Analytical Reflective Essay

1. Two pieces of chromatography paper are trimmed so they are exactly 20.5 cm by 11 cm. The paper is handled by its edges only to avoid contamination from finger oils. 2. The mobile phase is prepared by dissolving a pinch of salt in 50 mL of room temperature distilled water. The salt is stirred until completely dissolved and set aside to use in step 12. 3. Enough mobile phase is transferred to the 400-600 mL beaker so that the liquid level is no more than 5 mm above the bottom of the beaker. The

3.6 Interferences study The influence of various substances as compounds potentially interfering with the determination of risperidone was studied under optimum conditions with 300.0 μM risperidone at pH 7.0. The potentially interfering substances were determined from the group of substances commonly detected with risperidone in pharmaceuticals. The tolerance limit was specified as the maximum concentration of the interfering substance that caused an error of less than ±5% in the measurement of

When light is seen through the human eye, all the colors that are seen are those that are not absorbed by the substance. Spectrophotometry is the method used to measure how much a substance absorbs light by determining the intensity of the light. In 1852, The Beer-Lambert law of A= εlc (where A is the absorptivity, ε is the molar absorptivity, l is the path length of the cell, and c is the concentration) was created. The law shows that the absorptivity of a chemical substance is directly proportional

Thin-Layer Chromotography Discussion and Conclusion Easton Montgomery Discussion: In thin-layer chromatography a liquid is pumped across a bed of particles. The liquid that is pumped across is called the mobile phase and the particles are the stationary phase. A mixture of the molecules that will be separated is put into the mobile phase. Thin-layer chromatography tells you/helps you determine the number of compounds in a mixture, the purity of a compound, and the identity of compounds if you have

Instrument Operating Instruction 1. Ensure that the instrument Mate 100 is turned off. 2. Connect the power cord and then turn on the Mate 100. 3. Once the Mate 100 is turned on, the Main Screen will display. 4. On the Main Screen, select the Setting Screen. 5. On the Setting Screen, you can make the wanted concentration by increasing or decreasing of ▲▼ button. 6. Verify the density and viscosity of the original pure oil, and then make a set of supplying time of the pure oil. 7. At this time, you

Abstract: Ion exchange chromatography is used to separate molecules base on their electrical charge and interpret the behavior of charged molecules in different samples. This is done by using a 2-step salt gradient, during the separation, molecules were released from the column base on how strong they are, the weaker substance was released first. While, in the gel filtration molecules are separated base on their size and shape using a matrix porous which allows larger molecules to be release in the

The objective of this “Chromatography Lab” is to determine the number of substances and their Rf values present as dyes in different markers/inks. Chromatography deals with physically separating a mixture into it’s very own components. Chromatography is used for keeping compounds from mixtures. The process that is involved in it, is separating out a mixture of chemicals, that are most likely either in a liquid or gas form. This process allows the chemicals/substances, to past each other, which is

Thin Layer Chromatography (TLC) is an extremely useful technique for monitoring reactions. It is also used to determine the proper solvent system for performing separations using column chromatography. TLC uses a stationary phase, usually alumina or silica, that is highly polar (standard) or non-polar (reverse phase), and a mobile phase, some solvent whose polarity you will choose. In 5.301, and in most lab applications, you will use standard phase silica plates. You will apply your reaction mixture

Aim The objective of this experiment was to separate a mixture of two compounds (ferrocene and acetylferrocene) using adsorption column chromatography. Introduction Adsorption column chromatography is a technique that uses a solid stationary phase which is fixed and a liquid mobile phase that moves slowly through the packed column. This technique uses the property of polarity as the compounds have a greater affinity towards the respective phases (either stationary or mobile) which leads the compounds

What do the Rf values reveal and conclusion The Rf Values highlight that the separate of chlorophyll evidently works better on Paper chromatography due to the polar and nonpolar molecules and depends on how soluble and less soluble the compounds are as polar molecules only dissolve in polar solvents and nonpolar only dissolve in nonpolar solvents this could be why the chlorophyll didn’t travel as far on the silica plate due to the certain solvent what was used. Additionally, as the solvent reaches