Crosses are carried out between a bacterial F- strain and three different Hfr strains. In these strains, it takes 60 minutes for an Hfr strain to transfer the entire chromosome. Below are the data generated from interrupted mating experiments. Time of transfer of different genes (in minutes) is given for each strain. Appearance of genes in F- cells Hfr1: Genes b+ d+ c+ f+ g+ Time 3 5 16 27 59 Hfr2: Genes e+ f+ c+ d+ b+ Time 6 24 35 46 48 Hfr3 Genes d+ c+ f+ e+ g+ Time 4 15 26 44 58 Based on these data, you could conclude that Group of answer choices b is between g and d and is 4 minutes from g e is between f and g and is 25 minutes from g g is between e and b and is 24 minutes from e none of these is correct d is between b and c and is 10 minutes from b

Crosses are carried out between a bacterial F- strain and three different Hfr strains. In these strains, it takes 60 minutes for an Hfr strain to transfer the entire chromosome. Below are the data generated from interrupted mating experiments. Time of transfer of different genes (in minutes) is given for each strain. Appearance of genes in F- cells Hfr1: Genes b+ d+ c+ f+ g+ Time 3 5 16 27 59 Hfr2: Genes e+ f+ c+ d+ b+ Time 6 24 35 46 48 Hfr3 Genes d+ c+ f+ e+ g+ Time 4 15 26 44 58 Based on these data, you could conclude that Group of answer choices b is between g and d and is 4 minutes from g e is between f and g and is 25 minutes from g g is between e and b and is 24 minutes from e none of these is correct d is between b and c and is 10 minutes from b

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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Similar questions

When the interrupted mating technique was used with five different strains of Hfr bacteria, the following orders of gene entry and recombination were observed. On the basis of these data, draw a map of the bacterial chromosome. Do the data support the concept of circularity?Hfr Strain Order1 T C H R O2 H R O M B3 M O R H C4 M B A K T5 C T K A B
Three different Hfr donor strains are mixed with separate samples of an F− strain, and the following mapping data are provided from studies of interrupted conjugation: Appearance of genes in F − cells Hfr1: Genes b+ d+ c+ f+ g+ Time* 3 5 16 27 59 Hfr2: Genes e+ f+ c+ d+ b+ Time 6 24 35 46 48 Hfr3: Genes d+ c+ f+ e+ g+ Time 4 15 26 44 58 Construct a genetic map for these genes, indicating their order on the bacterial chromosome and the distances between them.
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What is the map distance (in minutes) between these two genes?
With the technique of interrupted mating four Hfr strains were tested for the sequence in which they transmitted a number of different genes to an F strain. Each Hfr strain was found to transmit its genes is a unique sequence, as shown in the accompanying table. What is the gene sequence in the original strain from which these Hfr strains were derived?
A donor strain of bacteria with genotype leu+ gal− pro+ is infected with phages. The phage lysate from the bacterial cells is collected and used to infect a second strain of bacteria that are leu− gal+ pro−. The second strain is selected for leu+, and the following cotransduction data are obtained: Donor Recipient Selected allele Cells with cotransduced allele (%) leu+ gal− pro+ leu− gal+ pro− leu+ 47 pro+ leu+ 26 gal− Which genes are closest, leu and gal or leu and pro?
Using the technique of interrupted mating, five Hfr strains were tested for the sequence in which they transmitted several different genes to an F- strain. Each Hfr strain was found to transmit its genes in a unique sequence, as follows (only the first six genes transmitted were scored). Hfr 1 F O P R S Q Hfr 2 R P O F G Y Hfr 3 Y X W Q S R Hfr 4 W Q S R P O Hfr 5 F G Y X W Q A. For each of the Hfr strains, state which donor-gene marker should be selected in the recipients after conjugation to obtain the highest proportion of recombinants that will be Hfr? Strain 1 Strain 2 Strain 3 Strain 4 Strain 5
When the interrupted mating technique was used withfive different strains of Hfr bacteria, the following orders ofgene entry and recombination were observed. On the basisof these data, draw a map of the bacterial chromosome.Do the data support the concept of circularity?HfrStrain Order1 T C H R O2 H R O M B3 M O R H C4 M B A K T5 C T K A B
In five Hfr strains, each of which was used to build a time-of-transfer map, the genes entered the recipient cells as follows: Strain 1: S L A C T F Strain 2: N P F T C A Strain 3: T F P N U Y Strain 4: S H Y U N P Strain 5: U N P F T C Which of the following represents a correct gene map of these results? N P F T S L A C H U Y S L A C T F P N H Y U C T F P N U Y H S L A T C A L S P N U Y H F U N P C A L S F T H Y
DNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain with genotype a– b– c– d– e–. Pairs of genes are checked for co-transformation and results shown below are obtained. Based on these results, what is the order of the genes on the bacterial chromosome? please show how you worked out the order by diagraming, thank you! Pair of genes Co-transformation a+ and b+ no a+ and c+ no a+ and d+ yes a+ and e+ yes b+ and c+ yes b+ and d+ no b+ and e+ no c+ and d+ no c+ and e+ yes d+ and e+ no
Four Hfr strains are derived from an F+ strain of E. Coli to serve as donors for an interrupted-mating experiment. Use the time-table and partial map of the F+ strain (shown below) to determine the genes’ respective positions. Keep in mind that the map distances are NOT proportional, only the FIRST 5 markers are indicated per strain, and the entry times, recorded in minutes, are in parentheses. Transferred genes represent wild-type alleles. Based on the data, which gene can be located at position 5 on the map?
A donor strain of bacteria with alleles a+ b+ c+ is infected with phages to map the donor chromosome using generalized transduction. The phage lysate from the bacterial cells is collected and used to infect a second strain of bacteria that are a− b− c−. Bacteria with the a+ allele are selected, and the percentage of cells with cotransduced b+ and c+ alleles are recorded. Donor Recipient Selected allele Cells with cotransduced allele (%) a+ b+ c+ a− b− c− a+ 25 b+ a+ 3 c+ Is gene b or gene c closer to gene a? Explain your reasoning.
DNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain with genotype a− b− c− d− e−. Pairs of genes are checked for cotransformation, and the following results are obtained: Pair of genes Cotransformation Pair of genes Cotransformation a+ and b+ No b+ and d+ No a+ and c+ No b+ and e+ Yes a+ and d+ Yes c+ and d+ No a+ and e+ Yes c+ and e+ Yes b+ and c+ Yes d+ and e+ No On the basis of these results, what is the order of the genes on the bacterial chromosome?