High performance team

readily dissolve in the mobile phase and will feel no attraction to the polar stationary phase, so the mixture will travel to the top of the stationary phase. In another scenario of a non-polar mobile phase and a polar mixture, the mixture will have a high attraction for the polar water molecules, so it will dissolve in the stationary phase rather then travel up the stationary phase with the mobile

Interestingly, studies from our laboratory and others have demonstrated a stereochemical dependence for dSp processing by enzymes in ODNs. The first instance of a dSp diastereomer difference was reported from our laboratory when Klenow Fragment (exo-) was allowed to insert dATP opposite the isomers for which dSp1-containing ODNs were found to be the more favorable substrate for insertion and bypass.32 This study was extended to in vivo experiments by Neeley, et al. to include pol II and pol IV

PART A - HPLC METHOD DEVELOPMENT AND QUANTITATIVE ANALYSIS Aim-To investigates the effect of changing the polarity of mobile phase on Retention time (RT) and Column capacity (k’). Results- Chromatogram for 60% methanol Tm- 1.597 Tables- For Retention time of compounds for 60% and 30% methanol. Table 1- Compound tR Xanthine 1.777 3-methylxanthine 2.500 Theobromine 2.612 Theophyline 2.798 Caffeine 3.865 Table1. shows retention time of five different compounds for 60% methanol. Table 2- Compound

N-(phosphonomethyl) glycine known as glyphosate is the world’s best-selling chemical herbicide.  Usually, it is combined with surfactants called POEA in order to improve the efficiency. ("Glyphosate | Surfactants: A Threat To Fish And Frogs?") The use as herbicide of glyphosate was discovered by Monsanto chemist John E. Franz in 1970(PENN & LYNCH, 1982). He found out that glyphosate can block the enzyme that plants need to create amino acids and other important plant metabolite. Once the glyphosate

RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Pioglitazone and Glimepiride bulk drug and in its pharmaceutical dosage form. Sujatha Samala,et.al., A simple and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of glimepiride in rat serum. The assay involves one step liquid-liquid extraction with methanol. Gliclazide was used as an internal standard. Chromatographic separation

Affinity Chromatography Lab report By- Pratibha Chaudhari 9/28/2017   Introduction- Affinity chromatography involves the property of bio-recognition for the separation of proteins based on the reversible interaction between the protein and the specific ligand bound to a chromatography matrix. It enables us to purify bio-molecule on the basis of its biological function and its chemical structure. The goal of the experiment was to gain hands-on experience in protein purification by

Advantages of UPLC: UPLC is more sensitive and selective with rapid resolving power and high-resolution performance. It decreases process cycle time and persuade quality of end-product with reduced run time and decreased cost of operation. Through the use of a novel column material of very small particle size, it provides quick analysis and increases sensitivity. It reduces the solvent consumption and raises sample throughput and also supports real-time analysis in step with manufacturing processes

Chromatography is a separation technique that is used to separate the components of a mixture (BBC, 2014). Chromatography is used to identify chemicals or colours in food, ink, and dyes to name a few (BBC, 2014). Chromatography has a stationary phase and a mobile phase. The stationary phase doesn’t move and consists of a solid, or a liquid on a solid (University of California-Davis, 2013). The mobile phase moves up through the stationary phase and consists of a liquid or a gas (University of California-Davis

Crude Extract In order to produce crude extract, bovine tissue was obtained, precisely minced to exclude extra fat, and then blended with pH 7.2 phosphate buffer. The purpose of blending the tissue with the buffer was to pulverize the cells, causing them to release their contents evenly—most importantly lactate dehydrogenase (LDH)—into the solution. Since other cell components such as proteases which reduce LDH were also released from the lysed cells, the slurry was kept on ice to minimize their

The objective of the current study was to develop and validate a simple, accurate, precise and selective stability-indicating gradient reverse phase high performance liquid chromatographic method for simultaneous estimation of Ofloxacin (OFL) and Cefixime (CEF) in pharmaceutical formulation in presence of degradation products. The chromatographic separation of Ofloxacin and Cefixime was achieved on Shimadzu LC-20AT series HPLC having C18-ODS bonded column (250 ×4.6 mm, 40 °C, 10 μL) using UV/Visible