You transform competent cells (70 000 cells/ml) with a plasmid containing an antibiotic resistance gene and spread 1 ml of the transformed cells onto plates that select for transformants. If 35 colonies grow, what is your transformation rate? cross out а. 0.0005% cross out b. 0.05% cross out С. 20% d. 2000 cells/ml cross out e. You cannot calculate cross out transformation rate with these numbers.

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications
Section: Chapter Questions
Problem 3TYK: Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning...
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You transform competent cells (70 000 cells/ml) with a plasmid containing an
antibiotic resistance gene and spread 1 ml of the transformed cells onto plates that
select for transformants. If 35 colonies grow, what is your transformation rate?
а.
cross out
0.0005%
O b.
cross out
0.05%
cross out
С. 20%
d. 2000 cells/ml
cross out
е.
You cannot calculate
cross out
transformation rate with these
numbers.
Transcribed Image Text:You transform competent cells (70 000 cells/ml) with a plasmid containing an antibiotic resistance gene and spread 1 ml of the transformed cells onto plates that select for transformants. If 35 colonies grow, what is your transformation rate? а. cross out 0.0005% O b. cross out 0.05% cross out С. 20% d. 2000 cells/ml cross out е. You cannot calculate cross out transformation rate with these numbers.
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