You have two DNA samples, A and B. A 1/10 dilution of sample A had an absorbance at 260nm of 0.12. A 1/25 dilution of sample B had an absorbance at 260nm of 0.12. 5µL of the undiluted sample A and 5uL of the undiluted sample B were added to 90 µl of water. What is the final DNA concentration in ug/µL?
Q: Compute the concentration of the double-stranded DNA using the formula: dsDNA concentration = 50…
A: Introduction Spectrophotometric analysis is very important for measuring the concentrations. It is…
Q: You have 10 ul of DNA you want to cut with HindIII, and then run a gel, in order to cut out certain…
A: Restriction Digestion is a process by which DNA molecules are cut into small pieces with the aid of…
Q: A plot showing the % of denaturation as a function of temperature for a melting point of a DNA…
A: DNA is a double stranded helically wound macromolecule. Each strand is made up of a sequence of…
Q: In primer designing, which of the following statements is correct? a. Primers should be 18-24 bases…
A: Primer is a short synthetic oligonucleotide which is used in many molecular techniques from PCR to…
Q: The absorbance of a nucleic acid sample at 260 and 280 nm are 0.4857 and 0.2427, respectively. Based…
A: The UV absorbance method works on the idea that nucleic acids (DNA or RNA) have conjugated double…
Q: Which of the following equations are true for double-stranded DNA? a) (%G+%T) =(%A+%C) b) (%G+%A)…
A: DNA stands for deoxyribonucleic acid. It is a genetic material that passes from one generation to…
Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…
A: Given values: Total DNA extracted = 500 μL Total weight of wheat used for DNA extraction = 200 mg…
Q: when measuring the purity of a DNA sample? Please select the single answer that is most correct.
A: An instrument called spectrophotometer is able to measure the absorbance of specific wavelength of…
Q: Which of the following is most needed to determine the DNA quantity using the eyeball method? A DNA…
A: DNA quantity was determined by several methods where absorbance was one of the widely used methods…
Q: I have a 1.270 ug/uL dna stock, how would you dilute this to 50ng/nL in a single dilution step
A: Formula - 1 ug = 1000 ng Dilution = initial volume / final volume = final concentration / initial…
Q: "Chargaff's rules" about the composition of bases in DNA dictates that A. the sum of purine…
A: Chargaff's rules explain the structure and composition of DNA. Chargaff's rules include the…
Q: The amount of G plus C in an organism’s DNA is 64% of the total base content of that DNA. What are…
A: DNA (deoxyribonucleic acid) is that the genetic material present in archaea, eubacteria,…
Q: In a sample dsDNA from an organism, the amount of thymine is analyzed to be 12 pmoles when the…
A: ds DNA :-The anti-dsDNA (anti-double stranded DNA) antibody test is a blood test that looks for…
Q: nucleotide base pairs found in DNA?
A: The genetic information storing molecules are nucleic acids. These are polymers of nucleotides. A…
Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…
A: Biotechnology is the use of our understanding of biological processes to develop useful applications…
Q: How much buffer would you need to dilute a stock solution of DNA that is at a concentration of…
A: We will use following equation in this case: C1× V1 = C2 × V2 Here C1 is the stock concentration V1…
Q: An analysis of the bases in DNA present in body cells gave the following results: W units/g X…
A: DNA is a double stranded molecule which is a polymer of nucleotides. The components of nucleotides…
Q: Compared to a 3% agarose gel, how far should the same size DNA travel in a 1% agarose gel? not as…
A: The DNA has a uniform mass proportion, DNA particles are isolated by size inside an agarose gel in…
Q: Which of the following equations is a prediction based on Chargaff’s rules for the content of DNA?…
A: Chargaff's rule says that DNA should have 1:1 ratio of pyrimidines and purine bases. According to…
Q: IF A DOUBLE-STRAND DNA SAMPLE WAS COMPOSED OF 10 PERCENT THYMINE, WHAT WOULD BE THE PERCENTAGE OF…
A: The deoxyribonucleic acid is the genetic material that is passed from one generation to the next…
Q: In your forensic laboratory, you set up a reaction to digest DNA with restriction enzymes. The final…
A: In molecular cloning techniques like PCR and restriction cloning, restriction enzyme digestion is…
Q: The table below shows the percentage of the nitrogenous bases present in each sample. Which of the…
A: The nitrogenous bases are the part of nucleotide the nucleic acid. A, T, G and C are four…
Q: The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 109 In your answers,…
A: The bacterium Escherichia coli, commonly known as E. coli belongs to the family of gram negative…
Q: The "D" in DNA stands for which of the following?
A: DNA : Chemical name for molecule which carries genetic instructions in the living organisms.
Q: You have a 1.270 ug/uL DNA stock. What volume will give you 10 ug?
A: In this question, we are given a concentration of DNA that is 1.270 ug/ul. We have to determine the…
Q: Chargaff's Rule implies the percentage of adenine-thymine pairs and the percentage of…
A: DNA in eukaryotes is double stranded linear molecule. It is composed of adenine, guanine, cytosine…
Q: Why does DNA appear when you add cold alcohol to the strawberry DNA solution?
A:
Q: Chargaff’s results yielded a molar ratio of 1.29 for A to G in ox DNA, 1.43 for T to C, 1.04 for A…
A: DNA (deoxyribonucleic acid) is the genetic material of an organism. The DNA is passed on from the…
Q: Why does the absorbance increase when a DNA sample unwinds?
A: DNA is considered as the double (ds) stranded molecule, which joined together by the H-bond between…
Q: If you tiled a floor with the planar rings from the bases of all the DNA molecules of living…
A: DNA, or deoxyribonucleic acid, is the hereditary material in humans located in the cell nucleus. It…
Q: You have a 2.5 ng/uL DNA stock. What volume will give you 1.15 mg?
A: Conversion tables are important to find out the actual values in the required unit. Similar…
Q: If you have G-C rich DNA versus A-T rich DNA, a. will the melting temperature be the same? Why b.…
A: DNA or deoxyribonucleic acid is defined as the molecule that will consist of the organism's genetic…
Q: 100 F В A 75 80 85 90 95 100 105 110 115 120 125 Temperature (°C) A) What is the tm of the most…
A: DNA is a double-stranded helical molecule. The monomer of DNA is a nucleotide. A nucleotide…
Q: Using a 1 cm cuvette, the absorbance at 260nm of your double-stranded DNA sample is 0.15. What is…
A: The concentration of DNA in a sample can be estimated by different methods - absorbance (optical…
Q: What is the purpose of Bromophenol Blue in electrophoresis? a. To follow the progress of the gel as…
A: Introduction The technique of gel electrophoresis is used to separate DNA fragments based on their…
Q: QUESTION 1 For the DNA structure below write the correct base sequence in the form: 5'-ATCT-3…
A: Question 1. Nucleic acids are the genetic material of the cell and are composed of recurring…
Q: Explain how to prepare 2 ml of a solution with a concentration of 1 μg/5ml from a stock solution of…
A: Given- Stock solution of DNA sample- 0.1 mg/ml Volume required= 2 ml Concentration of the DNA stock…
Q: Use Chargaffs Rule to determine the percentage of base T in a sample of DNA that is 28% A and 22% C.…
A: Chargaff observations on the base pairs lead to some rules that help calculate the percentage of…
Q: The 260/280 ratio of pure DNA is between 1.8- 35 2.0. The absorbance of DNA at different 30…
A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…
Q: DNA bands from each sample lane of the gel electropherogram below.
A: Key points: Gel electrophoresis is a technique used to separate DNA fragments according to their…
Q: The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 109 In your answers,…
A: DNA is a double-helix structure that is made up of two intertwined polynucleotide strands that are a…
Q: n a pUC19 digest for 1 ug of pUC19 (DNA conc. 282ng/ul) using 10X Cutsmart buffer, pure water and…
A: Restriction digestion is a process by which restriction endonuclease (eg. BamHI) cuts the DNA at a…
Q: From the complete genome sequence of an organism, you have determined the following percentages of…
A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…
Q: The nucleotides within DNA are composed of a Group of answer choices A sulfur group, ribose sugar,…
A: DNA stands for Deoxyribose Nucleic Acid. The strands of DNA are made up of nucleotides.
Q: A DNA molecule consists of 500 nitrogenous bases, of which 150 are G bases. So, the percentage of…
A: DNA molecule is the genetic material present in the animals. Genetic material is present in the…
Q: The image below is an example of the results that you could expect to see from your gel. Each group…
A: PCR amplification of a gene and detecting the amplified product on agarose gel is very common…
Trending now
This is a popular solution!
Step by step
Solved in 6 steps with 3 images
- Using a 1 cm cuvette, the absorbance at 260nm of your double-stranded DNA sample is 0.15. What is the concentration of your sample?You have a 20 mg/ml of Ethidium bromide stock solution. You need to a final concentration of 2ug/ml into a Agarose solution to visualize DNA. What is the dilution factor? Give typing answer with explanation and conclusionCompute for the appropriate amount (mL_) of the compositions of a CTAB NaCl solution for DNA extraction. a. 10% (w/v) CTAB b. 0.7 M NaCl
- What is the concentration of a DNA solution with an OD260 measurement of 3.175 if 1OD unit at 260nm represents a DNA concentration of 50 g/mL? Write the correct units.The absorbance of a nucleic acid sample at 260 and 280 nm are 0.4857 and 0.2427, respectively. Based on the results, the sample is a. pure DNA b. pure RNA c. DNA contaminated with RNA d. DNA contaminated with proteinTo carry out sequencing, you need to include 600 ng of DNA and a total of 6.4 pmol of sequencing primer. If your concentration of DNA (determined by A260 reading on a Nanodrop Spectrophotometer) is 89.0 ng/μL, how many μL of this sample do you need for a total of 600 ng?
- How much 6x loading dye should be added to 49 uL of DNA? Report you answer in units of uL, with one digit after the decimal point.I have a 1.270 ug/uL dna stock, how would you dilute this to 50ng/nL in a single dilution step? show the exact process including the volume of water. Indicate if you would use p20,p200,p1000 for each reagant added. you can choose a total volume but cannot be less than 3 uL or exceed 1000uL. Thank you!!The concentration of digested and cleaned DNA is found to be 5μg/mL. How much of your DNA sample (in μL) would require to obtain a final concentration of 30ng.
- The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 10 9 D. In your answers, show how you came up to each result?(a) How many base pairs does this bacterium contain? (b) How many full double-helical turns does this DNA contain? (c) How long is this DNA in micrometer?Show the calculations for the preparation of 0.01%, 0.001%, 0.0001%, 0.00001% standard DNA solutions via serial dilution from a 0.1% stock solution. Prepare 10.0 mL of 0.10% standard DNA solution using acetate buffer pH 4.6 as solvent. Prepare four different concentrations of the 0.10% standard DNA solution from step 1 via serial dilution to obtain 0.01%, 0.001%, 0.0001%, 0.00001% standard DNA solutions. Obtain seven clean and dry test tubes. Place 1.50 mL solutions in each tube as follows: Table 2.1. Set-up for the diphenylamine assay. Test tube # Content 1 (blank) acetate buffer pH 4.6 2 0.10% standard DNA 3 0.01% standard DNA 4 0.001% standard DNA 5 0.0001% standard DNA 6 0.00001% standard DNA 7 DNA extract from Part I Add 3.50 mL diphenylamine reagent to each tube, swirl each tube to thoroughly mix the contents and heat for 10 mins in a boiling water bath. Cool immediately under tap water. Read absorbances at 595 nm.…In analyzing the base composition of a DNA sample, a student loses the information on pyrimidine content. The purine content is A = 27% and G = 23%. Using Chargaffs rule, reconstruct the missing data and list the base composition of the DNA sample.