trp operon while the mutant bacteria would have (ii) _________ expression of the trp operon. A) (i) high (ii) high B) (i) low (ii) low C) (i) high (ii) low D) (i) low (ii) highYou are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions using the dropdown menus provided in Canvas. Which operons would be functional under these conditions? What repressors would be made? (NOTE: name the repressors using their gene names) Which repressor(s) would be made in the inactive form? Which repressor(s) would be made in the active form? Which repressor(s) under these conditions can bind the operator sequence?
- You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions using the dropdown menus provided in Canvas. Which operons would be functional under these conditions? What repressors would be made? (NOTE: name the repressors using their gene names) Which repressor(s) would be made in the inactive form? Which repressor(s) would be made in the active form? Which repressor(s) under these conditions can bind the operator sequence? Which repressor(s)under these conditions cannot bind the operator sequence? What effect does lactose have on the bacterial cell’s lac operon? What does the absence of glucose do to the bacterial cell? You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? What kind of regulation does lactose provide to an operon’s…To study the lac operon, you engineer a strain of E coli to have a lac operon in which the lac Z gene is replaced by the gene for green fluorescent protein (GFP). Expression of GFP generates a green color in the cells that can be easily quantitated with a fluorescence microscope. You test the activity of the operon in the absence of the inducer IPTG, the presence of the inducer IPTG and the presence of an antibiotic the completely inhibits RNA polymerase (i.e. no gene expression). You then use this system to test the effects of various mutation on the activity of the operon. Match the following mutations with the activity (A, B or C) you would expect to observe with the mutation. All experiments are done in the presence of IPTG unless otherwise stated.You then make a screen to identify potential mutants (shown as * in the diagram) that are able to constitutively activate Up Late operon in the absence of Red Bull and those that are not able to facilitate E. Coli growth even when fed Red Bull. You find that each class of mutations localize separately to two separate regions. For those mutations that prevent growth even when fed Red Bull are all clustered upstream of the core promoter around -50 bp. For those mutations that are able to constitutively activate the operon in the absence of Red Bull are all located between the coding region of sleep and wings. Further analysis of each DNA sequence shows that the sequence upstream of the promoter binds the protein wings and the region between the coding sequence of sleep and wings binds the protein sleep. When the DNA sequence of each is mutated, the ability to bind DNA is lost. Propose a final method of gene regulation of the Up Lateoperon using an updated drawn figure of the Up Late…
- Bacterial DNA containing an operon encoding three enzymes is introduced into chromosomal DNA in yeast (a eukaryote) in such a way that it is properly flanked by a promoter and a transcriptional terminator. The bacterial DNA is transcribed and the RNA correctly processed, but only the protein nearest the promoter is produced. Can you suggest why?If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?