You are preparing 1 Lt of TAE (Tris Acetate EDTA) gel running buffer from a 50x stock solution. How much 50 x TAE and how much water will you combine?

Q: What is the strength (mg/mL) of promethazine hydrochloride in the following compound? How many…

A: Promethazine hydrochloride is the first generation antihistamine. It is used to treat allergies,…

Q: The protein concentration of a known standard is 100mg/mL If you prepared a serial dilution, mixing…

A: The process of reducing the concentration of a solution by adding more solvent is known as dilution.…

Q: Sodium Citrate 5 g Tartar Emetic 0.015 g Cherry Syrup ad 120 mL Using a balance with a sensitivity…

A: Correct answer is 22.5 ml  Examplnation given with image in step 2

Q: You have a 62% dextrose solution. How many mL will give you 35 g of dextrose?

A: Carbohydrates are molecules that include carbon, hydrogen, and oxygen and have the formula (CH2O)n.…

Q: if you had a protein sample solution of unknown concentration which gave an absorbance of 0.992…

A: Answer:- The standard curve gives the absorption value of aliquots having the substance of known…

Q: hat mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to…

A: We have given [ C2 H4 O3  = 1.00 , Volume= 4.00mL, pH=4.0

Q: Given an 8 M potassium chloride stock solution, explain whether it is possible to perform a dilution…

A: The dissolution of one mole of a compound or solute in a solution with a total volume of one litre…

Q: You have a 1000X stock solution of sodium chloride (NaCl). The concentration of NaCl in the stock…

A: Sodium chloride (NaCl) is a salt made of an equal composition of sodium and chloride ions. NaCl is…

Q: An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper…

A: Paper chromatography is the analytical process by which mixtures of complex compounds such as amino…

Q: Potentiometric titration curve is given below, which is obtained during the potentiometric titration…

A: Asked : Question related to the shown titration curve x,y represent Volume of KOH, pH of solution…

Q: If you were presented with 2 L of a 2 M sucrose stock solution, how many grams of sugar would be in…

A: Molarity: The moles of a solute per liter of a solution is called as molarity (M).

Q: How will you prepare a 5% BSA solution? Show all your steps.

A: BSA stands for bovine serum albumin, which is a protein extracted from cows' serum albumin. In lab…

Q: 50 microliters of your original sample to the Bradford reagent. What is the concentration of your…

A: Proteins are made up of amino acids that have been polymerized. As a result, amino acids are…

Q: Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66,…

A: According to the question, we need to identify the three amino acids that were present in the given…

Q: You are asked to prepare 50 ml of a 1:100 rifampin (rifadin) and you have in stock a 1:20 rifampin…

A: Rifampin (Rifadin) is an antibiotic used to treat bacterial infections like leprosy, tuberculosis…

Q: A 500 mL sample of a 0.100 M formate buffer, pH 3.75, is treated with 5 mL of 1.00 M KOH. What is…

A: The buffer solution consists of an acid and a salt of the conjugate base of the acid in a solution.…

Q: Tomato juice has a OH- concentration equal to 1 X 10 -10, what is the pH of this solution?

A: In a solution, the negative log of the concentration of hydrogen ion denotes the pH and the negative…

Q: The pH of a 0.0082 M solution of HNO₃ is

A: The pH of solution is a measure of the molar concentration of hydrogen ions in the solution and as…

Q: Calculate how many grams of agarose you would need to prepare a 125 mls of 1.2% gel? O 12 grams O…

A: Here we need to prepare a 125ml of 1.2% gel agarose. 1.2% means 1.2 grams in 100 ml. But we need…

Q: You have 10 mg/ml ethidium bromide solution. If you add 5 µl of this to 50 ml of agarose gel…

A: Gel electrophoresis is a laboratory method for separating DNA molecules depending on their size and…

Q: 1. How    do    you    prepare    25    mL    of    a    0.4X    solution    of    SDS    from    a…

A: Using formula C1V1= C2V2, we can calculate the Molarity, w/v, %, or any other concentration of the…

Q: 3. A 30.0-ml. sample of 0.20 M HA was titrated with 0.20 M NAOH. The following data were collected…

A: Given Values: Concentration of HA = 0.2 M Volume of HA = 30 ml = 0.03 L Concentration of NaOH = 0.2…

Q: There is An order is received for 10 fl oz of a 5% solution. In stock is a 10% solution. How many…

A: Introduction:-  Initial concentration of solution is 25% and we have to make it 5% 300ml solution.…

Q: You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing…

A: In biochemistry, a stock solution is a high volume of a standard reagent. They're utilized for a…

Q: To prepare 500 mL of a 1X TAE buffer solution, we need to mix solution (so 25 times more…

A: TAE is a standard buffer for the preparation and operation of DNA agarose gels. TBE provides…

Q: How would you make wash buffer with 0.05M Tris containing 0.05M NaCl in 200ml at pH of 8.0

A: A solution is a mixture of one or more than one solute mixed in a solvent. The molarity of the…

Q: An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper…

A: Paper chromatography is the analytical process by which mixtures of complex compounds such as amino…

Q: You need to prepare 1L of a 5×TE stock solution (1×TE buffer:10mM Tris,1mM EDTA).How much of Tris…

A: The mole is used as a unit in the SI system to quantify the amount of substance present. One mole is…

Q: How many milliliters of 0.1M aqueous Sodium Hydroxide solution can be prepared from 10 gm ?of Sodium…

A: The molarity (M) is the amount of a substance dissolved in a certain volume of a solution. It is…

Q: You have a 20% stock of NaCl, a 30% stack of KHPO, 2.5 M NH CI and 10 mg/ml stock of ampicill a. How…

A: The total number of shares that are literally listed (bought and sold) throughout the business day…

Q: How much agarose powder (g) would you use to make a 50 ml, 1.4% agarose gel?

A: Introduction:- Agarose powder is a linear polysaccharide that forms a gel when it is mixed with…

Q: A pharmacist receives a prescription for 60 g of a 0.75% w/w bexarotene gel. E many grams each of a…

A: Pharmacology is a discipline of science concerned with the study of medications and their effects on…

Q: Suppose you wanted to make a buffer of exactly pH 7.00 using KH2PO4 and Na2HPO4. If the final…

A: The buffer solution is a solution which resists any change in pH on addition of small amounts of…

Q: Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration…

A: The following formula is used to find the volume required to prepare a 5 mL sample with a final…

Q: You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis.…

A: TAE buffer used in agarose gel electrophoresis for the seperation of DNA and RNA.

Q: An aqueous solution contains 0.448 M dimethylamine ((CH3)2NH). How many mL of 0.240 M hydroiodic…

A: Buffer solution contains a mixture of weak acid and its conjugate base or weak base and its…

Q: How many moles of KBr are there in 89.5 mL of 0.150 M KBr?

A: Molarity can be stated as, it is the number of moles of solute which is dissolved per liter (L) in…

Q: How would I calculate the amount of each component in each solution?  Stock solutions provided:…

A: In-order to perform dilution, we take a specific volume from stock solution and transfer it to…

Q: TE buffer consists of 10 mM of Tris-Cl, pH 7.6 and 1mM of EDTA, pH 8.0. You need to prepare 3L of TE…

A: Given Values: The concentration of the stock solution of Tris-Cl = 500 mM The concentration of the…

Q: f a 250 ml solution of ethanol in water is prepared with 4 ml of absolute ethanol (100%), what is…

A: Dilution factor  It is defined as the ratio of the initial volume of a solute to the final volume…

Q: How would you prepare 3L of a basic buffer having a pH of 8.6 where the concentration of base is…

A: A buffer solution is any solution that is a mixture of weak base and its conjugate acid or…

Q: You are asked to make a 5M solution of SO 2, how many grams do you need?

A: A solution is a homogenous mixture that is homogenous. It is made up of two substances or more…

Q: Why do we need to place the lipid samples under direct heat after adding ethanol in a solubility…

A: The solubility test determines the presence of all lipids as a preliminary test.

Q: Which lipid sample (butter/ oil) is soluble or miscible in 70% ethanol? Explain. Do you think the…

A: Solubility and miscibility are mostly interchangeably used because the resultant solution seems to…

Q: if you perform a 1:5 dilution of a 1 M solution , how many parts of the 1 M solution do you use, and…

A: Dilution factor describes  ratio of aliquot volume to final volume. It is a notation often used in…

Q: Explain how you would prepare 100ml of 10mMProline solution given that the molecular mass of…

A: Proline : It is an amino acid, plays an important role in plants. It protects the plants from…

Q: We have 50 X TAE (Tris-Acetate) stock in our laboratory and the laboratory manager asks you to…

A: TAE buffer is a common buffer used in DNA electrophoresis. It has a pH of 8.0 and is made up of Tris…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.