You need to determine where your gene of interest lies on the bacterial chromosome. In order to accomplish this you mix a strain of bacteria that is leu- bio- pro- strR with a wild type Hfr strain. You already know that the pro gene is transferred after bio and leu. Based on this information and the table below what is the order of these three genes and what is the distance between each locus? At the end of your mapping experiment the following exconjugants were recovered: Exconjugant Genotype Number of exconjugants pro+ bio+ leu- 425 pro+ bio- leu- 62 pro- bio- leu 13 pro- bio leu- 250
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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