How much 6x loading dye should be added to 49 uL of DNA? Report you answer in units of uL, with one digit after the decimal point.
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How much 6x loading dye should be added to 49 uL of DNA? Report you answer in units of uL, with one digit after the decimal point.
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- If the length of E.coli DNA is 1.36 mm, calculate the number of base pairs it containsUsing a 1 cm cuvette, the absorbance at 260nm of your double-stranded DNA sample is 0.15. What is the concentration of your sample?Make 2 mL of 50 fold dilution of DNA solution and sodium phosphate buffer. DNA: 400 uL Sodium phosphate buffer, pH 6.9, 10mL - Calclate amount of DNA & buffer need to make dilution
- A DNA sample contains 21% adenine. What is the complete percentage base composition?What is the concentration of a DNA solution with an OD260 measurement of 3.175 if 1OD unit at 260nm represents a DNA concentration of 50 g/mL? Write the correct units.What does the 260 nm light detect when measuring the purity of a DNA sample? Please select the single answer that is most correct.
- How many uL of DNA would I load into my gel if I am given a stock of 450ug/mL and I want to load 4ug?You have a 20 mg/ml of Ethidium bromide stock solution. You need to a final concentration of 2ug/ml into a Agarose solution to visualize DNA. What is the dilution factor? Give typing answer with explanation and conclusionWhat is the structure of the gel stain being used? There are several other dyes that can bind DNA and used for gel imaging. Find an example of such a dye, and draw its structure.
- When you measured your DNA preparation concentration using the NanoDrop spectrophotometer, it indicated that the DNA concentration was 0.550 ng/uL. Convert this concentration into ug/mL.The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 109 In your answers, show how you came up to each result? How long is this DNA in micrometer (μm)IF A DOUBLE-STRAND DNA SAMPLE WAS COMPOSED OF 10 PERCENT THYMINE, WHAT WOULD BE THE PERCENTAGE OF GUANINE?