E. coli

Transformation of the pAMP to the E. coli showed growth in all plates that did not contain ampicillin, which was to be expected as E. coli with or without the ampicillin resistant DNA would be expected to survive in an environment that did not contain the ampicillin. Additionally, E. coli that was not transformed did not survive in plates containing ampicillin; a fact that we had already predicted as the bacteria has no genes protecting it from the deadly antibiotic. However, we found slightly different

Erick Espino Dr. White Bio 1B section 15 29 April 2016 MIC Effectiveness of Tetracycline on E. coli Abstract Tetracycline is a common antibiotic that is used to treat many patients that have infections such as urinary and respiratory infections. The objective of this research was to analyze the effects of Tetracycline on E.coli, in order to determine if it would have a dose response to the sensitive strain DH5Alpha. For the experiment, we had to prepare a bacterial Serial Dilution based on our

The experimental part of the lab consists of setting up the materials needed. A sample of E.coli and a solution of calcium chloride are first obtained and placed in different test tubes. 630µL of Calcium Chloride (CaCl2) are then removed from the test tube and inserted into the test tube containing E.coli cells (Alberte et al., 2012). The newly formed substance of Calcium Chloride and E.coli is then mixed and incubated in ice for 10 minutes, making the cells more competent. Two test tubes are obtained

order to test how the E. coli cell genetically transforms when introduced with a gene transcribed for GFP (green fluorescent protein). The green fluorescent protein can be found in jellyfish; it is what makes the jellyfish glow in the depths of the ocean. During Genetic Transformation, a plasmid is often introduced: a DNA molecule implemented as a vector in relation to the cell. The plasmid introduced in this lab is called pGLO. The pGLO will introduce the GFP gene to the E. coli cell, hopefully transforming

Every year over 96,000 people contract E.Coli, 3,200 of those people are hospitalized for E.Coli, and over 31 people die each year from E.Coli (Food News). E.Coli (otherwise known as Escherichia coli O157:H7) is a disease that is spread in many ways, the most common way being raw and uncooked food in the restaurant being served to the customer. Another way E.Coli is spread is through contact human/animal feces. E.Coli can be prevented, here are some ways to do so; Cook all meats to at least 160 degrees

Last summer 2011, Germany stumbled upon an outbreak of a rare species of E. coli O104:H4 (Pennington 2011). The causative strains appeared to have features from two different E. coli pathotypes of enteroaggregative E. coli (EAEC) and shiga-toxin-producing E. coli (STEC) called enterohaemorrhagic E. coli (EHEC) (Bloch, Felczykowska and Nejman-Faleńczyk 2012). The main effect from this strain causes major incident of gastroenteritis and unusual develop of haemolytic-uremic syndrome (HUS) among the

nothing so I had to narrow down the search and searched for “bacterial-borne food illnesses.” This led me to most bacterias that caused illnesses from either contaminated food or food that were not properly prepared. On this list I found that Escherichia coli was the suspect, except that the general E.coli bacteria is usually a sorbitol-fermenter and the only exception is one of the strains called “E.coli 0157:H7.” I confirmed this by searching up the sorbitol fermentation and found the sorbitol-MacConkey

Bacterial Transformation is the process by which a foreign plasmid is introduced into bacteria and for the bacteria to amplify the plasmid in order to make large quantities of it. This process has allowed scientists to link genes from different sources of bacterial plasmids, so that the organisms that have been transformed, their cells carry beneficial traits. In this experiment, we used harmless E.coli as the bacteria that was transformed to have the plasmid that had 2 genes: gene for resistance

Escherichia coli (or E. coli) is a Gram-negative, rod-shaped, facultative anaerobic bacterium found in the intestinal tract of most species of mammals and birds. E. coli cells contain pili and a flagellum. As a Gram-negative bacterium, they also contain lipopolysaccharides in the outer cell membrane. Refer to Appendix 1 for a labelled diagram of an E. coli cell. The taxonomy of Escherichia coli is as follows: Domain Bacteria Kingdom Eubacteria Phylum Proteobacteria Class Gammaproteobacteria Order

Results: From the results table we notice that on an average Amoxicillin is the most effective in inhibiting the growth of the strains of E.Coli bacteria with bleach coming in as second effective. In some cases Isocol had little or no effect to the bacteria culture growth, showing some results similar to the controlled variable. However on average it was still the least effective, even though like Dettol, Isocol is classified as an antiseptic chemical. In saying this however when looking at the