You need to prepare an acetate buffer of pH 5.43 from a 0.621 M acetic acid solution and a 2.95 M KOH solution. If you have 730 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 5.43? The pKa of acetic acid is 4.76. Be sure to use appropriate significant figures.
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- To make up a solution of phosphate buffered saline (PBS), you need 10 mM Na2HPO4 (anhydrous) (FW: 141.96 g/mol), 3 M NaCl (FW: 58.44 g/mol), and 5mM KH2PO4 (FW: 136.09 g/mol). How many grams of each will you need to make up 850 mL of PBS?Three buffers are made by combining a 1M solution of acetic acid and a 1M solution of sodium acetate in the ratios shown in the table below. Which of these statements is true regarding the prepared buffers? (Ka= 1.7x10-5) pH of buffer 1 < pH of buffer 2 < pH of buffer 3 pH of buffer 1 = pH of buffer 2 = pH of buffer 3 pH of buffer 1 > pH of buffer 2 > pH of buffer 3 pH of buffer 1 = pH of buffer 2 > pH of buffer 3 pH of buffer 1 > pH of buffer 2 = pH of buffer 3How much of a 5x buffer and how much water should be added to make 1 L of a 1x solution?
- If 4 mL of 1 M NaOH is added to 100 mL buffer, would it still be a usable buffer according to the conventions? Explain why or why not.What is the pH of the following buffer mixtures? (a) 100 mL 1 M acetic acid plus 100 mL 0.5 M sodium acetate (b) 250 mL 0.3 M phosphoric acid plus 250 mL 0.8 M KH2PO4What is the pH of a solution of 100 ml of 0.01 M H3PO4 and 100 ml of 0.01 M Na3PO4?
- In order to make a solution of the concentration 0.364 M, what mass of NaCl should bedissolved in 152 mL of water?Calculate pH of buffer prepared by mixing; 10ml of 0.10M of CH3COOH and 20ml of 0.10M of CH3COONa.TE buffer consists of 10 mM of Tris-Cl, pH 7.6 and 1mM of EDTA, pH 8.0. You need to prepare 3L of TE buffer and you have the following stock solutions: 500 mM Tris-Cl and 1 M EDTA. How will you prepare the 3L of TE? Write work clearly