You have a 20% stock of NaCl, a 30% stack of KHPO, 2.5 M NH CI and 10 mg/ml stock of ampicill a. How many grams of NaCl would you need to weigh out to make up 250 ml of a solution? 20
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- 2 if there is a stock solution of 2000 (ug/ml) , is it possible to use SERIAL DILUTION to generate the following 8 tubes with their concentrations? A 2000 (ug/ml) B 1500 (ug/ml) C 1000 (ug/ml) D 750 (ug/ml) E 500 (ug/ml) F 250 (ug/ml) G 125 (ug/ml) H 0 (ug/ml)You have 12 mL of cells you need to treat with Hydrogen Peroxide (H2O2), such that the final concentration of H2O2 is 50 uM. How much of a 30 mM stock solution of H2O2 would you add to your cells?Specific rotation of L-alanine [(+)alanine) in water (at 25 °C) is +2.8. Suppose you have 2.00 g of mixture of L and D-alanine [(-)alanine]. You dissolved it in 10 mL of water, and measured rotation in 10 cm cell. The observed rotation was +0.12. Calculate the ee% for the mixture. How many grams of D- and L-alanine do you have in the sample?
- A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show workingYou want to treat 10 mL of HL-60 cells with cycloheximide in order to determine the half-life of a newly discovered protein. In order to do this, the final concentration of cycloheximide needs to be 90 µM. How much of a 150 mM stock solution of cycloheximide should you add to your cells?A 40 umol.l-1 solution of flavin mononucleotide (FMN) gave an absorbance of 0.8 at 470 nm using a 1 cm cuvette. What is the molar extinction coefficient of FMN in M-1 cm-1?
- Given a tripeptide Cys-His-Lys, Cys: Pk1 = 1.5; Pk2 = 10.8; PkR = 8.5 His: Pk1 = 1.6; Pk2 = 9.0; PkR = 7.0 Lys: Pk1 = 2.2; Pk2 = 8.5; PkR = 9.8 a.draw the protonic equilibria for the tripeptide. b.what is the IpH? c.What is the dominant structure at pH 3.0? d.What is the first buffering region of the tripeptide?1. You want to purify a protein using anion exchange column chromatography. In this technique, the solid state beads are positively charged. What charge would you want your protein to have in order for it to "stick" to the beads? Neutral Positive Negative 2. In your anion exchange experiment, your protein of interest has a pI of 6.0. Which of the following buffer solutions would you want to use when loading your protein extract onto the column so that your protein "sticks" to the beads? pH 4.5 pH 7.3 pH 6.0 3. You want to analyze your chromatography results using gel electrophoresis. If your protein is a pentamer consisting of 2 alpha subunits, 1 beta subunit, and 2 gamma subunits, how many bands would you expect to see on your gel? The alpha subunits are 32kDa. The beta subunit is 32kDa. The gamma subunits are 15kDa. 1 5 2 3 4. You want to analyze your chromatography results using gel electrophoresis. If your protein is a pentamer…1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.
- 3. a) An aqueous solution is 3.50% by mass potassium bromide, KBr, and has a density of 1.02 g/mL. The molality of potassium bromide in the solution is _________m. b)An aqueous solution of magnesium nitrate has a concentration of 0.474 molal. The percent by mass of magnesium nitrate in the solution is _________%. c)An aqueous solution of cobalt(II) bromide has a concentration of 0.153 molal. The percent by mass of cobalt(II) bromide in the solution is _________%.You have a 1000X stock solution of sodium chloride (NaCl). The concentration of NaCl in the stock solution is 5M. a)How would you make 300mL of a 1X solution? b)What is the concentration of NaCl in your 1X solution?If the absorbance at540nm of a biuret and protein solution containing 0.25mg/ml of protein .24 the absorbance of a biuret and protein and dna solution containing.25mg/ml of protein and 0.5mg/ml dna should be note the total volume is the same in both solutions 0.24 0.72 0.48 not enough infor